Anti-Allergic Effect of Oroxylin A from Oroxylum indicum Using in vivo and in vitro Experiments

Islam

Evidence-Based Complementary and Alternative Medicine

et al. 2019

The stem bark of Oroxylum indicum (O. indicum) was aimed at testing for anti-inflammatory, antiulcerative, antihyperglycemic, and antidyslipidemic activities. Liver enzyme concentration (SGPT, SGOT) had also been assessed. After being extracted in organic solvent, 3 distinct doses, 100, 200, and 400 mg/kg b.w. (p.o.), were used. For edema formation 0.1 ml carrageenan at a dose of 1% w/v was injected into paw of left hind. It showed a fall of edemas 37.50%, 48.34%, and 55.83% while used doses were 100, 200, and 400 mg/kg b.w. (p.o.) individually. The EtOH extract of O. indicum (50%) and its fractions PET, CLF, EtOAc, and nBUT were studied against ethanol-induced gastric mucosal damage. Only PET and n-BuOH exhibited the highest percentage of protection and were 96% and 99%, respectively, persuaded by ethanol. In OGTT glibenclamide revealed reduction of glucose level to 7.55 ± 0.22 mmol/L from 10.57 ± 0.32 mmol/L after 30 minutes. Antihyperglycemic activities were assessed for 8- and 12-week duration in diabetic rats. Glibenclamide reduced glucose level from 33.50±0.31 to 7.90±0.19 mmol/L in 12 weeks. In 12 and 8 weeks, combination therapy lowered blood glucose level to a normal extent by 79% and 61% individually. In antidyslipidemic activities after 12-week treatment, it revealed simvastatin; MEOI (400 mg/kg b.w.) and combination of both reduced TC level by 44%, 28%, and 48% consequently followed by TG and LDL. In 8-week treatment, HDL levels were increased by 34%, 13%, and 36%, and in 12 weeks increased by 36%, 8%, and 38% consequently. Liver enzyme concentration after 12 weeks of treatment with glibenclamide, 400 mg/kg b.w. (p.o.) of MEOI and combination of both, exhibited the fact that concentration of SGPT showed downturn by 43.23%, 8.01%, and 54.86% and SGOT by 42.40%, 5.31%, and 44.85%. This study remarked that O. indicum has anti-inflammatory, antiulcer, antidiabetic, and antidyslipidemic potentials but has no ameliorative effect on liver enzyme.

Section: Discussionsupporting

confidence: 89%

Ethnopharmacological Inspections of Organic Extract ofOroxylum indicumin Rat Models: A Promising Natural Gift

Islam

Evidence-Based Complementary and Alternative Medicine

et al. 2019

“…Synthesized cDNA products, primers for each gene, and Promega Go‐Taq DNA polymerase (Madison, WI) were used for PCR. Specific primers and PCR conditions were as previously described (Lee, Kang, Park, Huang, & Im, ; Park et al, ). Aliquots (7 μl) were electrophoresed in 1.2% agarose gels and stained with StaySafe ™ Nucleic Acid Gel Stain (Real Biotech Corporation, Taipei, Taiwan; Kang et al, ).…”

Section: Methodsmentioning

confidence: 99%

Blockage of sphingosine‐1‐phosphate receptor 2 attenuates allergic asthma in mice

Park

2019

British J Pharmacology

Background and Purpose: Sphingosine-1-phosphate 2 (S1P 2 ) receptors have been implicated in degranulation of mast cells. However, functions of S1P 2 receptors have not been investigated in an in vivo model of allergic asthma.Experimental Approach: Using an ovalbumin (OVA)-induced asthma model, the function of S1P 2 receptors was evaluated in S1P 2 -deficient mice or in mice treated with JTE-013, a selective S1P 2 antagonist. Bone marrow-derived dendritic cells (BMDCs) were used to investigate the roles of S1P 2 receptors in dendritic cell maturation and migration.Key Results: Eosinophil accumulation and elevated Th2 cytokine levels in bronchoalveolar lavage fluid and inflamed lung tissues were strongly inhibited by administration of JTE-013 before OVA sensitization, before OVA challenge, and before both events. In S1P 2 -deficient mice, allergic responses were significantly lower than in wild-type mice. LPS-and OVA-induced maturation of BMDCs was significantly blunted in dendritic cells from S1P 2 -deficient mice and by treatment with JTE-013.Migrations of immature and mature BMDCs were also dependent on S1P 2 receptors.It was found that OVA-challenged mice into which in vitro OVA primed BMDCs from S1P 2 -deficient mice were adoptively transferred, had less severe asthma responses than OVA-challenged mice into which OVA-primed BMDCs from wild-type mice were adoptively transferred.Conclusions and Implications: Pro-allergic functions of S1P 2 receptors were elucidated in a murine asthma model. S1P 2 receptors were involved not only in maturation and migration of dendritic cells in the sensitization phase but also in mast cell degranulation in the challenge phase. These results suggest S1P 2 receptor as a therapeutic target for allergic asthma.

Mediators of Inflammation

“…We gathered three extracts that associated with this process: Oroxylin A [ 43 ], Bakkenolide B [ 66 ], and Petatewalide B [ 67 ]. The first one is isolated from Scutellaria baicalensis while the next two are from Petasites japonicus .…”

Section: Basic Researchesmentioning

confidence: 99%

Herbal Medicines for Asthmatic Inflammation: From Basic Researches to Clinical Applications

Liu

Xuan

Ying

et al. 2016

Asthma is one of the most common chronic inflammatory disorders, associated with reversible airflow obstruction, airway hyperresponsiveness, and airway remodeling. This disease has a significant impact on individuals, their families, and society. Standardized therapeutics such as inhaled corticosteroid in combination with long acting β2 agonist have been applied for asthma control; however, complementary and alternative medicines, especially herbal medicines, are still widely used all over the world. A growing body of literature suggests that various herbals or related products might be effective in inhibiting asthmatic inflammation. In this review, we summarize recent advances about the mechanistic studies of herbal medicines on allergic airway inflammation in animal models and their potential application into clinic for asthma control.