Anti-Amnesic Effect of Methanolic Leaf Extract of Tecoma stans: An Experimental Study in Rodents

M, Ganga Raju; Gouthami, K; Reddy, N V L Suvarchala; Nicholas, Michaela

doi:10.5530/jyp.2020.12s.54

Cited by 6 publications

(2 citation statements)

References 14 publications

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“…All the constituents were found to have interventions as direct targets with TNF-alpha, Cyclooxygenase1 and 2, Acetylcholinesterase, Muscarinic acetylcholine receptor M5, and Neuronal acetylcholine receptor protein alpha-7 subunit. And interventions as possible indirect targets with nuclear factor NF-kappa-B complex, Cyclooxygenase 1 and 2, Nuclear factor NFkappa-B p65 subunit, GABA-A receptor -alpha-1/beta-2/gamma-2 Acetylcholinesterase, and Acetylcholine receptor-alpha1/beta1/delta /gamma [25].…”

Section: Discussionmentioning

confidence: 99%

In silico and in vivo Biological Evaluation of Chenopodium quinoa on Scopolamine Induced Memory Impairment in Mice

Raju¹,

Mondal²,

Reddy³

et al. 2022

JALSI

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Aims: The present research is focused on screening in vivo and insilico anti- amnesic activity of the ethanolic extract of seeds of Chenopodium quinoa. To establish the exact the mechanism of action, the phytochemical constituents of the extract were subjected to in silico studies using schrodinger and pass software. Methodology: The powdered material of seeds of Chenopodium quinoa were dried and extracted with ethanol by soxhlation technique. In vivo evaluation of anti- amnesic activity of the ethanolic extract of seeds of Chenopodium quinoa was carried using Transfer latency by Elevated plus maze, Novel object recognition test and Passive avoidance time by cook’s pole climbing apparatus. The major phytoconstituents were subjected to molecular docking using PDB ID: 1EVE, 6N33, 2FV5 and 6W2X. Prediction studies for biological activities, adverse effects, direct and possible targets and using PASS (Predictions of activity spectra for substances) online software. Results: The extract was administered at doses (200 mg/kg and 400 mg/kg) showed significant anti- amnesic activity. The prolongation of the transfer latency time (secs) in the elevated plus-maze test is as an indicator for impairment of learning and memory. In Novel objective recognition test the time of memorizing the familiar object with that of the novel object and time taken for each animal to recognize the novel object compare to familiar object is noted. The results revealed that Quercetin, Beta sitosterol, Kaempferol, Dimethyl sulphide, Myristic acid, Palmitic acid, Stigmasterol, Lenolenic acid, Pentadecanoic acid, Tocopherols, Arachidonic acid and standard Donepezil have got highest glide scores against PDB ID: 1EVE, 6N33, 2FV5 and 6W2X. The ADME results revealed the higher oral bioavailability. The major phytoconstituents were subjected to prediction studies for biological activities, adverse effects, direct and possible targets and using PASS (Predictions of activity spectra for substances) online software. Conclusion: From in vivo and in silico results it is evident that ethanolic extract seeds of Chenopodium quinoa possessed significant anti- amnesic activity.

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Section: Discussionmentioning

confidence: 99%

In silico and in vivo Biological Evaluation of Chenopodium quinoa on Scopolamine Induced Memory Impairment in Mice

Raju¹,

Mondal²,

Reddy³

et al. 2022

JALSI

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“…The phytochemical constituent identified in the ethanolic extract of Chenopodium quinoa seeds are saturated fatty acid, phenols, sterols, flavonoids, terpenoids, tannins, proteins and anti-oxidant [12]. α-linolenic acid increases hippocampal m-RNA levels and specific phospholipase A2 encodinggene in brain and Aβ 1-42 Inhibited Aβ oligomerization decrease tau phosphorylation and pro-apoptotic proteins that leads in improvement in the cognitive function through the activation of extracellular signal-regulated kinases (ERK) and Akt signaling in the rat model increase the basal activity score by interrupting the beam of light in the arena [13]. P-Coumarin activates the GABA-A receptor as it is a phenolic compound and it shows high anti-oxidant property which help in decreasing the oxidative stress and holding the rod for longer time reducing the fall of time [14].…”

Section: Fall Off Time By Rotarod Apparatusmentioning

confidence: 99%

Evaluation of Chenopodium Quinoa Extract on Diazepam Induced Memory Impairment in Animal Models

Raju¹,

Mondal²,

V.³

et al. 2021

JAMPS

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Aim: The present study explored the pharmacological and insilico studies of ethanolic seed extract of Chenopodium quinoa. Materials and Methods: The screening of in vivo anti- amnesic activity of the ethanolic seed extract of Chenopodium quinoa was carried out using Actophotometer and Rotarod apparatus. The extract exhibited active constituents like myristic acid, palmitic acid, eicosadienoic acid, pentadecanoic acid, tocopherols, stigmasterol, β-sitosterol, quercetin, benzoic acid, kaempferol, arachidonic acid, benzofuran and 2-bromodecanoic acid. In silico approaches like docking studies (Mcule software) and Ramchandran plot (procheck), online softwares were used in the study to establish mechanism of action of active constituents present in the extract. Results: The extract treated groups at doses (200 mg/kg and 400 mg/kg, bd.wt) showed significant anti-amnesic activity. The basal activity score in actophotometer is as an indicator for impairment of learning and memory. Fall of time by rotarod is used to evaluate learning and memory in rodent models of CNS disorders as in case of amnesia. The results revealed that quercetin, kaempferol, myristic acid, palmitic acid, stigmasterol, lenolenic acid, pentadecanoic acid, tocopherols, arachidonic acid and standard donepezil have got highest glide scores against selected Protein Data Bank (PDB ID): 1EVE, 2FY4, 7CUM and 3EJ8. According to Ramachandran plot a good quality model would be expected to have over 90% in the most favoured region. Proteins like PDB ID: 1EVE, 2FY4, 7CUM and 3EJ8 showed almost 80-90% favoured region which clearly indicates that the selected proteins in the present study are of good quality. Conclusion: Our research unveiled that ethanolic seed extract of Chenopodium quinoa possessed significant anti- amnesic activity.

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Tecoma stans (L.) Juss. ex Kunth

Das,

Sahoo,

Dash

et al. 2024

Medicinal and Aromatic Plants of the World

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Anti-Amnesic Effect of Methanolic Leaf Extract of Tecoma stans: An Experimental Study in Rodents

Cited by 6 publications

References 14 publications

In silico and in vivo Biological Evaluation of Chenopodium quinoa on Scopolamine Induced Memory Impairment in Mice

In silico and in vivo Biological Evaluation of Chenopodium quinoa on Scopolamine Induced Memory Impairment in Mice

Evaluation of Chenopodium Quinoa Extract on Diazepam Induced Memory Impairment in Animal Models

Tecoma stans (L.) Juss. ex Kunth

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