Anti- and pro-oxidant effects of quercetin stabilized by microencapsulation on interstitial cells of Cajal, nitrergic neurons and M2-like macrophages in the jejunum of diabetic rats

Vieira-Frez, Flávia Cristina; Sehaber-Sierakowski, Camila C.; Perles, Juliana Vanessa Colombo Martins; Bossolani, Gleison Daion Piovezana; Verri, Waldiceu A.; Nascimento, Rafael; Guarnier, F.A.; Bordini, Heloíza Paranzini; Blegniski, F.P.; Martins, Heber Amilcar; Souza, Sara Raquel Garcia de; Lima, Fabiana Galvão da Motta; Lima, Mariana Machado; Silva, Bruna Thais; Iwanaga, Camila Cristina; Zanoni, Jacqueline Nelisis

doi:10.1016/j.neuro.2020.01.011

Cited by 18 publications

(8 citation statements)

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“…In view of the important biological role that antioxidant compounds play in improving human health [ 51 , 82 ], the discovery of alternative food sources is of great interest. The bioactivity of phenolic compounds [ 83 ], whether anti- or pro-oxidant [ 84 ], reveals the need for profiling these compounds in new food matrices, verifying their applicability in biotechnological and functional terms. Table 4 shows the MF phenolic profile, ascorbic acid content, total phenolic and flavonoid content, and antioxidant activity.…”

Section: Resultsmentioning

confidence: 99%

Physical, Nutritional, and Bioactive Properties of Mandacaru Cladode Flour (Cereus jamacaru DC.): An Unconventional Food Plant from the Semi-Arid Brazilian Northeast

Martins

Medeiros

Silva

et al. 2022

Foods

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In this study, we evaluated the physical, nutritional, and bioactive properties of mandacaru cladode flour (Cereus jamacaru DC.). The granulometric profile revealed particles with non-uniform geometry, flakiness, a rectangular tendency, and a non-homogeneous surface, with particle sizes ranging from 20 to 60 µm. The flour presented low water activity (0.423), a moisture content of 8.24 g/100 g, high ash (2.82 g/100 g), protein (5.18 g/100 g), and total carbohydrate contents (74.48 g/100 g), and low lipid contents (1.88 g/100 g). Mandacaru flour is an excellent source of insoluble dietary fiber (48.08 g/100 g), calcium (76.33%), magnesium (15.21%), and potassium (5.94%). Notably, 1H NMR analysis revealed the presence of N-methyltyramine. Using HPLC chromatography, glucose was identified as the predominant sugar (1.33 g/100 g), followed by four organic acids, especially malic acid (9.41 g/100 g) and citric acid (3.96 g/100 g). Eighteen phenolic compounds were detected, with relevant amounts of kaempferol (99.40 mg/100 g), myricetin (72.30 mg/100 g), and resveratrol (17.84 mg/100 g). The total phenolic compounds and flavonoids were 1285.47 mg GAE/100 g and 15.19 mg CE/100 g, respectively. The mean in vitro antioxidant activity values were higher using the FRAP method (249.45 µmol Trolox TEAC/100 g) compared to the ABTS•+ method (0.39 µmol Trolox TEAC/g). Finally, the ascorbic acid had a content of 35.22 mg/100 g. The results demonstrate the value of mandacaru as a little-explored species and an excellent matrix for the development of flours presenting good nutritional value and bioactive constituents with excellent antioxidant potential.

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Section: Resultsmentioning

confidence: 99%

Physical, Nutritional, and Bioactive Properties of Mandacaru Cladode Flour (Cereus jamacaru DC.): An Unconventional Food Plant from the Semi-Arid Brazilian Northeast

Martins

Medeiros

Silva

et al. 2022

Foods

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“…The changes in the intestinal wall may be related to factors such as GIT dysmotility (Bytzer et al, 2002). Due to the significant differences presented in the small intestine length in the study groups (NQ10, NQ100, D, DQ10 AND DQ100), the use of a corrector factor avoids distortions in the neuronal density results (i. e. neuronal dispersion or concentration), that are very common for many physiopathological conditions such as aging, cancer and diabetes (Gamage et al, 2013;Phillips and Powley, 2007;Vicentini et al, 2017;Vieira-Frez et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…After the euthanasia, the width (ileum) and length of the small intestine were measured and then the average area in square centimeters (cm 2 ) was calculated. A correction factor was stipulated by comparing average areas with the control group, being this factor represented by the ratio between the intestine areas of the experimental groups (NQ10, NQ100, D, DQ10 and DQ100) and the intestine area of the control group (N) (Table 2) (Gamage et al, 2013;Phillips and Powley, 2007;Vicentini et al, 2017;Vieira-Frez et al, 2020). For oxidative stress techniques, the ileal segments were collected and immediately rinsed in 0.1 M PBS solution (pH 7.4).…”

Section: Neuron Density Correctionmentioning

confidence: 99%

Protective effects of quercetin-loaded microcapsules on the enteric nervous system of diabetic rats

Sehaber-Sierakowski

Vieira-Frez

Hermes-Uliana

et al. 2021

Autonomic Neuroscience

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“…A study by Spencer et al, (2003) demonstrated that the protective role of quercetin at 10μM of concentration against dermal fibroblast cells' damage caused by oxidative stress, whereas cytotoxicity was encountered in these cells, when quercetin dose was increased up to 30μM indicating that, Quercetin shows dual the effect as both pro and anti-oxidant properties depending upon the dose used [43]. In relation to these findings, Vieira-Frez et al (2020) have reported that quercetin at higher doses (100mg-1) exerted pro-oxidant property by aggravating the ROS production and further showed harmful effects in diabetic rats and at the same time, low dose administration of quercetin (10 mg/kg-1) gave rise to antioxidant property consequently protected the rats from oxidative stress [44]. It is therefore very much important to standardize the dose escalations before using quercetin in preclinical and clinical studies.…”

Section: Plos Onementioning

confidence: 96%

Hypobaric hypoxia induced renal injury in rats: Prophylactic amelioration by quercetin supplementation

et al. 2023

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The present study aims at assessing the effect of hypobaric hypoxia induced renal damage and associated renal functions in male SD rats. Further, this study was extended to explore the protective efficacy of quercetin in ameliorating the functional impairment in kidneys of rats under hypobaric hypoxia. Rats were exposed to 7620m (25000 ft.) at 25°C ±2 in a simulated hypobaric hypoxia chamber for different time durations (0h,1h, 3h, 6h, 12h, 24h and 48h) in order to optimize the time at which maximum renal damage would occur. The rats were exposed to hypoxia for 12h duration was considered as the optimum time, due to significant increase in oxidative stress (ROS, MDA) and renal metabolites (creatinine, BUN and uric acid) with remarkable reduction (p<0.001) in antioxidants (GSH) in plasma, as compared to other tested durations. Moreover, these findings were in support with the histopathology analysis of renal tissues. For optimum quercetin dose selection, the rats were administered with different doses of quercetin (25mg, 50mg, 100mg and 200mg/Kg BW) for 12h at 7620 m, 25°C ±2, 1h prior to hypoxia exposure. Quercetin 50mg/kg BW was considered as the optimum dose at which significant (p<0.001) reduction in oxidative stress levels followed by reduction in creatinine and BUN levels were obtained in plasma of the rats compared to hypoxia control rats. Quercetin prophylaxis (50mg/kg BW) stabilized the HIF-1α protein expression followed by reduced VEGF protein expression along with reduced levels of LDH (p<0.001) in the kidneys of rats compared to hypoxia control. Histopathological observations further substantiated these findings in reducing the renal tissue injury. The study findings revealed that, quercetin prophylaxis abrogates the possibility of hypobaric hypoxia induced renal injury by reducing the oxidative stress in rats.

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Anti- and pro-oxidant effects of quercetin stabilized by microencapsulation on interstitial cells of Cajal, nitrergic neurons and M2-like macrophages in the jejunum of diabetic rats

Cited by 18 publications

References 50 publications

Physical, Nutritional, and Bioactive Properties of Mandacaru Cladode Flour (Cereus jamacaru DC.): An Unconventional Food Plant from the Semi-Arid Brazilian Northeast

Physical, Nutritional, and Bioactive Properties of Mandacaru Cladode Flour (Cereus jamacaru DC.): An Unconventional Food Plant from the Semi-Arid Brazilian Northeast

Protective effects of quercetin-loaded microcapsules on the enteric nervous system of diabetic rats

Hypobaric hypoxia induced renal injury in rats: Prophylactic amelioration by quercetin supplementation

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