Anti-asthmatic Activity of an Ethanol Extract from Saururus chinensis

Abstract

As an attempt to find bioactive medicinal herbs exerting anti-asthmatic activity, the effects of an ethanol extract from the parts of Saururus chinensis were evaluated in both in vitro and in vivo. The ethanol extract of S. chinensis (ESC) inhibited generation of the cyclooxygenase-2 (COX-2) dependent phases of prostaglandin D 2 in bone marrow-derived mast cells in a concentration-dependent manner with an IC 50 value of 14.3 m mg/ml. ESC also inhibited leukotriene C 4 production with an IC 50 value of 0.3 m mg/ml. This demonstrates that ESC has COX-2/5-lipoxygenase dual inhibitory activity. In addition, this compound inhibited degranulation reaction in a dose dependent manner, with an IC 50 value of 1.3 m mg/ml. An ovalbumin induced mouse asthmatic animal model was used to determine its in vivo anti-asthmatic activity. The oral administration (50-200 mg/kg) of ESC reduced the number of infiltrated eosinophil in a bronchoalveolar lavage fluid. Furthermore, ESC (100 mg/kg) inhibited the eotaxin and IL-4 mRNA expression levels. These results suggest that the anti-asthmatic activity of S. chinensis might in part occur via the inhibition of eicosanoid generation, degranulation as well as the down regulation of IL-4 and eotaxin mRNA expression.

Protective Effects of Manassantin A against Ethanol-Induced Gastric Injury in Rats

“…Its aerial portion has been used in Korean folk medicine for the treatment of edema, gonorrhea, jaundice, and several inflammatory diseases. 10) Studies have shown that S. chinensis has various activities, including anti-inflammatory, anti-oxidant, 11,12) antiangiogenic, 13) anti-asthmatic, 14) and anti-hypertensive 15) activities, as well as therapeutic activity against atopic dermatitis. 16) Among the bioactive compounds isolated from S. chinensis, manassantin A is a major phytochemical composition that has various biological activities, including anti-inflammatory, neuroleptic, and human acyl-CoA : cholesterol acyltransferase (ACA T) inhibitory activities.…”

Anti-proliferative Neolignans from <i>Saururus chinensis</i> against Human Cancer Cell Lines

“…Activity-guided chromatographic separation of 80% EtOH extract led to isolation of eleven compounds including three active neolignans (1-3), four flavonoids (4-7), and four aristolactams (8)(9)(10)(11). The structures of 1-11 were identified as manassantin A (1), 27,28) manassantin B (2), 29) (−)-saucerneol (3), 29,30) quercetin-3-O-β-Dglucuronopyranoside (4), 31) quercitrin (5), 32) 33) isoquercitrin (7), 34) saurolactam (8), 35) aristolactam AII (9), 36) piperumbellactam B (10), 37) and aristolactam BII (11) 36) by analysis of spectroscopic data including 1 H-and 13 C-NMR data as well as by comparison of their spectroscopic data with the values reported in the literature (Fig.…”

“…1) Previous pharmacological studies on S. chinensis have reported that its extracts have anti-inflammatory, anti-oxidant, anti-asthmatic, and cytotoxic activities. [2][3][4][5][6][7][8][9][10] Phytochemical investigations on this plant have reported that it contains several types of secondary metabolites, such as lignans, neolignans, aristolactams, and flavonoids [11][12][13] that are associated with anti-inflammatory, antioxidant, cytotoxic, anti-cancer, anti-human immunodeficiency virus (HIV), and anti-Acyl-CoA : cholesterol acyltransferase (ACAT)-1, 2 activities. [14][15][16][17][18][19][20][21][22][23][24][25] A number of studies demonstrated that the compounds derived from medicinal herbs play an important role in the anti-cancer activity and have been developed as new anti-cancer drugs.…”

Amelioration of Asthmatic-Related Symptoms by an Aqueous Extract of Angelica archangelica L.

“…Mice underwent ovalbumin (OVA) sensitization and challenge using an earlier protocol with slight modifications [3,10,22]. OVA (100 μg/ml in saline, sterile filtered) was mixed with an equal volume of 0.1% (w/v) aluminum potassium phosphate (alum; Sigma), and the pH adjusted to 6.5 with 10 N NaOH.…”