Protective Effects of Manassantin A against Ethanol-Induced Gastric Injury in Rats

Abstract

Manassantin A, a neolignan isolated from Saururus chinensis, is a major phytochemical compound that has various biological activities, including anti-inflammatory, neuroleptic, and human acyl-CoA : cholesterol acyltransferase (ACAT) inhibitory activities. In this study, we investigated the protective effects of manassantin A against ethanol-induced acute gastric injury in rats. Gastric injury was induced by intragastric administration of 5 mL/kg body weight of absolute ethanol to each rat. The positive control group and the manassantin A group were given oral doses of omeprazole (20 mg/kg) or manassantin A (15 mg/kg), respectively, 1 h prior to the administration of absolute ethanol. Our examinations revealed that manassantin A pretreatment reduced ethanol-induced hemorrhage, hyperemia, and epithelial cell loss in the gastric mucosa. Manassantin A pretreatment also attenuated the increased lipid peroxidation associated with ethanolinduced acute gastric lesions, increased the mucosal glutathione (GSH) content, and enhanced the activities of antioxidant enzymes. The levels of pro-inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β were clearly decreased in the manassantin A-pretreated group. In addition, manassantin A pretreatment enhanced the levels of cyclooxygenase (COX)-1, COX-2, and prostaglandin E 2 (PGE 2 ) and reduced the inducible nitric oxide synthase (iNOS) overproduction and nuclear factor kappa B (NF-κB) phosphorylation. Collectively, these results indicate that manassantin A protects the gastric mucosa from ethanol-induced acute gastric injury, and suggest that these protective effects might be associated with COX/ PGE 2 stimulation, inhibition of iNOS production and NF-κB activation, and improvements in the antioxidant and anti-inflammatory status.

Pharmacological Effects and Toxicogenetic Impacts of Omeprazole: Genomic Instability and Cancer

“…This OME mechanism was reported for rats at doses that varied between 10 μM and 400 mg/kg, as well as 200 μM/ml in regular epithelial cell lines (MDCK) and mouse macrophage (RAW264.7) [55,56]. Reduction of lipid peroxidation (Biswas et al, 2003) Expression of superoxide dismutase (Song et al, 2016) Proton pump inhibitor Activation of the HSP70 and TGF-…”

“…In vivo and in vitro studies report inhibition of necrosis by activation of TNF-α, interleukin B [75], and proinflammatory cytokines [76]. OME at 20 mg/kg presented antioxidant activity through mechanisms associated with increased superoxide dismutase (SOD) enzyme production [77,78], as well as glutathione peroxidase (GPx) and reduced glutathione (GSH) at 30 and 40 mg/kg [51,79]. In ethanol-induced gastritis rats, OME modulated mucosal lesions through its antioxidant and anti-inflammatory activity [80].…”

“…Other mechanisms of action of OME, observed in animals and in cell cultures, relate to the anti-inflammatory marker cyclooxygenase (COX)-2 [77] and NSAIDs, which inhibit COX-1 and COX-2 that cause gastric ulcerogenic effects [82] and to the increase in the neurotrophic tyrosine kinase receptor type 2 (Ntrk2) [83]. OME also reduced TNF-α capacity (in vivo) [84].…”

Allantoin improves histopathological evaluations in a rat model of gastritis

“…As it has been shown in many studies, our results also indicated that ethanol consumption led to PGE2 decrease, and allantoin administration in gastritis groups was observed to increase PGE2 levels in the gastric tissue. Previously, it has been documented that allantoin increased the activity of COXs production pathways (21,23). Therefore, it is probable that increased COX1 and COX2 synthesis will cause increased PGE2 secretion in the stomach tissue.…”

Gastroprotective Activity of the Total Flavones from Abelmoschus manihot (L.) Medic Flowers

“…e increase in MDA content is associated with the necrosis and apoptosis of cells [24]. SOD can exert its effects by removing oxygen free radicals from the body [25]. erefore, GSH, MDA, and SOD were measured in this study to evaluate oxidative stress levels.…”