Gastroprotective Activity of the Total Flavones from Abelmoschus manihot (L.) Medic Flowers

Abstract

Background. Abelmoschus manihot (L.) Medic flower is a medicinal plant for the treatment of diseases in China. e present study was carried out to scientifically validate the gastroprotective activity and clarify the possible mechanism of the total flavones from Abelmoschus manihot (L.) Medic flowers (TFA). Methods. Gastric ulcer was induced in mice by oral administration of ethanol. e gastroprotective activity of TFA was evaluated by the gastric ulcer index and histological examinations. e gastric tissue was collected in the form of homogenate. e level of malondialdehyde (MDA) and glutathione (GSH), the activity of superoxide dismutase (SOD), and protein content were measured. Western blotting for the expression of Bax, Bcl-2, TNF-α, and NF-κB(p65) was also carried out. e effect of TFA was compared with that of standard antiulcer drug omeprazole (100 mg/kg). Results. is gastroprotective effect of TFA could be attributed to the increase in the activity of SOD and GSH and decrease in the levels of MDA and also decrease in the levels of Bax, TNF-α, and NF-κB(p65) expressions and increase in the Bcl-2 expression level. Conclusion.e findings of this study demonstrated that TFA could significantly attenuate ethanol-induced gastric injury via antioxidative, anti-inflammatory, and antiapoptotic effects.

The flower ofAbelmoschus manihot(L.)medikexerts antioxidant effects by regulating theNrf2signalling pathway in scald injury

“…According to the Traditional Chinese Medicine Dictionary (Nanjing University of Chinese Medicine, 2015), AM , a traditional Chinese medicinal herb for treating thermal injury and other skin disorders, has analgesic, anti‐inflammatory and antioxidant effects. In recent years, researchers have found that (1) the total flavonoids from AM could protect mice against CCl 4 ‐induced liver injury through antioxidant stress and anti‐inflammatory effects 21 ; (2) AM extracts could ameliorate renal inflammation and glomerular injury in adriamycin‐induced nephropathy rats by increasing the expression of TNF‐α in the kidney by inhibiting p38/MAPK signalling pathway activity in vivo 4 ; and (3) total flavonoids from AM could shorten the healing time of infected oral mucosa ulcers of guinea pigs and gastric ulcers in our laboratory 22 . Although AM is a traditional medicine for the treatment of scalds in China, no systematic scientific research has been carried out on the underlying mechanism of its therapeutic effect.…”

“…4 ; and (3) total flavonoids from AM could shorten the healing time of infected oral mucosa ulcers of guinea pigs and gastric ulcers in our laboratory. 22 Although AM is a traditional medicine for the treatment of scalds in China, no systematic scientific research has been carried out on the underlying mechanism of its therapeutic effect. Since it is extracted from plants, its safety is higher than that of general chemical drugs.…”

Effect of a Cytoprotective Dose of Dehydroleucodine, Xanthatin, and 3-Benzyloxymethyl-5H-furan-2-one on Gastric Mucosal Lesions Induced by Mast Cell Activation

“…Compound 48/80 is known to cause degranulation of connective tissue mast cells, such as peritoneal mast cells, with the release of serotonin and histamine from the cells [ 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. Ohta et al have shown that a single compound 48/80 treatment induces the release of endogenous biogenic amines through degranulation of connective tissue mast cells and the subsequent development of gastric mucosal lesions in male Wistar rats [ 39 ].…”

Dendrobium officinale Flowers Flavonoids Enriched Extract Protects against Acute Ethanol-induced Gastric Ulcers via AMPK/PI3K Signaling Pathways

“…The chemical components of D. officinale flowers are similar to those of stems with flavonoids to amount for the main bioactive compounds in its extract [20]. Zhang et al [21] reported that flavonoids could significantly attenuate ethanol-induced gastric injury mediated via their antioxidant, anti-inflammatory, and anti-apoptotic effects. In vitro and in vivo studies have shown that D. officinale plays a gastroprotective effect by inhibiting oxidative stress-induced apoptosis [22].…”