Anti-Atopic Properties of Gracillin Isolated from Dioscorea quinqueloba on 2,4-Dinitrochlorobenzene-Induced Skin Lesions in Mice

Abstract

Naturally occurring saponins have been reported to have anti-inflammatory and immunomodulatory effects. However, the effects of gracillin, a main saponin component of Dioscorea quinqueloba (D. quinqueloba), on atopic dermatitis (AD), have not been previously studied. The aim of this study was to determine whether gracillin isolated from D. quinqueloba has an anti-AD effect on 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions in SKH-1 hairless mice. Topical co-treatment of gracillin and DNCB for two weeks markedly reduced symptoms typical of AD (redness, itching, swelling and skin lichenification), decreased transepidermal water loss (TEWL) and increased skin hydration. In addition, gracillin strongly inhibited PI-induced IL-4 expression in RBL-2H3 cells and in the skins of AD mice. Our results suggest gracillin is a potential candidate for the prevention and treatment of AD and other inflammatory skin disorders.

Anti-Atopic Effect of Acorn Shell Extract on Atopic Dermatitis-Like Lesions in Mice and Its Active Phytochemicals

“…In addition, the efficacy of dupilumab, the drug that regulates IL-4, is known to be the best in the clinical trial. Previous papers have demonstrated that the extracts of natural products inhibiting IL-4 expression and some of the compounds isolated therefrom have excellent efficacy against atopic dermatitis in vivo [50,51] Therefore, compounds that inhibit IL-4 expression can be inferred to be able to ameliorate atopic dermatitis in vivo. Gallic acid and ellagic acid have previously been reported to have extensive anti-inflammatory activities, as revealed in our experiments.…”

Topical application with conjugated linoleic acid ameliorates 2, 4‐dinitrofluorobenzene‐induced atopic dermatitis‐like lesions in BALB/c mice

“…Patients with AD are known to have elevated skin pH and loss of skin hydration 36,37 . Topical DNFB reduces skin barrier function as determined by skin hydration and PH testing.…”

Gracillin shows potent efficacy against colorectal cancer through inhibiting the STAT3 pathway

“…The recently discovered cancer-promoting functions of STAT3 further emphasize the importance of targeting STAT3 activities related to the mitochondria, epigenetic regulation, cancer stem cells, premetastatic niches and more. 22,23 The STAT3-Fam3a axis has been reported to promote the progression of the muscle stem cell myogenic lineage by enhancing the mitochondrial respiration. 24 STAT3 can mediate the epigenetic silencing of tumour suppressor genes (TSG).…”

“…Dioscorea species contains a variety of substances to promote human health, such as amylose, choline, mucin, steroidal saponins and saponins. 14 Although a great deal of research has been performed on the different species of Dioscorea genus, few have addressed the patient in D. quinqueloba. Gracillin is a natural steroidal saponin component of D. quinqueloba.…”