Anti-bacterial and cytotoxic properties of plasma sprayed silver-containing HA coatings

Abstract

Silver-containing hydroxyapatite (HA) coatings have been prepared on titanium substrate by vacuum plasma spraying (VPS) method and anti-bacterial properties of the coatings were examined. Three types of bacteria stains, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, were employed in this test. The results showed that the silver-containing HA coatings exhibited significant anti-bacterial effects against the three bacteria with anti-bacterial ratios higher than 95%. The release of silver ions in the physiological environment ensured excellent anti-bacterial properties of the silver-containing HA coatings. International standard ISO 10993-12 was adopted for cytotoxicity evaluation using fibroblast cell line L929, and it was found that the cytotoxicity for the coatings ranked 0 that showed no cytotoxicity for the coatings. Hemolysis test was processed according to ASTM F 756 standard with anti-coagulated rabbit blood, and the hemolysis ratios of the coatings were below 0.4%, indicating of non-hemolysis for the coatings.

Antimicrobial activity of hemocompatible silver doped hydroxyapatite nanoparticles synthesized by modified sol–gel technique

“…It was observed that 3 % Ag-HAp exhibited maximum activity as compared to 1 and 5 % Ag-HAp for both the test organisms. Antimicrobial activity of Ag-HAp based compounds such as plasma sprayed silver containing HAp coatings on Ti plates (Chen et al 2008), magnetron co-sputtered silver containing HAp coatings (Chen et al 2006), Ag-nHAp/TiO 2 /PA66 scaffolds (Xia et al 2010), silver-hydroxyapatite/titania film on titanium plates (Mo et al 2008) and HAp self assembled calcium phosphate glasses (Simon et al 2008) has been attributed to the Ag ions released into the medium. It is well known that silver ions and silver based compounds can destroy cell membranes and cell walls of bacteria and affect their growth (Woo et al 2008;Furr et al 1994).…”

“…(Woo et al 2008;Furr et al 1994;Schreus and Rosenberg 1982;Feng et al 2000) Thus, it has recently become one of the preferred ions to confer microbial resiliency on biomaterials and medical devices (Gristina and Costerton 1985). Several in vitro studies have reported that silver ions in HAp play an important role in preventing or minimizing initial bacterial adhesions (Mo et al 2008;Chen et al 2008).…”

Nano-Ag-loaded hydroxyapatite coatings on titanium surfaces by electrochemical deposition

“…It also exhibits high thermal stability and low toxicity towards mammalian cells [18,19]. There are several methods to introduce Ag into Ca-P coatings, such as plasma spraying [20,21], ion beam-assisted deposition [22], magnetron sputtering [23], micro-arc oxidation [24] and sol -gel technology [25,26]. The first three methods are line-ofsight technologies and cannot be applied to the medical devices with a complex shape; the last two methods have the problem of controlling the distribution of Ag in the coatings.…”

Antibacterial hemostatic dressings with nanoporous bioglass containing silver

“…Ag-TiO 2 coatings also had higher antibacterial properties, with tolerable cytocompatibility. 22 A study showed that the surface antibacterial property and biocompatibility of biopolymers can be enhanced by means of plasma immersion ion implantation (PIII). Based on cell assays, the Ag PIII polyethylene samples exhibited excellent biocompatibility for cells, demonstrating that Ag PIII not only enhances antibacterial properties but also improves the cell biocompatibility of polyethylene.…”