Anti-beta-interferon antibodies inhibit the increased expression of HLA-B7 mRNA in tumor necrosis factor-treated human fibroblasts: structural studies of the beta 2 interferon involved.

May, Lester T.; Helfgott, David C.; Sehgal, Pravinkumar B.

doi:10.1073/pnas.83.23.8957

Cited by 194 publications

(70 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The human foreskin diploid fibroblast strain FS-4 was obtained from J. Vildek (New York University School of Medicine, New York); procedures for its growth in cell culture and for the IFN-p2 mRNA induction experiments have been described (1,5,12,13). In brief, FS-4 cells were grown to confluence in T-175 Falcon flasks in Eagle's minimal essential medium supplemented with 10% (vol/vol) heat-inactivated fetal bovine serum (GIBCO).…”

Section: Methodsmentioning

confidence: 99%

Rapid enhancement of beta 2-interferon/B-cell differentiation factor BSF-2 gene expression in human fibroblasts by diacylglycerols and the calcium ionophore A23187.

Sehgal¹,

Walther²,

Tamm³

1987

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

125

View full text Add to dashboard Cite

The expression in human fibroblasts of the p2-interferon (IFN-.82) gene, which is now recognized to be identical to the gene encoding B-cell differentiation factor BSF-2, is enhanced by several cytokines that affect cell growth (tumor necrosis factor, interleukin 1, platelet-derived growth factor, and fi1-interferon). We have examined the possibility that IFN-.82 gene expression is regulated through activation, by diacylglycerol, of the protein kinase C pathway. MATERIALS AND METHODSThe human foreskin diploid fibroblast strain FS-4 was obtained from J. Vildek (New York University School of Medicine, New York); procedures for its growth in cell culture and for the IFN-p2 mRNA induction experiments have been described (1,5,12,13). In brief, FS-4 cells were grown to confluence in T-175 Falcon flasks in Eagle's minimal essential medium supplemented with 10% (vol/vol) heat-inactivated fetal bovine serum (GIBCO). The cultures were refed at weekly intervals, and confluent cultures were used for experiments 6-8 days after the last medium change. Concentrated solutions of the experimental reagents were added directly to the spent medium in the cultures. 1,2-Dioctanoylglycerol (diC8) was purchased from Avanti Polar Abbreviations: diC8, 1,2-dioctanoylglycerol; H7, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride; H8, N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride; IFN, interferon; IL-1, interleukin 1; OAG, 1-oleoyl-2-acetylglycerol; TNF, tumor necrosis factor; VSV, vesicular stomatitis virus. 3663The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

show abstract

Section: Methodsmentioning

confidence: 99%

Rapid enhancement of beta 2-interferon/B-cell differentiation factor BSF-2 gene expression in human fibroblasts by diacylglycerols and the calcium ionophore A23187.

Sehgal¹,

Walther²,

Tamm³

1987

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

125

View full text Add to dashboard Cite

show abstract

“…The previously reported 26-kilodalton (kDa) protein, beta2 interferon, hybridoma plasmacytoma growth factor, and hepatocyte-stimulating factor have turned out to be identical with BSF-2 (1, 10, 18,23,26). It has been proposed to designate this molecule interleukin 6 (IL-6) (20,25).…”

mentioning

confidence: 99%

Induction of neuronal differentiation in PC12 cells by B-cell stimulatory factor 2/interleukin 6.

Satoh¹,

Nakamura²,

Taga³

et al. 1988

Mol. Cell. Biol.

361

145

View full text Add to dashboard Cite

B-cell stimulatory factor 2 (BSF-2) is a lymphokine which induces the final maturation of B cells. BSF-2 acts on a variety of cells other than B cells, and moreover, expression of BSF-2 mRNA is detected in interleukin-1 beta-stimulated glioblastoma and astrocytoma cell lines. Here, we studied the function of BSF-2 on pheochromocytoma PC12 cells, a model system for induction of neuronal differentiation. PC12 cells possess specific receptors for BSF-2. The BSF-2-stimulated PC12 cells expressed the c-fos proto-oncogene transiently, and they began to change morphologically to neurite-extending cells after several days. The number of voltage-dependent Na+ channels was also increased.

show abstract

“…It is produced by many types of cells, including CD8+ T lymphocytes, fibroblasts, monocytes, osteoblasts, megakariocytes and adipocytes (10)(11)(12)(13). Recent studies have found increased levels during the inflammatory phase in 95% of patients with local infection.…”

mentioning

confidence: 99%

Procalcitonin, C-Reactive Protein, Interleukin-6, and Soluble Intercellular Adhesion Molecule-1 as Markers of Postoperative Orthopaedic Joint Prosthesis Infections

Drago

Vassena

Dozio

et al. 2011

Int J Immunopathol Pharmacol

View full text Add to dashboard Cite

There is a universally recognized need to identify new, reliable markers of inflammation that can aid in the rapid diagnosis of orthopaedic joint prosthesis infections (OJP-Is). Since prompt diagnosis is key to timely intervention in the course of infection, different molecules have been studied. In this study, we examined three groups of patients: those with prosthesis infection, those without infection, and a third group with previous infection in whom the infection had been cleared. Four presumed markers of infection were tested: procalcitonin (PCT); C-reactive protein (CRP); interleukin-6 (IL-6); and soluble intercellular adhesion molecule-1 (sICAM-1). The results showed that PCT cannot be considered as a good marker of periprosthetic infection as no statistically significant difference in serum PCT levels emerged between patients with infection and controls or patients without infection. In contrast, both sICAM-1 and CRP may be considered as good markers of infection, as measurement of their levels allowed us to distinguish between patients with and without infection, and between patients with infection and those with previous infection, since marker levels quickly returned to baseline values after clearance of the infection. IL-6 was found to be a good marker for inflammation, as it distinguished between patients with infection and the other groups. In the patients with previous infection, the IL-6 values remained high versus the controls but lower and with a statistically significant difference versus the patients with infection. Further studies are needed to determine the cut-off value of IL-6 between patients with infection and those with previous infection.

show abstract

Anti-beta-interferon antibodies inhibit the increased expression of HLA-B7 mRNA in tumor necrosis factor-treated human fibroblasts: structural studies of the beta 2 interferon involved.

Cited by 194 publications

References 29 publications

Rapid enhancement of beta 2-interferon/B-cell differentiation factor BSF-2 gene expression in human fibroblasts by diacylglycerols and the calcium ionophore A23187.

Rapid enhancement of beta 2-interferon/B-cell differentiation factor BSF-2 gene expression in human fibroblasts by diacylglycerols and the calcium ionophore A23187.

Induction of neuronal differentiation in PC12 cells by B-cell stimulatory factor 2/interleukin 6.

Procalcitonin, C-Reactive Protein, Interleukin-6, and Soluble Intercellular Adhesion Molecule-1 as Markers of Postoperative Orthopaedic Joint Prosthesis Infections

Contact Info

Product

Resources

About