Anti-biofilm Activity of a Lytic Phage Against Vancomycin-Resistant Enterococcus faecalis

Goodarzi, Forough; Hallajzadeh, Masoumeh; Sholeh, Mohammad; Talebi, Malihe; Mahabadi, Vahid Pirhajati; Amirmozafari, Nour

doi:10.30699/ijp.2022.541855.2760

Cited by 9 publications

(9 citation statements)

References 40 publications

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“…On the other hand, the results obtained for phages 3e confirm those found in Goodarzi et al studies (2022) describing the effectiveness of phages against mature E. faecalis biofilms [28]. The research conducted by Hanlon G.W.…”

Section: Discussionsupporting

confidence: 85%

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How Do Phages Disrupt the Structure of Enterococcus faecalis Biofilm?

Moryl,

Różalski,

de Figueiredo

et al. 2023

IJMS

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Biofilms are composed of multicellular communities of microbial cells and their self-secreted extracellular polymeric substances (EPS). The viruses named bacteriophages can infect and lyze bacterial cells, leading to efficient biofilm eradication. The aim of this study was to analyze how bacteriophages disrupt the biofilm structure by killing bacterial cells and/or by damaging extracellular polysaccharides, proteins, and DNA. The use of colorimetric and spectrofluorimetric methods and confocal laser scanning microscopy (CLSM) enabled a comprehensive assessment of phage activity against E. faecalis biofilms. The impact of the phages vB_Efa29212_2e and vB_Efa29212_3e was investigated. They were applied separately or in combination on 1-day and 7-day-old biofilms. Phages 2e effectively inhibited the growth of planktonic cells with a limited effect on the biofilm. They did not notably affect extracellular polysaccharides and proteins; however, they increased DNA levels. Phages 3e demonstrated a potent and dispersing impact on E. faecalis biofilms, despite being slightly less effective than bacteriophages 2e against planktonic cells. Phages 3e reduced the amount of extracellular polysaccharides and increased eDNA levels in both 1-day-old and 7-day-old biofilm cultures. Phage cocktails had a strong antimicrobial effect on both planktonic and biofilm-associated bacteria. A significant reduction in the levels of polysaccharides, proteins, and eDNA in 1-day-old biofilm samples was noted, which confirms that phages interfere with the structure of E. faecalis biofilm by killing bacterial cells and affecting extracellular polymer levels.

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Section: Discussionsupporting

confidence: 85%

“…et al ( 2001) also indicates that the sensitivity of Pseudomonas aeruginosa biofilm to phage F116 does not diminish with time or the age of the biofilm. This phage remained effective even against a 20-day-old biofilm [28,29].…”

Section: Discussionmentioning

confidence: 97%

How Do Phages Disrupt the Structure of Enterococcus faecalis Biofilm?

Moryl,

Różalski,

de Figueiredo

et al. 2023

IJMS

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“…The methodology for the biofilm assay was adapted from a previous study ( Goodarzi et al, 2022 ). To establish a biofilm, fill each well of a 96-well plate with 100 μL of BHI medium containing an overnight culture of E. faecalis .…”

Section: Methodsmentioning

confidence: 99%

Characterization of an Enterococcus faecalis bacteriophage SFQ1 as a potential therapeutic agent

Song,

Sheng,

Tan

et al. 2023

Front. Microbiol.

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Enterococcus faecalis is a well-established resident of the human gastrointestinal tract and is also a major cause of human infections. Unfortunately, therapeutic options for E. faecalis infections remain limited, particularly with the emergence of vancomycin-resistant strains in hospital settings. Consequently, there has been a renewed interest in phage therapy as an alternative to antibiotics. In this study, we have isolated a bacteriophage, vB_EfaS-SFQ1, from hospital sewage, which effectively infects E. faecalis strain EFS01. Phage SFQ1 is a siphovirus and exhibits a relatively broad host range. Furthermore, it has a short latent period of approximately 10 min and a large burst size of about 110 PFU/cell at a multiplicity of infection (MOI) of 0.01, and it could effectively disrupt the biofilms formed by E. faecalis. Thus, this study provides a detailed characterization of E. faecalis phage SFQ1, which has great potential for treating E. faecalis infections.

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“…These phages exhibit a commendable ability to target and disrupt E. faecalis biofilms, paving the way for potential applications in treating biofilm-related infections and dental root canal treatments [17][18][19][20]. The efficacy demonstrated in inhibiting biofilm formation and eradicating planktonic E. faecalis cultures attests to the promising role of these phages in clinical scenarios [12,20,21].…”

Section: Introductionmentioning

confidence: 99%

“…The efficacy demonstrated in inhibiting biofilm formation and eradicating planktonic E . faecalis cultures attests to the promising role of these phages in clinical scenarios [ 12 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Insights into the novel Enterococcus faecalis phage: A comprehensive genome analysis

Abed,

Sholeh,

Khazani Asforooshani

et al. 2024

PLoS ONE

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Enterococcus faecalis, a Gram-positive bacterium, poses a significant clinical challenge owing to its intrinsic resistance to a broad spectrum of antibiotics, warranting urgent exploration of innovative therapeutic strategies. This study investigated the viability of phage therapy as an alternative intervention for antibiotic-resistant E. faecalis, with a specific emphasis on the comprehensive genomic analysis of bacteriophage SAM-E.f 12. The investigation involved whole-genome sequencing of SAM-E.f 12 using Illumina technology, resulting in a robust dataset for detailed genomic characterization. Bioinformatics analyses were employed to predict genes and assign functional annotations. The bacteriophage SAM-E.f 12, which belongs to the Siphoviridae family, exhibited substantial potential, with a burst size of 5.7 PFU/infected cells and a latent period of 20 min. Host range determination experiments demonstrated its effectiveness against clinical E. faecalis strains, positioning SAM-E.f 12 as a precise therapeutic agent. Stability assays underscore resilience across diverse environmental conditions. This study provides a comprehensive understanding of SAM-E.f 12 genomic composition, lytic lifecycle parameters, and practical applications, particularly its efficacy in murine wound models. These results emphasize the promising role of phage therapy, specifically its targeted approach against antibiotic-resistant E. faecalis strains. The nuanced insights derived from this research will contribute to the ongoing pursuit of efficacious phage therapies and offer valuable implications for addressing the clinical challenges associated with E. faecalis infections.

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Anti-biofilm Activity of a Lytic Phage Against Vancomycin-Resistant Enterococcus faecalis

Cited by 9 publications

References 40 publications

How Do Phages Disrupt the Structure of Enterococcus faecalis Biofilm?

How Do Phages Disrupt the Structure of Enterococcus faecalis Biofilm?

Characterization of an Enterococcus faecalis bacteriophage SFQ1 as a potential therapeutic agent

Insights into the novel Enterococcus faecalis phage: A comprehensive genome analysis

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