Anti-biofilm activity of the Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125

Papa, Rosanna; Parrilli, Ermenegilda; Sannino, Filomena; Barbato, Gaetano; Tutino, Maria Luisa; Artini, Marco; Selan, Laura

doi:10.1016/j.resmic.2013.01.010

Cited by 61 publications

(59 citation statements)

References 41 publications

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“…15 In this paper we confirmed this hypothesis by surface coating assay, the reported result demonstrated that P. haloplanktis TAC125 supernatant did not modify the surface properties of abiotic substrate.…”

Section: Discussionsupporting

confidence: 74%

“…13 Marine bacteria from Antarctica represent an untapped reservoir of biodiversity; indeed, Antarctic microorganisms can synthesize a broad range of potentially valuable bioactive compounds. [14][15][16] The present authors previously reported that P. haloplanktis TAC125 strain holds an anti-biofilm activity; 15 this bacterium had been isolated from Antarctic sea water near Terre Adelie. 17 The antibiofilm activity of cell-free supernatant of P. haloplanktis grown in static and in planktonic condition was tested on different staphylococci.…”

mentioning

confidence: 81%

“…A preliminary chemical characterization of the biofilm-inhibiting compound suggests that the biologically active component could be a polysaccharide. 15 The aim of the present work is to further investigate the chemical nature of P. haloplanktis TAC125 anti-biofilm molecules to understand the mode of action of active molecules.…”

mentioning

confidence: 99%

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Anti-biofilm activity of pseudoalteromonas haloplanktis tac125 against staphylococcus epidermidis biofilm: Evidence of a signal molecule involvement?

Parrilli

Papa²,

Carillo

et al. 2015

Int J Immunopathol Pharmacol

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Staphylococcus epidermidis is recognized as cause of biofilm-associated infections and interest in the development of new approaches for S. epidermidis biofilm treatment has increased. In a previous paper we reported that the supernatant of Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 presents an anti-biofilm activity against S. epidermidis and preliminary physico-chemical characterization of the supernatant suggested that this activity is due to a polysaccharide. In this work we further investigated the chemical nature of the anti-biofilm P. haloplanktis TAC125 molecule. The production of the molecule was evaluated in different conditions, and reported data demonstrated that it is produced in all P. haloplanktis TAC125 biofilm growth stages, also in minimal medium and at different temperatures. By using a surface coating assay, the surfactant nature of the anti-biofilm compound was excluded. Moreover, a purification procedure was set up and the analysis of an enriched fraction demonstrated that the anti-biofilm activity is not due to a polysaccharide molecule but that it is due to small hydrophobic molecules that likely work as signal. The enriched fraction was also used to evaluate the effect on S. epidermidis biofilm formation in dynamic condition by BioFlux system.

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Section: Discussionsupporting

confidence: 74%

mentioning

confidence: 81%

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Anti-biofilm activity of pseudoalteromonas haloplanktis tac125 against staphylococcus epidermidis biofilm: Evidence of a signal molecule involvement?

Parrilli

Papa²,

Carillo

et al. 2015

Int J Immunopathol Pharmacol

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“…Similarly recent reports revealed that, supernatant from Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125 significantly inhibited the biofilm formation in Staphylococcus epidermidis and Staphylococcus aureus [5]. An amino acid antibiotic known as Leucine 2-(hydroxymethoxyphosphinyl)-2-methylhydrazide, a phospholipase A2 inhibitor called as 4-Hydroxy-5-(hydroxymethyl)-3-(14-…”

Section: Introductionmentioning

confidence: 84%

Purification and antibiofilm activity of AHL-lactonase from endophytic Enterobacter aerogenes VT66

Rajesh

Ravishankar

2015

International Journal of Biological Macromolecules

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“…The search for QSI compounds has since turned to the ocean and its abundant variety of organisms, many of which are already known to produce useful bioactive compounds, such as halogenated furanones produced by the red alga D. pulchra (Rasmussen et al ., 2000), brominated alkaloids from Flustra foliacea (Peters et al ., 2003), and kojic acid from E. knighti (Dobretsov et al ., 2011). More recently, the culture supernatant from Antarctic marine bacterium P. haloplanktis TAC125 was shown to impair the formation of S. epidermidis biofilm (Papa et al ., 2013; Parrilli et al ., 2015). These findings demonstrate that QSI compounds are valuable antifouling agents in that they could serve as potential effective additives to environmentally friendly commercial antifouling paints due to their non‐toxicity (Tello et al ., 2012).…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm activity substances derived from coral symbiotic bacterial extract inhibit biofouling by the model strainPseudomonas aeruginosaPAO1

Song

Cai

Lao

et al. 2018

Microbial Biotechnology

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SummaryThe mitigation of biofouling has received significant research attention, with particular focus on non‐toxic and sustainable strategies. Here, we investigated quorum sensing inhibitor (QSI) bacteria as a means of controlling biofouling in a laboratory‐scale system. Approximately, 200 strains were isolated from coral (Pocillopora damicornis) and screened for their ability to inhibit quorum sensing (QS). Approximately, 15% of the isolates exhibited QSI activity, and a typical coral symbiotic bacterium, H12‐Vibrio alginolyticus, was selected in order for us to investigate quorum sensing inhibitory activity further. Confocal microscopy revealed that V. alginolyticus extract inhibited biofilm formation from Pseudomonas aeruginosa PAO1. In addition, the secondary metabolites of V. alginolyticus inhibited PAO1 virulence phenotypes by downregulating motility ability, elastase activity and rhamnolipid production. NMR and MS spectrometry suggested that the potential bioactive compound involved was rhodamine isothiocyanate. Quantitative real‐time PCR indicated that the bacterial extract induced a significant downregulation of QS regulatory genes (lasB, lasI, lasR, rhlI, rhlR) and virulence‐related genes (pqsA, pqsR). The possible mechanism underlying the action of rhodamine isothiocyanate analogue involves the disruption of the las and/or rhl system of PAO1. Our results highlight coral microbes as a bioresource pool for developing QS inhibitors and identifying novel antifouling agents.

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Anti-biofilm activity of the Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125

Cited by 61 publications

References 41 publications

Anti-biofilm activity of pseudoalteromonas haloplanktis tac125 against staphylococcus epidermidis biofilm: Evidence of a signal molecule involvement?

Anti-biofilm activity of pseudoalteromonas haloplanktis tac125 against staphylococcus epidermidis biofilm: Evidence of a signal molecule involvement?

Purification and antibiofilm activity of AHL-lactonase from endophytic Enterobacter aerogenes VT66

Antibiofilm activity substances derived from coral symbiotic bacterial extract inhibit biofouling by the model strainPseudomonas aeruginosaPAO1

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