Anti-cancer Activity and Gene Expression Responses to Methanol Extract of Gladiolus segetum in THP-1 Human Monocytic Leukemia Cells

Abstract

Objective: To Evaluate the viability of cells against exposure to the Methanol Extract of Gladiolus segetum (MEGS) by various colorimetric detection tests (Alamar Blue, WST-1) and the trypan blue exclusion test in Human Leukemia Monocytes THP-1 and Using reverse transcription-polymerase chain reaction assays. Methods: Human THP-1 monocytic cell line was exposed to 25 to 800 μg/mL concentrations of MEGS for 24 h and cellular viability was estimated using Alamar Blue, WST-1 and trypan blue tests. Gene expressions were performed using reverse transcription-polymerase chain reaction assays and the measurement of Caspase-3 enzyme activity. Results: A decrease of viability was observed with a dose-dependent effect of MEGS on THP-1. In addition, a differential expression involved in the different genes tested. Moreover, it has been shown overexpression of casp3 for an exposure to 25 μg/ml and 100 μg/ml of MEGS. Conclusion: Our results show that the MEGS present a toxicity to THP-1 cells, especially with regard to apoptosis processes.This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

Understanding the Link between Inflammasome and Apoptosis through the Response of THP-1 Cells against Drugs Using Droplet-Based Microfluidics

“…As stated previously, CASP-3 has an increasing level within the cells under inflammation conditions and there are several studies in the literature that promote this idea. According to these studies, it has been proven that inflammasome functions via oligomerization of the ASC, which is necessary for caspase-1 activation, release of IL-1β, induction of caspase-3 activation, and apoptotic cell death to occur eventually when a stimulant is present in the environment. − Thus, the decrease in cell fluorescence intensity toward the end of the experiment may be due to CASP-3 resulting in cell death.…”