Objective: The aim of the present study was to screen the anticancer activity of Atractylis flava Desf in butanolic extract (AFBE). In addition, we attempted to investigate the gene expression levels of seven relevant genes involved in the pathways leading to toxicity: oxidative stress (NCF1, OPA1, SDHA), inflammation (TNFα), apoptotic balance (PDCD4, BCL2, CASP8) and the measurement of caspase-3 activity on the acute monocytic leukemia cell line THP-1. Material and Methods: The cell viability was assessed using Trypan blue exclusion, alamarBlue® and WST-1 assays. The gene expression levels were tested by RT-qPCR. Activity of caspase-3 activity was performed using the EnzChek® Caspase-3 Assay kit to confirm the induction of apoptosis on tumor cells. Results: We observed significant growth inhibitory activity of the AFBE extract on the acute monocytic leukemia cell line THP-1. Moreover, the gene expression analysis showed that the plant extract caused statistically significant downregulation of selected genes compared to the untreated cells. Caspase-3 activity in the treated cells was significantly elevated. Conclusion: The presents study states that the butanolic extract of Atractylis flava Desf showed a significant cytotoxic effect against THP-1 cells. Our results suggest that the AFBE may be beneficial for the treatment and prevention of cancer.

Objective: To Evaluate the viability of cells against exposure to the Methanol Extract of Gladiolus segetum (MEGS) by various colorimetric detection tests (Alamar Blue, WST-1) and the trypan blue exclusion test in Human Leukemia Monocytes THP-1 and Using reverse transcription-polymerase chain reaction assays. Methods: Human THP-1 monocytic cell line was exposed to 25 to 800 μg/mL concentrations of MEGS for 24 h and cellular viability was estimated using Alamar Blue, WST-1 and trypan blue tests. Gene expressions were performed using reverse transcription-polymerase chain reaction assays and the measurement of Caspase-3 enzyme activity. Results: A decrease of viability was observed with a dose-dependent effect of MEGS on THP-1. In addition, a differential expression involved in the different genes tested. Moreover, it has been shown overexpression of casp3 for an exposure to 25 μg/ml and 100 μg/ml of MEGS. Conclusion: Our results show that the MEGS present a toxicity to THP-1 cells, especially with regard to apoptosis processes.This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.