Anti-Cancer and Anti-Proliferation Activity of Ethyl Asetat Extract From Ant Nest (Myrmecodia pendans) in Burkitt’s Lymphoma Cancer Cells

Abstract

Objective: To determine the activity of anti-cancer and anti-proliferation of ethyl acetate fraction of ant nest plants (Myrmecodia pendans) in Burkitt's Lymphoma cancer cells. Material and Methods: The study was conducted in a pure laboratory experimental method using Burkitt's Lymphoma cancer cell culture. Gradual research begins with the determination, extraction and fractionation of ant nest plants, to test for proliferation barriers. Data analysis using two-way ANOVA followed by Post Hoc LSD test with a significance level of 95%. Pearson correlation test was conducted. Results: The results of testing the inhibition of Burkitt's Lymphoma cell proliferation with ethyl acetate extract treatment showed that there was inhibition of cell growth based on the concentration given, starting from the lowest concentration of 15.625 µg/mL. Likewise, the incubation time factor of 24, 48, and 72 hours showed that the longer the incubation time, the greater the inhibition of cell growth. Antiproliferation analysis of flavonoid ethyl acetate extract based on concentration and incubation time on absorption of optical density Burkitt's Lymphoma was statistically significant (p = 0.00). Conclusion: Ant nest ethyl acetate extract has the effect of proliferation inhibition on Burkitt's lymphoma cells.

Cytotoxicity and Radiosensitizing Potentials of Pilosulin-3, a Recombinant Ant Venom, in Breast Cancer Cells

“…Notably, these values are lower than those reported for other ant venoms with anticancer effects. For instance, the study of fire ant ( Solenopsis invicta ) venom on endothelial cells reported an IC50 of 3 μg/mL [ 30 ], while the study of Achmad et al [ 31 ], testing the inhibition of Burkitt’s Lymphoma cell proliferation using ant nest ethyl acetate extract, reported inhibition of cell growth at the lowest concentration of 15.625 µg/mL. The IC50 of Bee ( A. mellifera ) venom on MDA-MB-231 was also higher (6.25 and 3.125 μg/mL) than that observed in the current study [ 32 ].…”

Medicinal Properties of Ant Nest Plant (Myrmecodia Genus): A Comprehensive Review

“…Many assays have been used to evaluate the toxicity activities of Myrmecodia species that associated with the potential of anticancer activity. Some crude extracts and compounds showed the inhibitory activity against various in vitro cancer cell lines (Table 2) [48], [49], [50], [51], [52], [53], [54], [55], [56], [57], [58]. Besides, Suharyanto and Purwono reported that M. pendens had effective anticancer activity in vivo model series.…”

Zerumbone induces growth inhibition of Burkitt’s lymphoma cell line via apoptosis

“…Burkitt's lymphoma is among most common cancers worldwide (Al-Attar et al, 1979;Achmad et al, 2019). Like all cancers, conventional treatment for lymphomas with immunotherapy and chemotherapy often lack efficacy due to the development of cancer resistance to the compounds.…”