Anti-cancer and antimicrobial potential of five soil Streptomycetes : a metabolomics-based study

Abstract

Lack of new anti-cancer and anti-infective agents directed the pharmaceutical research to natural products' discovery especially from actinomycetes as one of the major sources of bioactive compounds. Metabolomics- and dereplication-guided approach has been used successfully in chemical profiling of bioactive actinomycetes. We aimed to study the metabolomic profile of five bioactive actinomycetes to investigate the interesting metabolites responsible for their antimicrobial and anti-cancer activities. Three actinomycetes, namely, Streptomyces sp. SH8, SH10 and SH13, were found to exhibit broad spectrum of antimicrobial activities, whereas isolate SH4 showed the broadest antimicrobial activity against all tested strains. In addition, isolates SH8, SH10 and SH12 displayed potent cytotoxicity against the breast cancer cell line Michigan Cancer Foundation-7 (MCF-7), whereas isolates SH4 and SH12 exhibited potent anti-cancer activity against the hepatoma cell line hepatoma G2 (HepG2) compared with their weak inhibitory properties on the normal breast cells MCF-10A and normal liver cells transformed human liver epithelial-2 (THLE2), respectively. All bioactive isolates were molecularly identified as Streptomyces sp. via 16S rRNA gene sequencing. Our actinobacterial dereplication analysis revealed putative identification of several bioactive metabolites including tetracycline, oxytetracycline and a macrolide antibiotic, novamethymycin. Together, chemical profiling of bioactive Streptomycetes via dereplication and metabolomics helped in assigning their unique metabolites and predicting the bioactive compounds instigating their diverse bioactivities.

Antiulcer Potential of Psidium guajava Seed Extract Supported by Metabolic Profiling and Molecular Docking

“…One mg of the sample was injected into the BEH C18 column, at 40 °C, and connected to the guard column. A gradient elution was used, starting from no solvent A and 0.1% formic acid in water to 100% acetonitrile as solvent B. MZmine 2.12 San Diego, USA was used to investigate MS data using mzML format converted with ProteoWizard Palo Alto, CA, USA [ 27 ].…”

Chemical diversity, medicinal potentialities, biosynthesis, and pharmacokinetics of anthraquinones and their congeners derived from marine fungi: a comprehensive update

“…Throughout history, different natural sources have been used for treatment of diseases, and more recently as sources and valuable suppliers of biologically active compounds with diverse bioactivities that can be developed to be used in new drugs. [1][2][3][4][5][6] Intriguingly, marine organisms and microorganisms were among the valuable sources of new natural products. 2 Microbial secondary metabolites have been known for their chemical diversity and a broad range of bioactivities.…”

“…2 Microbial secondary metabolites have been known for their chemical diversity and a broad range of bioactivities. 6,7 Marine microorganisms are considered highly productive sources of physiologically active compounds including peptides, polyketides, terpenes, and alkaloids. [8][9][10] Some marine-based compounds have been approved as drugs with different pharmacological uses, 11,12 while several others are under different clinical trials before their approval as new drugs.…”

Chemical Diversity and Biological Activities of Anthraquinones Derived from Marine Fungi: A Comprehensive Update

“…Throughout history, different natural sources have been used as valuable suppliers of biologically active compounds with diverse bioactivities that can be used to develop new drugs [1][2][3][4][5][6]. Marine organisms and microorganisms were among the valuable sources of new natural products [2].…”

“…Marine organisms and microorganisms were among the valuable sources of new natural products [2]. Microbial secondary metabolites have been known for their chemical diversity and broad range of bioactivities [6,7]. Marine microorganisms have been considered as highly productive sources of physiologically active compounds including peptides, anthraquinones, polyketides, terpenes, and alkaloids [8][9][10].…”