Anti-cancer Potential of Polysaccharide Extracted From Polygonatum sibiricum on HepG2 Cells via Cell Cycle Arrest and Apoptosis

Abstract

Polygonatum sibiricum is one of the most widely used traditional Chinese medicine in China. Polygonatum sibiricum polysaccharide (PSP) is the main functional component of Polygonatum sibiricum. In this study, a water-soluble polysaccharide (PSP-1) was first isolated from Polygonatum sibiricum with a molecular weight of 38.65 kDa. Structural analysis was performed via methylation and FT-IR spectroscopy analyses, which in combination with NMR spectroscopy, revealed that PSP-1 has a → 4-α-D-Glcp-1 → backbone with the substitution at O-6 with the β-D-Glcp-1 → residues. Furthermore, PSP-1 exhibited potent and concentration-dependent anticancer effects, inducing HepG2 cell apoptosis and arresting the cell cycle at the G1 phase. Moreover, PSP-1 also decreased the mitochondrial membrane potential, damaged the nucleus of HepG2 cells, and increased the activity of caspase-9 and−3 in the intrinsic apoptotic pathways to induce HepG2 cell apoptosis. To conclude, PSP-1 might be a good candidate for the treatment of liver cancer, and this work provides important information for understanding the relationship between structure and antitumor activity of PSP-1, which is relevant for the treatment of hepatocellular carcinoma in clinic.

Oral Supplementation of Dragon Power® Polygonatum had Beneficial Effects on Testosterone's Relationship with Cardiorespiratory Fitness and Erection: a Double-Blind, Placebo-Controlled Study

“…These results revealed that PCP had good anti-fatigue ability. Polygonatum sibiricum extract increased the activity of secondary messengers of cellular aerobic capacity, such as AMPK and p38 [30]. These secondary messengers stimulated the increase in the synthesis of cell mitochondria and antioxidant enzymes, which may have indirectly improved aerobic capacity [31].…”

Isolation, Purification, Fractionation, and Hepatoprotective Activity of Polygonatum Polysaccharides

“…HepG2 cells were cultured in 1640 medium supplemented with 10% fetal bovine serum in a CO 2 incubator set at 37 °C, saturated humidity, and 5% CO 2 [ 38 ].…”

Health-Promoting Activities and Associated Mechanisms of Polygonati Rhizoma Polysaccharides

“…PREPS may show long-lasting anti-tumor effects in assisting TNBC therapies through sustaining hematopoiesis and lymphoid regeneration in bone marrow [ 130 ]. In a HepG2 cell, the water-soluble PSP (38.65 kDa) showed concentration-dependent anticancer effects through arresting the cell cycle at the G1 phase, decreasing mitochondrial membrane potential, damaging the nucleus, and inducing cell apoptosis via increasing the activity of caspase-9 and caspase-3 [ 131 ].…”