Anti-chicken type I IFN countermeasures by major avian RNA viruses

Anjum, Faisal Rasheed; Anam, Sidra; Rahman, Sajjad Ur; Ali, Sultan; Aslam, Muhammad; Rizvi, Farzana; Asif, Muhammad; Abdullah, Rana Muhammad; Abaidullah, Muhammad; Shakir, Muhammad Zulqarnain; Goraya, Mohsan Ullah

doi:10.1016/j.virusres.2020.198061

Virus Research

2020

DOI: 10.1016/j.virusres.2020.198061

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Anti-chicken type I IFN countermeasures by major avian RNA viruses

Faisal Rasheed Anjum

Sidra Anam

Sajjad Ur Rahman

et al.

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Cited by 4 publications

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“…Type I IFNs are an integral part of the chicken's innate immune mechanisms and provide the first line of innate immune protection against a variety of viruses (Anjum et al 2020b). To date, three sub-Type I interferon (IFN) was first discovered by Isaacs and Lindenmann during the cultivation of the avian influenza virus (AIV) in embryonated chicken eggs (Isaacs and Lindenmann 1957).…”

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confidence: 99%

Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells

Anjum¹,

Rahman²,

Aslam³

et al. 2021

Vet. Med. - Czech

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Chicken interferon-α (chIFN-α) is an important antiviral cytokine and represents one of the first lines of the chicken’s innate immune system. The current study is the first-ever report of chicken IFN (chIFN) production in Pakistan. In this study, we have used live and UV-irradiated Newcastle disease virus (NDV) to induce the expression of chIFN-α in chicken embryo fibroblast (CEF) cells. ChIFN-α was partially purified in a two-step protocol; ultracentrifugation followed by treatment with anti-chIFN-β antibodies. The purified chIFN-α was ana-lysed via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the in vitro antiviral potential of chIFN-α was determined against the H9N2 avian influenza virus (AIV) via a cytopathic inhibition assay. The relative mRNA level of the IFN-stimulated genes (ISGs) in the IFN-stimulated CEF cells was measured at various time intervals by a quantitative polymerase chain reaction (qPCR). The stability of natural chIFN-α to the temperature, pH, and ultraviolet (UV) light was also determined. The in vivo therapeutic potential of chIFN-α was determined in 7-day-old broiler chickens challenged with AIV. We found that a higher chIFN-α expression level was induced by the UV-irradiated NDV in the CEF cells as compared to the live NDV. The UV-irradiated NDV induced the maximum IFN production in the CEF cells at 24 h post-infection. Two bands of 21 kDa on SDS-PAGE confirmed the presence of the chIFN-α protein. The cytopathic inhibition assay indicated the strong antiviral activity of chIFN-α against AIV. Our results of the stability analysis showed that chIFN-α was stable at a wide range of temperatures and pH levels. However, a little exposure to UV-light resulted in a significant loss of antiviral activity. We also observed that the antiviral activity of chIFN-α is related to the expression levels of the antiviral ISGs. The results of the in vivo study showed that the chIFN-α therapy via the oral route resulted in a significant improvement in the tracheal pathology of chickens challenged with AIV. In conclusion, we suggest that chIFN-α could be an important therapeutic tool to control avian influenza infection in poultry.

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confidence: 99%

Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells

Anjum¹,

Rahman²,

Aslam³

et al. 2021

Vet. Med. - Czech

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Proteomic analysis of EPC cell replication effect of Hsp70 on SVCV infection at different temperatures

et al. 2023

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Immunological studies on chicken interferon-kappa using an antigen-capture ELISA developed using new mouse monoclonal antibodies

Lee

Lillehoj

2021

Developmental & Comparative Immunology

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Anti-chicken type I IFN countermeasures by major avian RNA viruses

Cited by 4 publications

References 91 publications

Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells

Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells

Proteomic analysis of EPC cell replication effect of Hsp70 on SVCV infection at different temperatures

Immunological studies on chicken interferon-kappa using an antigen-capture ELISA developed using new mouse monoclonal antibodies

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Anti-chicken type I IFN countermeasures by major avian RNA viruses

Cited by 4 publications

References 91 publications

Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells

Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells

Proteomic analysis of EPC cell replication effect of Hsp70 on SVCV infection at different temperatures

Immunological studies on chicken interferon-kappa using an antigen-capture ELISA developed using new mouse monoclonal antibodies

Contact Info

Product

Resources

About

Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells

Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells