Anti-complementary activity of protostane-type triterpenes fromAlismatis rhizoma

Abstract

Four protostane-type triterpenes, alisol B 23-acetate (1a), alisol C 23-acetate (2a), alisol B (3a), and alisol A 24-acetate (4a), were isolated from the rhizome of Alismatis plantago-aquatice L. var. orientale Samuelson (Alismataceae) and eleven protostane derivatives (compounds 1-11) were obtained by selective modification from alisol B 23-acetate (1a). These compounds were investigated for their anti-complement activity against the classical pathway of the complement system. Alisol B (3a) and alisol A 24-acetate (4a) exhibited anti-complement activity with IC50 values of 150 and 130 microM. Among the synthetic derivatives, the tetrahydroxylated protostane triterpene (9) showed moderate inhibitory activity with IC50 value of 97.1 microM. Introduction of an aldehyde group at C-23 (10; IC50 value, 47.7 microM) showed the most potent inhibitory effect on the complement system in vitro.

Isolation and characterization of an anti-complementary polysaccharide D3-S1 from the roots of Bupleurum smithii

“…The best described anti-complementary agents are polysaccharides, flavones and triterpenes [27][28][29]. But most of the targets of these agents in the complement activation cascade were not fully elucidated due to lack of the efficient method to purify the active ingredients to homogeneity from crude extract and the expensive of the complementdeficient serum.…”

Isolation and characterization of an anti-complementary protein-bound polysaccharide from the stem barks of Eucommia ulmoides

“…The best described anticomplementary agents are polysaccharides, flavones and triterpenes [43][44][45]. But most of the targets of these agents in the complement activation cascade were not fully elucidated due to lack of the efficient method to purify the active ingredients to homogeneity from crude extract and the expensive of the complement deficient serum [39].…”

Alisol B acetate induces apoptosis of SGC7901 cells via mitochondrial and phosphatidylinositol 3-kinases/Akt signaling pathways

“…Alisol B acetate is a major ingredient isolated from Alismatis rhizoma and has been used for urological diseases in traditional Chinese medicine. In recent years, the pharmacological characterization of alisol B acetate has been identified and several biological activities have been defined, such as the inhibitory effects on lipopolysaccharide [3] , the inhibition of complementary activity [4,5] and antibodymediated allergic reaction [6] . Furthermore, it has been demonstrated that alisol B acetate induces cell death in hepatoma and leukemia cells [7,8] .…”