Anti-diabetic activity of different oils through their effect on arylsulfatases

Abstract

BackgroundDiabetes mellitus (DM) is characterized by the overproduction of the reactive oxygen species which affects the integrity of the lysosomal membrane affecting lysosomal enzymes. The effect of these species is blocked by some natural products as antioxidants. In the current study, groups of normal and streptozotocin (STZ)-induced diabetic rats were treated by Nigella sativa (NS), olive and canola oils and subjected to the study of arylsulfatases as a model of lysosomal enzymes. The aim of the present study is to investigate the effect of STZ-induced diabetes on arylsulfatases in presence and absence of NS, olive and canola oils.MethodsDifferent groups of rats were induced by STZ, treated with different oils and compared to their corresponding control group. All groups were subjected for the assays of blood glucose, insulin, catalase and arylsulfatases. A comparative kinetic study of arylsulfatses was performed to detect the alteration of catalytic characterization.ResultsThe results demonstrated that diabetes causes a significant elevation in the level of hepatic arylsulfatase B and a significant reduction of hepatic catalase as an antioxidant enzyme. NS and olive oils returned catalase and arylsulfatase B activities back near to normal by fixing their catalytic properties. Furthermore, the maximum velocity of arylsulfatases A and B was significantly elevated in the induced diabetes, whereas their Km values were significantly changed. The treatment of diabetic rats by NS and olive oils reduced the degree of significance.ConclusionDiabetes induces significant alterations of the catalytic characters of arylsulfatases and some oils decrease this alteration through an antioxidant-mediated effect.

Thymoquinone ameliorates some endocrine parameters and histological alteration in a rat model of polycystic ovary syndrome

“…Some studies revealed that the extract of N. sativa seeds increases glucose-induced insulin release in isolated Langerhans islets from rats ( 16 ). Some other studies also have suggested anti-diabetic effects of N. sativa and thymoquinone ( 35 , 36 ).…”

Critical Review on the Significance of Olive Phytochemicals in Plant Physiology and Human Health

“…Olive oils have demonstrated, in animal studies, a strong capacity to improve blood glucose concentration and diabetic complications at the enzymatic level, their mechanism of action being assigned to their antioxidant activity [ 170 ]. Moreover, this fact has been supported by the capacity of bioactive compounds of olive oil to inhibit the gluconeogenesis pathways in liver, as a result of the inhibition of glucose-6-phosphatese enzyme, besides the capacity to induce a significant enhancement in the hepatic catalase activity in vivo [ 200 ]. In the same way, Al-Azzawie and Alhamdani [ 162 ] demonstrated the capacity of the long-term intake (16 weeks) of oleuropein to restore glucose blood levels in diabetic rabbits [ 162 ].…”

Zinc and curcumin lower arylsulfatses and some metabolic parameters in streptozotocin-induced diabetes

“…The disturbances of its homeostasis seem to be associated not only with diabetes, but also with others [ 4 ]. Recently, it was reported that the lysosomal enzymes arylsulfatases were significantly changed in experimental diabetes [ 5 ]. In fact, the treatment of diabetes through food sources is valuable around the world.…”