Anti‐DNA topoisomerase IIα autoantibodies in localized scleroderma

Abstract

Objective. To determine the prevalence and clinical correlation of anti-DNA topoisomerase II␣ (antitopo II␣) antibody in patients with localized scleroderma.Methods. Anti-topo II␣ antibodies or anti-DNA topoisomerase I (topo I) antibodies were determined by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Inhibition of topo II␣ enzymatic activity by the antibodies was evaluated by decatenation assays using kinetoplast DNA as a substrate. Results. IgG or IgM anti-topo II␣ antibody was detected in 76% ( Conclusion.The results of the present study indicate that anti-topo II␣ is a major autoantibody in localized scleroderma, and is distinct from anti-topo I antibody in SSc.

Autoantibodies against matrix metalloproteinase-1 in patients with localized scleroderma

“…Especially, antihistone Ab correlates with total number of lesions and the total area of lesions [2]. On the other hand, autoantibodies against SOD or DNA topoisomerase II are found at comparable frequency between the 3 subgroups of LSc [8,9]. By contrast, the present study showed that anti-MMP-1 Ab was most frequently detected in morphea (60%), followed by linear scleroderma (47%) and then generalized morphea (25%).…”

“…However, these autoantibodies specific to SSc are absent in LSc with few exceptions [4]. Conversely, autoantibodies against DNA topoisomerase II and SOD, which are detected in LSc, are not found in SSc [8,9]. Thus, although both SSc and LSc show cutaneous fibrosis, autoantibody specificities associated with these diseases are different.…”

“…These autoantibodies react to various intracellular components, including nucleosome, histone, DNA topoisomerase II, and single-stranded DNA (ssDNA) [3,4,[6][7][8] and also to some enzymes, such as superoxide dismutase (SOD) [9]. In addition, anti-cardiolipin Ab, rheumatoid factor, and lupus erythematosus cell phenomenon have been detected in LSc [4,10].…”

Diagnosis and treatment of systemic and localized scleroderma

“…In another study, a high prevalence of topoisomerase II was found in patients with localized scleroderma, distinct from topoisomerase I seen in patients with systemic sclerosis [59].…”

Vitiligo, morphea and Sjögren’s syndrome: a case study

“…In addition to positive titers of ANA, antidsDNA, and anti-scleroderma (-Scl) 70 antibodies, increased RF levels suggest that an immunological process occurring in morphea. 6,7 Although Sjögren's syndrome, vitiligo, and morphea are autoimmune diseases, they are seen together rarely. A few studies have report- ed an association between morphea and vitiligo to date.…”