Anti‐elastase and anti‐hyaluronidase of phenolic substance from Areca catechu as a new anti‐ageing agent

Abstract

We have previously screened 150 medicinal plants for the inhibition of elastase and found significant inhibitory effects of the extracts of Areca catechu L. on the ageing and inflammation of skin tissues. To isolate and identify the compounds having biological activity, they were further purified by each fraction of solvents, silica gel column chromatography, preparative TLC and reversed-phase HPLC. The peak in HPLC, which coincided with the inhibitory activity against elastase, was identified as a phenolic substance by using various colorimetric methods, UV and IR. IC(50) values of this phenolic substance were 26.9 mug mL(-1) for porcine pancreatic elastase (PPE) and 60.8 mug mL(-1) for human neutrophil elastase (HNE). This phenolic substance showed more potent activity than that of reference compounds, oleanolic acid (76.5 mug mL(-1) for PPE, 219.2 mug mL(-1) for HNE) and ursolic acid (31.0 mug mL(-1) for PPE, 118.6 mug mL(-1) for HNE). According to the Lineweaver-Burk plots, the inhibition against both PPE and HNE by this phenolic substance was competitive inhibition with the substrate. The phenolic substance from A. catechu effectively inhibited hyaluronidase activity (IC(50) : 210 mug mL(-1) ). These results suggest that the phenolic substance purified from A. catechu has an anti-ageing effect by protecting connective tissue proteins.

Matrix metalloproteinase, hyaluronidase and elastase inhibitory potential of standardized extract ofCentella asiatica

“…After standing at room temperature for 10 min, the absorbance of the reaction mixture was measured at 600 nm. The performance of the assay was verified using ursolic acid as a reference (Lee et al, 2001) under exactly the same experimental conditions. A 600 nm value of intact undigested hyaluronic acid was set at 100%.…”

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“…These results suggest that phenolic substance purified from A. catechu has an anti-ageing effect by protecting connective tissue 15 .…”

Correlating the Anti – Aging Activity with the Bioactive Profile of Chlorella emersonii KJ725233; Its Toxicological Studies for a Potential use in Cosmeceuticals

“…Epigallocatechin was assayed as a positive control. Anti -Hyaluronidase activity was spectrophotometrically determined by measuring the amount of N-acetyl glucosamine produced by the action of bovine hyaluronidase on sodium hyaluronate as earlier reported by Lee et al 19 Bovine hyaluronidase was added to varying concentrations of the extract (100 µg/mL, 500 µg/mL, 1000 µg/mL) and incubated at 37 o C for 20 min. Hyaluronidase was activated by adding 12.5 mM calcium chloride to the reaction mixture and was further incubated at 37 o C for 20 min.…”