Anti-fatigue effect of fermented porcine placenta through the regulation of fatigue-associated inflammatory cytokines

Nam, Sun‐Young; Go, Ji-Hyun; Lee, Mi-Kyung; Kim, Jong-Bae; Jeong, Hyein; Lee, Won Kyung

doi:10.5667/tang.2016.0011

Cited by 3 publications

(3 citation statements)

References 28 publications

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“…Gly-Leu and Leu-Gly dipeptides of fermented porcine placenta extract have been reported to have a reducing effect on fatigue ( Nam et al ., 2016 ). However, no studies have analyzed the effects of dipeptides on skin.…”

Section: Discussionmentioning

confidence: 99%

Oral Administration of Glycine and Leucine Dipeptides Improves Skin Hydration and Elasticity in UVB-Irradiated Hairless Mice

Park

Lee

Shin

et al. 2017

Biomolecules & Therapeutics

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Placenta is a special organ that contains many nutrients such as growth factors, minerals, and bioactive peptides. Dipeptides of glycine and leucine are major components of porcine placenta extracts (PPE) that has been used as an alternative of human placenta extracts. In this study, we investigated whether major peptides of PPE, Glycyl-L-Leucine (Gly-Leu), L-Leucyl-Glycine (Leu-Gly), and L-Leucyl-L-Leucine (Leu-Leu), affect skin hydration and elasticity in vitro and in vivo. We found that Gly-Leu and Leu-Gly dipeptides induced the expression of transglutaminase 1 in normal human epidermal keratinocytes (NHEKs) whereas Leu-Leu dipeptides did not. Treatment with Gly-Leu or Leu-Gly significantly increased hyaluronan (HA) synthesis in NHEKs and the upregulation of hyaluronan synthase 2 (HAS2) mRNA level was confirmed. In addition, elastase activity was inhibited in NHEKs treated with Gly-Leu or Leu-Gly dipeptides. Oral administration of Gly-Leu or Leu-Gly dipeptides increased skin hydration and elasticity in UVB-irradiated hairless mice. The significant upregulation of HA in UVB-irradiated hairless mice was observed in response to oral administration of Gly-Leu or Leu-Gly. These results suggest that the major dipeptides of porcine placenta, Gly-Leu and Leu-Gly, are potentially active ingredients for skin moisturization formulations.

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Section: Discussionmentioning

confidence: 99%

Oral Administration of Glycine and Leucine Dipeptides Improves Skin Hydration and Elasticity in UVB-Irradiated Hairless Mice

Park

Lee

Shin

et al. 2017

Biomolecules & Therapeutics

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“…HMC‐1 cells (3 × 10 6 ) were pre‐treated with NG for 2 hours and then treated with TSLP (20 ng/mL) for 8 hours to determine pSTAT6 and MDM2 levels or 48 hours to determine PARP and p53 levels. Western blot analysis was performed as previously described . Briefly, NG‐treated HMC‐1 cells were lysed.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory effect of naringenin via IL‐13 level regulation on thymic stromal lymphopoietin‐induced inflammatory reactions

Han

Moon

Ryu

et al. 2017

Clin Exp Pharma Physio

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Naringenin (NG) has various beneficial properties, such as anti-cancer and anti-inflammatory effects. Thymic stromal lymphopoietin (TSLP) induces mast cell proliferation and inflammatory reactions. The aim of this study was to investigate the regulatory effect of NG on TSLP-induced mast cell proliferation and inflammatory reactions using human mast cell line (HMC-1) cells. HMC-1 cells were pre-treated with NG and then treated with TSLP. HMC-1 cells proliferation was determined by quantifying bromodeoxyuridine incorporation. Levels of anti-apoptotic and pro-apoptotic factors were analyzed by western blot analysis. The productions and mRNA expressions of interleukin (IL)-13 and tumour necrosis factor-α (TNF-α) were analyzed by ELISA and quantitative real-time PCR. We found that NG significantly attenuated HMC-1 cells proliferation and Ki-67 mRNA expression promoted by TSLP. NG significantly suppressed mRNA expression of TSLP receptor and IL-7 receptor α in TSLP-treated HMC-1 cells. NG significantly down-regulated levels of phosphorylated-signal transducer and activation of transcription 6 and murine double-minute 2 in TSLP-treated HMC-1 cells, up-regulated levels of cleaved poly ADP-ribose polymerase and p53 in TSLP-treated HMC-1 cells. Furthermore, NG significantly decreased the productions and mRNA expressions of IL-13 and TNF-α in TSLP-treated HMC-1 cells. These results suggest NG has an inhibitory effect on mast cell-mediated allergic inflammatory reactions.

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“…HMC‐1 (1 × 10 4 ) was cultured for 2 hr in the presence of PEITC (0.04, 0.4, and 4 μM) and then stimulated with TSLP (20 ng/mL) for 48 hr. The MTT assay was carried out as previously described (Nam et al, ).…”

Section: Methodsmentioning

confidence: 99%

Phenethyl isothiocyanate decreases thymic stromal lymphopoietin-induced inflammatory reactions in mast cells

et al. 2017

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Phenethyl isothiocyanate (PEITC) has been reported to have anti‐inflammatory and anti‐carcinogenic properties. However, the anti‐inflammatory effects of PEITC on thymic stromal lymphopoietin (TSLP)‐induced inflammatory responses are uncertain. This study evaluates pharmacological activities of PEITC on inflammatory reactions in TSLP‐stimulated mast cells. Human mast cell line HMC‐1 was treated with PEITC (0.04, 0.4, and 4 µM) and subjected to inflammation by TSLP. Our results showed that PEITC significantly attenuated IL‐13 and TNF‐α levels increased by TSLP in HMC‐1. PEITC significantly decreased TSLP‐promoted HMC‐1 proliferation and Ki67 mRNA expression. Protein levels of MDM2 and pSTAT6 increased by TSLP were significantly suppressed by PEITC in HMC‐1. In addition, PEITC significantly enhanced protein levels of cleaved poly ADP‐ribose polymerase and p53 decreased by TSLP. Based on the effects of PEITC on inflammation and proliferation in this study, it is possible that PEITC is a potential candidate to treat mast cells‐mediated inflammatory disorders. Practical applications This report provides strong evidence that Phenethyl isothiocyanate (PEITC) which is a dietary constituent derived from cruciferous vegetables, may be considered an alternative agent for treatment of mast cells‐mediated inflammatory disorders.

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Anti-fatigue effect of fermented porcine placenta through the regulation of fatigue-associated inflammatory cytokines

Cited by 3 publications

References 28 publications

Oral Administration of Glycine and Leucine Dipeptides Improves Skin Hydration and Elasticity in UVB-Irradiated Hairless Mice

Oral Administration of Glycine and Leucine Dipeptides Improves Skin Hydration and Elasticity in UVB-Irradiated Hairless Mice

Inhibitory effect of naringenin via IL‐13 level regulation on thymic stromal lymphopoietin‐induced inflammatory reactions

Phenethyl isothiocyanate decreases thymic stromal lymphopoietin-induced inflammatory reactions in mast cells

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