Anti-fatigue effect of glycoprotein from hairtail (Trichiurus lepturus) by-products in a behavioral mouse model

Chen, Jiaqi; Lu, Xiaodan; Chen, Peixin; Shen, Yanjun; Zheng, Baodong; Guo, Zebin

doi:10.1016/j.fochx.2023.100645

Cited by 12 publications

(5 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, increasing levels of ATP, AMP, and muscle glycogen have been linked to improved endurance, while reducing the levels of MDA and SOD has been suggested to decrease fatigue and enhance fatigue resistance. Additionally, 5-HT is a major biochemical marker for assessing fatigue in different species, and its levels can be detected by ELISA and HPLC with electrochemical and fluorescence detection [34]. The results of this study showed that NBH extended swimming time in test mice, increased levels of LG and reduced levels of BUN, LDH, BA, and BLA, indicating its potential for decreasing physical fatigue and improving exercise capacity.…”

Section: Discussionmentioning

confidence: 75%

The Efficient Synthesis and Anti-Fatigue Activity Evaluation of Macamides: The Unique Bioactive Compounds in Maca

et al. 2023

View full text Add to dashboard Cite

Macamides are a class of amide alkaloids that are only found in maca and are widely considered to be its bioactive marker compounds. More than thirty macamide monomers have been identified in recent years; however, it is difficult to obtain a single macamide monomer from the maca plant because of their similar structures and characteristics. We used the carbodiimide condensation method (CCM) to efficiently synthesize five typical macamides, including N-benzyl-hexadecanamide (NBH), N-benzyl-9Z,12Z,15Z-octadecenamide, N-(3-methoxybenzyl)-9Z,12Z-octadecenamide, N-benzyl-9Z,12Z-octadecenamide, and N-(3-methoxybenzyl)-9Z,12Z,15Z-octadecadienamide. All the synthesized macamides were purified by a one-step HPLC with a purity of more than 95%. NBH is the most abundant macamide monomer in natural maca, and it was selected to evaluate the anti-fatigue effects of macamides. The results indicated that NBH could enhance the endurance capacity of mice by increasing liver glycogen levels and decreasing blood urea nitrogen, lactate dehydrogenase, blood ammonia, and blood lactic acid levels. Macamides might be the active substances that give maca its anti-fatigue active function.

show abstract

Section: Discussionmentioning

confidence: 75%

The Efficient Synthesis and Anti-Fatigue Activity Evaluation of Macamides: The Unique Bioactive Compounds in Maca

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Notably, the symptoms of fatigue are commonly found in patients with various diseases [ 37 , 38 ]. Recently, the role of functional foods in managing these symptoms has attracted the attention of research [ 39 , 40 ]. Our previous study demonstrated that FA treatment effectively improved the loss of muscle function and mass (sarcopenia) [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of HY7602 Fermented Deer Antler on Physical Fatigue and Antioxidant Activity in Mice

Jeon,

Lee,

Kim

et al. 2024

IJMS

View full text Add to dashboard Cite

Lactobacillus curvatus HY7602 fermented antler (FA) ameliorates sarcopenia and improves exercise performance by increasing muscle mass, muscle fiber regeneration, and mitochondrial biogenesis; however, its anti-fatigue and antioxidant effects have not been studied. Therefore, this study aimed to investigate the anti-fatigue and antioxidant effects and mechanisms of FA. C2C12 and HepG2 cells were stimulated with 1 mM of hydrogen peroxide (H2O2) to induce oxidative stress, followed by treatment with FA. Additionally, 44-week-old C57BL/6J mice were orally administered FA for 4 weeks. FA treatment (5–100 μg/mL) significantly attenuated H2O2-induced cytotoxicity and reactive oxygen species (ROS) production in both cell lines in a dose-dependent manner. In vivo experiments showed that FA treatment significantly increased the mobility time of mice in the forced swimming test and significantly downregulated the serum levels of alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatine kinase (CK), and lactate. Notably, FA treatment significantly upregulated the activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione/oxidized glutathione ratio (GSH/GSSG) and increased the mRNA expression of antioxidant genes (SOD1, SOD2, CAT, GPx1, GPx2, and GSR) in the liver. Conclusively, FA is a potentially useful functional food ingredient for improving fatigue through its antioxidant effects.

show abstract

“…All behavioral assessments were conducted in a noiseless room. For the passive avoidance test, the mice were placed in a shuttle box and stimulated by light (or sound) and electric shock to record the active avoidance response index during the establishment of this conditioned reflex, which can reflect the changes in learning and memory ability of experimental animals 31 , 32 . The passive avoidance test consists of a dark avoidance test and a shuttle experiment, with an acquisition trial and a retention test 24 h later.…”

Section: Methodsmentioning

confidence: 99%

mtDNA-cGAS-STING axis-dependent NLRP3 inflammasome activation contributes to postoperative cognitive dysfunction induced by sevoflurane in mice

Yang,

Zhong,

Sha

et al. 2024

Int. J. Biol. Sci.

View full text Add to dashboard Cite

The activation of NLRP3 inflammasome in microglia is critical for neuroinflammation during postoperative cognitive dysfunction (POCD) induced by sevoflurane. However, the molecular mechanism by which sevoflurane activates the NLRP3 inflammasome in microglia remains unclear. The cGAS-STING pathway is an evolutionarily conserved inflammatory defense mechanism. The role of the cGAS-STING pathway in sevoflurane-induced NLRP3 inflammasome-dependent neuroinflammation and the underlying mechanisms require further investigation. We found that prolonged anesthesia with sevoflurane induced cognitive dysfunction and triggered the neuroinflammation characterized by the activation of NLRP3 inflammasome in vivo . Interestingly, the cGAS-STING pathway was activated in the hippocampus of mice receiving sevoflurane. While the blockade of cGAS with RU.521 attenuated cognitive dysfunction and NLRP3 inflammasome activation in mice. In vitro , we found that sevoflurane treatment significantly activated the cGAS-STING pathway in microglia, while RU.521 pre-treatment robustly inhibited sevoflurane-induced NLRP3 inflammasome activation. Mechanistically, sevoflurane-induced mitochondrial fission in microglia and released mitochondrial DNA (mtDNA) into the cytoplasm, which could be abolished with Mdivi-1. Blocking the mtDNA release via the mPTP-VDAC channel inhibitor attenuated sevoflurane-induced mtDNA cytosolic escape and reduced cGAS-STING pathway activation in microglia, finally inhibiting the NLRP3 inflammasome activation. Therefore, regulating neuroinflammation by targeting the cGAS-STING pathway may provide a novel therapeutic target for POCD.

show abstract

Anti-fatigue effect of glycoprotein from hairtail (Trichiurus lepturus) by-products in a behavioral mouse model

Cited by 12 publications

References 40 publications

The Efficient Synthesis and Anti-Fatigue Activity Evaluation of Macamides: The Unique Bioactive Compounds in Maca

The Efficient Synthesis and Anti-Fatigue Activity Evaluation of Macamides: The Unique Bioactive Compounds in Maca

Effect of HY7602 Fermented Deer Antler on Physical Fatigue and Antioxidant Activity in Mice

mtDNA-cGAS-STING axis-dependent NLRP3 inflammasome activation contributes to postoperative cognitive dysfunction induced by sevoflurane in mice

Contact Info

Product

Resources

About