Anti-Fouling Chemistry of Chiral Monolayers: Enhancing Biofilm Resistance on Racemic Surface

Bandyopadhyay, Debjyoti; Prashar, Deepali; Luk, Yan Yeung

doi:10.1021/la200230t

Cited by 42 publications

(26 citation statements)

References 72 publications

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“…These groups include tri(propylene sulfoxide) (TPS) [99], Mannitol [100], gulitolor mannonamide [101], hyperbranched or dendritic polyglycerol [102], and oligopeptides [103]. None of these SAMs were tested for fouling from blood plasma or serum.…”

Section: Other Samsmentioning

confidence: 99%

Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications

2015

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This review focuses on recent advances in the development of functionalizable antifouling coatings and their applications in label-free optical biosensors. Approaches to the development of antifouling coatings, ranging from self-assembled monolayers and PEG derivatives to ultra-low-fouling polymer brushes, are reviewed. Methods of preparation and characterization of antifouling coatings and the functionalization of antifouling coatings with bioreceptors are reviewed, and the effect of functionalization on the fouling properties of biofunctional coating is discussed. Special attention is given to biofunctional coatings for label-free bioanalysis of blood plasma and serum for medical diagnostics.

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Section: Other Samsmentioning

confidence: 99%

Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications

2015

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“…Various bacteria are able to form biofilms on the surfaces of eukaryotes, including marine algae, diatoms, and sponges, to foul the inhabited surfaces (95). Thus, many marine organisms have evolved efficient strategies to combat bacterial overgrowth (e.g., the production of inhibitory compounds [96,97] that are either toxic to bacteria, such as antibiotics [98,99], or interfere with bacterial cell-cell communication to prevent the establishment of biofilms on surfaces [100,101]). Thus, bacteria associated with eukaryotes are attracting attention because they are remarkable candidates for rich sources of novel natural bioactive compounds for use in biotechnology or in novel potential therapies targeting pathogenic bacteria (102)(103)(104)(105).…”

Section: Figmentioning

confidence: 99%

Novel Reporter for Identification of Interference with Acyl Homoserine Lactone and Autoinducer-2 Quorum Sensing

Weiland‐Bräuer

Pinnow

Schmitz

2015

Appl Environ Microbiol

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Two reporter strains were established to identify novel biomolecules interfering with bacterial communication (quorum sensing [QS]). The basic design of these Escherichia coli-based systems comprises a gene encoding a lethal protein fused to promoters induced in the presence of QS signal molecules. Consequently, these E. coli strains are unable to grow in the presence of the respective QS signal molecules unless a nontoxic QS-interfering compound is present. The first reporter strain designed to detect autoinducer-2 (AI-2)-interfering activities (AI2-QQ.1) contained the E. coli ccdB lethal gene under the control of the E. coli lsrA promoter. The second reporter strain (AI1-QQ.1) contained the Vibrio fischeri luxI promoter fused to the ccdB gene to detect interference with acyl-homoserine lactones. Bacteria isolated from the surfaces of several marine eukarya were screened for quorum-quenching (QQ) activities using the established reporter systems AI1-QQ.1 and AI2-QQ.1. Out of 34 isolates, two interfered with acylated homoserine lactone (AHL) signaling, five interfered with AI-2 QS signaling, and 10 were demonstrated to interfere with both signal molecules. Open reading frames (ORFs) conferring QQ activity were identified for three selected isolates (Photobacterium sp., Pseudoalteromonas sp., and Vibrio parahaemolyticus). Evaluation of the respective heterologously expressed and purified QQ proteins confirmed their ability to interfere with the AHL and AI-2 signaling processes. Q uorum sensing (QS) is the cell-cell communication betweenbacteria that allows the perception of population density by small signaling molecules-so-called autoinducers-and modifies gene expression in response to the population density. It controls a wide spectrum of processes and phenotypic behaviors, including stress resistance, production of toxins and secondary metabolites, pathogenicity, swarming, and biofilm formation (for a review, see references 1 and 2). In addition to playing important roles in intraspecies and interspecies communication, QS is also involved in host-microbe interactions (3-5). Intraspecific communication of Gram-negative bacteria is based on the production and perception of acylated homoserine lactones (AHLs) synthesized by LuxI homologs. Increasing intracellular concentrations of diffusible AHLs with higher cell densities are perceived by binding of the AHLs to the cognate receptor (e.g., LuxR in Vibrio fischeri), resulting in activation or inhibition of target gene transcription (6, 7). Interspecific communication between different species within one habitat has been demonstrated to depend on the synthesis and detection of the universal autoinducer-2 (AI-2), which can be inhibited by furanones (8, 9). The perception of these signal molecules is achieved either by a two-component regulatory system (e.g., in Vibrio harveyi, where the induced phosphorylation cascade finally results in the induction of specific target genes) (10), or by transportation of the signal molecule into the cell (e.g., in Escherichia coli,...

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“…These enzymes contain the conserved motif HX HXDH and a zinc binding motif and therefore are classified as metallic ␤-lactamases (23). Recent developments in the field of nanotechnology have helped to some extent to overcome problems with drug resistance and biofilm formation through synthesis of bioactive materials (24). Chemically synthesized nanoparticles (NPs) give rise to toxicity, thereby limiting their biomedical applications (25).…”

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confidence: 99%

In Vitro Cytotoxic Effects of Gold Nanoparticles Coated with Functional Acyl Homoserine Lactone Lactonase Protein from Bacillus licheniformis and Their Antibiofilm Activity against Proteus Species

Gopalakrishnan

Pati

Sonawane

et al. 2015

Antimicrob Agents Chemother

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b N-acylated homoserine lactonases are known to inhibit the signaling molecules of the biofilm-forming pathogens. In this study, gold nanoparticles were coated with N-acylated homoserine lactonase proteins (AiiA AuNPs) purified from Bacillus licheniformis. The AiiA AuNPs were characterized by UV-visible spectra, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The synthesized AiiA AuNPs were found to be spherical in shape and 10 to 30 nm in size. Treatment with AiiA protein-coated AuNPs showed maximum reduction in exopolysaccharide production, metabolic activities, and cell surface hydrophobicity and potent antibiofilm activity against multidrug-resistant Proteus species compared to treatment with AiiA protein alone. AiiA AuNPs exhibited potent antibiofilm activity at 2 to 8 M concentrations without being harmful to the macrophages. We conclude that at a specific dose, AuNPs coated with AiiA can kill bacteria without harming the host cells, thus representing a potential template for the design of novel antibiofilm and antibacterial protein drugs to decrease bacterial colonization and to overcome the problem of drug resistance. In summary, our data suggest that the combined effect of the lactonase and the gold nanoparticles of the AiiA AuNPs has promising antibiofilm activity against biofilmforming and multidrug-resistant Proteus species. Biofilm formation by Proteus species has been reported as a source of catheter-associated urinary tract infection that gives rise to serious complications. Proteus vulgaris, Proteus mirabilis, and Proteus penneri are known to cause urinary tract infections in humans (1). Among the Proteus spp., P. vulgaris is capable of forming crystalline biofilms and generating alkaline urine within 24 h (2). Such biofilm-forming pathogenic bacteria are the major cause of many chronic and recurrent infections, such as periodontitis, endocarditis, chronic otitis, and urinary tract and wound infections (3). Moreover, bacteria that adhere to indwelling medical devices such as intravenous catheters, artificial joints, and cardiac pacemakers or to damaged tissue can cause persistent infections through biofilm formation (2, 4, 5). Mature biofilms of Proteus spp. develop on the surface of uroepithelial cells or catheters; organisms within the mushroom-shaped structure regulate a variety of cellular functions, such as glutamine synthesis, autoinducer 2 (AI-2), cyclic dipeptides, and putrescine synthesis (6-8). Catheter-associated urinary tract infections (CAUTIs) affect more than 400,000 patients per year in the United States (9). Antibiotics used to treat these biofilm-forming pathogens are not active against the recalcitrant biofilms; rather, they target their planktonic counterparts, which create selective pressure on the bacteria, which develop resistance to a particular drug (10). Disrupting the multicellular structure of a Proteus biofilm was proposed as one of the most promising strategies for increasing the sensitivity of path...

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Anti-Fouling Chemistry of Chiral Monolayers: Enhancing Biofilm Resistance on Racemic Surface

Cited by 42 publications

References 72 publications

Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications

Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications

Novel Reporter for Identification of Interference with Acyl Homoserine Lactone and Autoinducer-2 Quorum Sensing

In Vitro Cytotoxic Effects of Gold Nanoparticles Coated with Functional Acyl Homoserine Lactone Lactonase Protein from Bacillus licheniformis and Their Antibiofilm Activity against Proteus Species

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