Anti-Gastric Cancer Activity of the Cell-free Culture Supernatant of Serofluid Dish and Lactiplantibacillus plantarum YT013

Zhang, Rentao; Zhou, Zhaoyang; Ma, Yunhao; Du, Kangjia; Sun, Mengze; Zhang, Hao; Tu, Hongyuan; Jiang, Xinrong; Lü, Juan; Tu, Lixue; Niu, Yuqing; Chen, Peng

doi:10.3389/fbioe.2022.898240

Cited by 12 publications

(5 citation statements)

References 46 publications

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“…In another study, L. buchneri FD2 showed selectivity in killing cancer cells compared to the WRL68 normal liver cell line. In addition, research has shown that Lactiplantibacillus plantarum YT013 and the cell-free culture supernatant from serofluid dish could inhibit the proliferation of the cancer cell lines without causing harm to healthy cells 62 .…”

Section: Discussionmentioning

confidence: 99%

The inhibition effects of Lentilactobacillus buchneri-derived membrane vesicles on AGS and HT-29 cancer cells by inducing cell apoptosis

Abedi,

Tafvizi,

Jafari

et al. 2024

Sci Rep

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In recent years, probiotics and their derivatives have been recognized as important therapeutic agents in the fight against cancer. Therefore, this study aimed to investigate the anticancer effects of membrane vesicles (MVs) from Lentilactobacillus buchneri strain HBUM07105 probiotic isolated from conventional and unprocessed yogurt in Arak province, Iran, against gastric and colon cancer cell lines. The MVs were prepared from the cell-free supernatant (CFS) of L. buchneri and characterized using field-emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) and SPS-PAGE techniques. The anticancer activity of MVs was evaluated using MTT, flow cytometry, qRT-PCR techniques, and a scratch assay. The study investigated the anti-adenocarcinoma effect of MVs isolated from L. buchneri on a human gastric adenocarcinoma cell line (AGS) and a human colorectal adenocarcinoma cell line (HT-29) at 24, 48, and 72-h time intervals. The results demonstrated that all prepared concentrations (12.5, 25, 50, 100, and 200 µg/mL) of MVs reduced the viability of both types of human adenocarcinoma cells after 24, 48, and 72 h of treatment. The analysis of the apoptosis results revealed that the percentage of AGS and HT-29 cancer cells in the early and late stages of apoptosis was significantly higher after 24, 48, and 72 h of treatment compared to the untreated cancer cells. After treating both AGS and HT-29 cells with the MVs, the cells were arrested in the G0/G1 phase. These microvesicles demonstrate apoptotic activity by increasing the expression of pro-apoptotic genes (BAX, CASP3, and CASP9). According to the scratch test, MVs can significantly decrease the migration of HT-29 and AGS cancer cells after 24, 48, and 72 h of incubation compared to the control groups. The MVs of L. buchneri can also be considered a potential option for inhibiting cancer cell activities.

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Section: Discussionmentioning

confidence: 99%

The inhibition effects of Lentilactobacillus buchneri-derived membrane vesicles on AGS and HT-29 cancer cells by inducing cell apoptosis

Abedi,

Tafvizi,

Jafari

et al. 2024

Sci Rep

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“…For instance, Enterococcus faecalis has been shown to induce the production of intracellular reactive oxygen species through an oxidative phosphorylation‐independent pathway, resulting in restraint of DNA damage repair and downregulation of cell cycle regulation genes 26 . Another example is Lactiplantibacillus plantarum YT013 , which was reported to inhibit GC cell proliferation and promote cell apoptosis through endogenous mitochondrial apoptosis pathways 27 . In this study, we focus on reporting the role of P. intermedia derived from GC tissues, which appears to promote cell proliferation, migration, and invasion in GC.…”

Section: Discussionmentioning

confidence: 99%

“… 26 Another example is Lactiplantibacillus plantarum YT013 , which was reported to inhibit GC cell proliferation and promote cell apoptosis through endogenous mitochondrial apoptosis pathways. 27 In this study, we focus on reporting the role of P. intermedia derived from GC tissues, which appears to promote cell proliferation, migration, and invasion in GC. These findings contribute to the growing understanding of the potential effect of microbial species on cancer progression.…”

Section: Discussionmentioning

confidence: 99%

Tumor‐derived Prevotella intermedia aggravates gastric cancer by enhancing Perilipin 3 expression

Liang,

Zhou,

Gao

et al. 2024

Cancer Science

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The indigenous microbial milieu within tumorous tissues exerts a pivotal influence on the genesis and advancement of gastric cancer (GC). This investigation scrutinizes the functions and molecular mechanisms attributed to Prevotella intermedia in the malignant evolution of GC. Isolation of P. intermedia from paired GC tissues was undertaken. Quantification of P. intermedia abundance in 102 tissues was accomplished using quantitative real‐time PCR (qRT‐PCR). Assessment of the biological effects of P. intermedia on GC cells was observed using culture medium supernatant. Furthermore, the protein profile of GC cells treated with tumor‐derived P. intermedia was examined through label‐free protein analysis. The functionality of perilipin 3 (PLIN3) was subsequently confirmed using shRNA. Our investigation revealed that the relative abundance of P. intermedia in tumor tissues significantly surpassed that of corresponding healthy tissues. The abundance of P. intermedia exhibited correlations with tumor differentiation (p = 0.006), perineural invasion (p = 0.004), omentum majus invasion (p = 0.040), and the survival duration of GC patients (p = 0.042). The supernatant derived from tumor‐associated P. intermedia bolstered the proliferation, clone formation, migration, and invasion of GC cells. After indirect co‐cultivation with tumor‐derived P. intermedia, dysregulation of 34 proteins, including PLIN3, was discerned in GC cells. Knockdown of PLIN3 mitigated the malignancy instigated by P. intermedia in GC cells. Our findings posit that P. intermedia from the tumor microenvironment plays a substantial role in the malignant progression of GC via the modulation of PLIN3 expression. Moreover, the relative abundance of P. intermedia might serve as a potential biomarker for the diagnosis and prognosis of GC.

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“…It is well known that 5-FU is the most used chemotherapeutic agent in colorectal cancer treatment. It has been approved that Uro-A is able to sensitize colon cancer cells to 5-FU (Zhang et al, 2022).…”

Section: Chemo-sensitizing Agents Derived From Micro-organism Sourcesmentioning

confidence: 99%

Therapeutic, chemo-sensitizing, protective effects of natural products against cancerous cells: Preclinical and clinical studies

El-Said,

El-Feki

2024

Biological and Biomedical Journal

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There are times when it is challenging to stop chemotherapy side effects from happening when the patient is receiving treatment. Nowadays, the treatment with chemotherapy combinations is frequently utilized to treat different forms of cancer. The key benefit of utilizing numerous medications was thought to be the ability to lower dosages, which may result in decrease resistance and the maintenance of the same efficacy or, with a higher efficacy, or a synergistic impact. It has been suggested that pharmacological combinations consisting of natural products (NPs) and dietary supplements can have benefits comparable to those of traditional chemotherapy treatments, but with fewer side effects. Pre-clinical and clinical studies were carried out to evaluate how NPs worked against malignant cells. Thus, the optimal balance between potential side effects and anticancer activity is desperately needed. The therapeutic, chemosensitizing, and protective activities of NPs against malignant cells are covered in this review.

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Anti-Gastric Cancer Activity of the Cell-free Culture Supernatant of Serofluid Dish and Lactiplantibacillus plantarum YT013

Abstract: Graphical AbstractThe overall mechanisms for the induction of apoptosis in AGS cells by the CCS2 prepared from the selected L. plantarum YT013 were summarized as the picture.

Cited by 12 publications

References 46 publications

The inhibition effects of Lentilactobacillus buchneri-derived membrane vesicles on AGS and HT-29 cancer cells by inducing cell apoptosis

The inhibition effects of Lentilactobacillus buchneri-derived membrane vesicles on AGS and HT-29 cancer cells by inducing cell apoptosis

Tumor‐derived Prevotella intermedia aggravates gastric cancer by enhancing Perilipin 3 expression

Therapeutic, chemo-sensitizing, protective effects of natural products against cancerous cells: Preclinical and clinical studies

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