Anti-Hepatoma Compound Determination by the Method of Spectrum Effect Relationship, Component Knock-Out, and UPLC-MS2 in Scheflera heptaphylla (L.)Frodin Harms and Its Mechanism

Liu, Xuqiang; Jiang, Nan; Xu, Xiaoqing; Liu, Cunyu; Liu, Zhenhua; Zhang, Yan

doi:10.3389/fphar.2020.01342

Cited by 13 publications

(9 citation statements)

References 32 publications

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“…Afterward, the PVDF membranes were then incubated with diluted secondary antibodies for 1–2 h at room temperature. β-Actin was used as an internal reference protein, and PVDF membranes were developed and fixed to observe target protein expression changes ( 22 , 23 ).…”

Section: Methodsmentioning

confidence: 99%

“…Later, the protein transfer to the PVDF membrane was accomplished using an ice bath, the proteins were blocked by BSA for 1 h at room temperature, and the primary antibodies (Bcl-2, Bax, caspase-3) were added proportionally and incubated overnight at 4 • C. Afterward, the PVDF membranes were then incubated with diluted secondary antibodies for 1-2 h at room temperature. β-Actin was used as an internal reference protein, and PVDF membranes were developed and fixed to observe target protein expression changes (22,23).…”

Section: Western Blotmentioning

confidence: 99%

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Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells

Feng

Zhou²,

Zhou³

et al. 2023

Front. Nutr.

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To study the anti-tumor effect of Cistanche deserticola Y. Ma, HepG2 cells were treated with 0, 3.5, 10.5, 21, 31.5, and 42 μg/ml of total glycosides (TG) from Cistanche deserticola. The HepG2 cell survival rate and 50% inhibition concentration (IC50) were detected using the CCK-8 method, and the level of reactive oxygen species (ROS) was detected by using a DCFH-DA fluorescence probe. Finally, a Seahorse XFe24 energy analyzer (Agilent, United States) was used to detect cell mitochondrial pressure and glycolytic pressure. The results showed that TG could reduce the survival rate of HepG2 cells and that the IC50 level was 35.28 μg/ml. With increasing TG concentration, the level of ROS showed a concentration-dependent upward trend. Energy metabolism showed that each dose group of TG could significantly decline the mitochondrial respiratory and glycolytic functions of HepG2 cells. In conclusion, TG could significantly inhibit the mitochondrial respiration and glycolysis functions of HepG2 cells, increase the level of ROS, and inhibit cell proliferation. Thus, this experiment pointed out that Cistanche deserticola can be used as a source of anti-cancer foods or drugs in the future. However, further studies on its mechanisms and clinical applications are needed.

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Section: Methodsmentioning

confidence: 99%

Section: Western Blotmentioning

confidence: 99%

Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells

Feng

Zhou²,

Zhou³

et al. 2023

Front. Nutr.

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“…By comparison, chemotherapeutic drugs are targeted and quickly, but they could produce many side effects, such as myelosuppression, decreased immune function, organ damage, hair loss and so on. 149 …”

Section: Ethnopharmacologymentioning

confidence: 99%

A review: the botany, ethnopharmacology, phytochemistry, pharmacology of Cinnamomi cortex

et al. 2021

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“…This method has been successfully applied to reveal the material basis of traditional Chinese medicines. By correlating high-performance liquid chromatography (HPLC) chromatograms with cell inhibitory activities, Liu et al [ 17 ] found that three compounds in Scheflera heptaphylla were positively correlated with the anti-hepatoma effect. Zhou et al [ 18 ] found that ginsenoside Ro is responsible for the antiproliferative activity of Panax ginseng .…”

Section: Introductionmentioning

confidence: 99%

Spectrum-Effect Relationship-Based Strategy Combined with Molecular Docking to Explore Bioactive Flavonoids from Sceptridium ternatum

et al. 2022

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Sceptridium ternatum is a herbaceous plant with significant potential for pharmaceutical and cosmetic applications. In this study, we established a spectrum-effect relationship-based strategy to investigate the bioactive basis and tissue distribution in S. ternatum. First, a phytochemical analysis on the ethanol extracts from roots, stems, and leaves of S. ternatum was performed using the colorimetric method, high-performance liquid chromatography–ultraviolet (HPLC–UV), and high-performance liquid chromatography–electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC–ESI-Q-TOF-MS/MS). Then, radical scavenging assays and the lipopolysaccharide-stimulated RAW 264.7 cell model were used to estimate the antioxidant and anti-inflammatory activities, respectively. Spectrum-effect relationship analysis and molecular docking were further employed to evaluate the correlation between the phytochemical profile and anti-inflammatory activity. Our results demonstrate that S. ternatum leaves contained the most abundant flavonoids and exerted the best biological activities. Their IC50 values for scavenging 2,2ʹ-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) and 1,1-diphenyl-2-picrylhydrazyl radicals were 2.43 ± 0.13 and 5.36 ± 0.54 mg/mL, respectively. In lipopolysaccharide-stimulated RAW 264.7 cells, the leaf extract caused the greatest reduction in nitric oxide production (38.15%) and interleukin-6 release (110.86%). Spectrum-effect relationship analysis and molecular docking indicated that quercetin 3-O-rhamnoside-7-O-glucoside possessed high anti-inflammatory activity by binding with interleukin-6. In conclusion, S. ternatum is a rich source of bioactive flavonoids with potential for exploitation in the prevention and treatment of oxidative stress and inflammation-related pathologies.

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Anti-Hepatoma Compound Determination by the Method of Spectrum Effect Relationship, Component Knock-Out, and UPLC-MS2 in Scheflera heptaphylla (L.)Frodin Harms and Its Mechanism

Abstract: These results demonstrated that Compound A was the main anti-hepatoma active component in the S. heptaphylla leaves. It achieved the effect of promoting apoptosis of Huh7 cells by regulating the levels of ROS and Bcl-2 family protein in mitochondrial apoptosis pathway.

Cited by 13 publications

References 32 publications

Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells

Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells

A review: the botany, ethnopharmacology, phytochemistry, pharmacology of Cinnamomi cortex

Spectrum-Effect Relationship-Based Strategy Combined with Molecular Docking to Explore Bioactive Flavonoids from Sceptridium ternatum

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