Anti-hepatotoxic activities of Hibiscus sabdariffa L. in animal model of streptozotocin diabetes-induced liver damage

Abstract

BackgroundFlavonoid-rich aqueous fraction of methanolic extract of Hibiscus sabdariffa calyx was evaluated for its anti-hepatotoxic activities in streptozotocin-induced diabetic Wistar rats.MethodsDiabetes Mellitus was induced in Wistar rats by a single i.p injection of 80 mg/kg b.w. streptozotocin (STZ) dissolved in 0.1 M citrate buffer (pH 6.3).ResultsThe ameliorative effects of the extract on STZ-diabetes induced liver damage was evident from the histopathological analysis and the biochemical parameters evaluated in the serum and liver homogenates. Reduced levels of glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (3.76 ± 0.38 μM, 0.42 ± 0.04 U/L, 41.08 ± 3.04 U/ml, 0.82 ± 0.04 U/L respectively) in the liver of diabetic rats were restored to a near normal level in the Hibiscus sabdariffa -treated rats (6.87 ± 0.51 μM, 0.72 ± 0.06 U/L, 87.92 ± 5.26 U/ml, 1.37 ± 0.06 U/L respectively). Elevated levels of aspartate amino transferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) in the serum of diabetic rats were also restored in Hibiscus sabdariffa -treated rats. Examination of stained liver sections revealed hepatic fibrosis and excessive glycogen deposition in the diabetic rats. These pathological changes were ameliorated in the extract-treated rats.ConclusionThe anti-hepatotoxic activity of Hibiscus sabdariffa extract in STZ diabetic rats could be partly related to its antioxidant activity and the presence of flavonnoids.

Comparison of Hibiscus sabdariffa L. Extract and Enalapril with Regard to Their Effect on Lung Fibrosis in a Bleomycin-induced Rat Model of Lung Fibrosis

“…Inpress(Inpress):e38798. 5 PCA through its high antiradical activity (19,39). The results showed that there is no significant difference between the group administered 400 mg/kg HSE and the control group with regard to the lung tissue MDA levels.…”

“…For example, Hibiscus sabdariffa L. (Roselle; Malvaceae), which is known to contain several antioxidants such as protocatechuic acid (PCA) and anthocyanins, has potential as an ethnomedical remedy for diabetes, jaundiced liver, hypertension, obesity and urinary dysfunctions. These effects have been confirmed in humans and experimental animals (19). Recent studies have demonstrated that H. sabdariffa L. extract (HSE) exhibits antioxidant, antidiabetic, antibacterial, antihypertensive, antiproliferative, anticholesterol, nephroprotective, cardioprotective, hepatoprotective and renal-diuretic activities (20,21).…”

Cucurbitacin Attenuates Hyperglycemia by Increasing Glut2 Through Pi3k-Pakt in the Hepatocytes of Streptozotocin Induced Diabetic Rats

“…Diabetic rats showing extensive area of fibrosis and collagen production which is the main cause of histological changes in the STZ induced diabetic rats. [33] Oral treatment with cucurbitacin protects the above abnormalities by suppressing the effects of STZ. Upon treatment with cucurbitacin, insulin…”

Natural Products for Prevention and Treatment of Chemical‐Induced Liver Injuries

“…It was observed that a vegetable like cactus ( Opuntia ficus‐indica ) could reestablish hematological parameters, decrease lipid peroxidation, and restore enzyme activities in rat liver injured by methotrexate, a chemotherapy medication used to treat cancer (Akacha, Rebai, Amri, & Zourgui, ). In counteracting the liver injury caused by streptozotocin, antioxidant activity was demonstrated in Hibiscus sabdariffa L. extract, as well as spent mushroom compost polysaccharide and its hydrolysates, leading to the improved parameters and liver function as well as lowered blood lipids (Adeyemi, Ukwenya, Obuotor, & Adewole, ; Liu et al., ). In another rat model, whole grain of Kamut khorasan wheat ameliorated doxorteubicin‐induced oxidative damage in the liver (Akacha et al., ).…”