Anti-HER2 antibody and ScFvEGFR-conjugated antifouling magnetic iron oxide nanoparticles for targeting and magnetic resonance imaging of breast cancer

Chen, Hongwei; Wang, Liya; Yu, Qiqi; Qian, Weiping; Tiwari, Diana; Yi, Hong; Wang, Andrew Y.; Huang, Jing; Yang, Lily; Mao, Hui

doi:10.2147/ijn.s49069

Cited by 32 publications

(20 citation statements)

References 53 publications

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“…8,21–24 Although these materials have shown the capability of reducing biofouling given their inert structures, the facile functionalization of these materials for introducing targeting ligands is challenging. 24–26 Currently, the most commonly used strategy to overcome non-specific protein adsorption and cell uptake is to graft PEG onto the nanoparticle surface, 26–31 because PEG facilitates the formation of a hydrate film around the particle through hydrogen bonding with water, which attenuates the protein interactions, cell uptake, 27 and immunogenic response. 32–40 In order to immobilize targeting ligands onto nanoparticles with PEG based coating, the typical approach is to introduce additional moieties that contain reactive groups (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…-NH 2 or -COOH) for functionalization. 24,29–31 Since surface charge may promote the phagocytosis process, 26,41–44 the surface charge introduced from the reactive groups would “offset” the anti-biofouling effect from PEG. Hence, a coating material that bears reactive functional groups yet still exhibits anti-biofouling property is desirable.…”

Section: Introductionmentioning

confidence: 99%

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PEG-b-AGE polymer coated magnetic nanoparticle probes with facile functionalization and anti-fouling properties for reducing non-specific uptake and improving biomarker targeting

Lin

Wang

et al. 2015

J. Mater. Chem. B

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Non-specific surface adsorption of bio-macromolecules (e.g. proteins) on nanoparticles, known as biofouling, and the uptake of nanoparticles by the mononuclear phagocyte system (MPS) and reticuloendothelial system (RES) lead to substantial reduction in the efficiency of target-directed imaging and delivery in biomedical applications of engineered nanomaterials in vitro and in vivo. In this work, a novel copolymer consisting of blocks of poly ethylene glycol and allyl glycidyl ether (PEG-b-AGE) was developed for coating magnetic iron oxide nanoparticles (IONPs) to reduce non-specific protein adhesion that leads to formation of “protein corona” and uptake by macrophages. The facile surface functionalization was demonstrated by using targeting ligands of a small peptide of RGD or a whole protein of transferrin (Tf). The PEG-b-AGE coated IONPs exhibited anti-biofouling properties with significantly reduced protein corona formation and non-specific uptake by macrophages before and after the surface functionalization, thus improving targeting of RGD-conjugated PEG-b-AGE coated IONPs to integrins in U87MG glioblastoma and MDA-MB-231 breast cancer cells that overexpress αvβ3 integrins, and Tf-conjugated PEG-b-AGE coated IONPs to transferrin receptor (TfR) in D556 and Daoy medulloblastoma cancer cells with high overexpression of transferrin receptor, compared to respective control cell lines. Magnetic resonance imaging (MRI) of cancer cells treated with targeted IONPs with or without anti-biofouling PEG-b-AGE coating polymers demonstrated the target specific MRI contrast change using anti-biofouling PEG-b-AGE coated IONP with minimal off-targeted background compared to the IONPs without anti-biofouling coating, promising the highly efficient active targeting of nanoparticle imaging probes and drug delivery systems and potential applications of imaging quantification of targeted biomarkers.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PEG-b-AGE polymer coated magnetic nanoparticle probes with facile functionalization and anti-fouling properties for reducing non-specific uptake and improving biomarker targeting

Lin

Wang

et al. 2015

J. Mater. Chem. B

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“…We expect that the antifouling property of reducing opsnonization through an effective polymer coating will hinder uptake of administered nanoparticles by immune cells in the reticuloendothelial system (RES) and thus enhance the effective accumulation of the PEO-PPDSM AuNPs at target sites, consistent with our previous studies. 42,43 …”

Section: Resultsmentioning

confidence: 99%

Thiol-reactive amphiphilic block copolymer for coating gold nanoparticles with neutral and functionable surfaces

et al. 2014

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Nanoparticles designed for biomedical applications are often coated with polymers containing reactive functional groups, such as –COOH and –NH2, to conjugate targeting ligands or drugs. However, introducing highly charged surfaces promotes binding of the nanoparticles to biomolecules in biological systems through ionic interactions, causing the nanoparticles to aggregate in biological environments and consequently undergo strong non-specific binding to off-target cells and tissues. Developing a unique polymer with neutral surfaces that can be further functionalized directly would be critical to develop suitable nanomaterials for nanomedicine. Here, we report a thiol-reactive amphiphilic block copolymer poly(ethylene oxide)-block-poly(pyridyldisulfide ethylmeth acrylate) (PEO-b-PPDSM) for coating gold nanoparticles (AuNPs). The resultant polymer-coated AuNPs have almost neutral surfaces with slightly negative zeta potentials from -10 to 0 mV over a wide pH range from 2 to 12. Although the zeta potential is close to zero we show that the PEO-b-PPDSM copolymer-coated AuNPs have both good stability in various physiological conditions and reduced non-specific adsorption of proteins/biomolecules. Because of the multiple pyridyldisulfide groups on the PPDSM block, these individually dispersed nanocomplexes with an overall hydrodynamic size around 43.8 nm can be directly functionalized via disulfide-thiol exchange chemistry.

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“…Most recent developments relate to cell labeling using positron emitters because, in human, PET imaging offers better resolution and more precise quantification compared to SPECT. Copper-64 is an interesting candidate, with good imaging properties and a relatively long half-life of 12.7 h. 64 Cu-pyruvaldehydebis(N4-methylthiosemicarbazone ( 64 Cu-PTSM) was thus used to label C6 glioma cells, as the lipophilic complex is readily taken up in cells. A good cell labeling yield, but a significant efflux rate from cells was observed (12).…”

Section: Radioactive (Spect Pet)mentioning

confidence: 99%

“…Alternatively, cells may be transfected to express cell-surface receptors for peptides as, for instance, the human glucagon-like peptide 1 receptor gene and imaged with the peptide labeled with fluorine-18 (35). A similar approach was used to detect transplanted pancreatic islet cells that express glucagon-like peptide 1 receptor (GLP-1R) by PET imaging after the injection of 64 Cu-DO3A-VS-Cys40-Exendin-4, showing persistent and specific uptake in the mouse pancreas (36). The mutated version of the dopamine receptor, D2R80A, that internalize 18 F-Fallypride, has also been proposed for imaging mesenchymal stem cells (37,38).…”

Section: Radioactive Imagingmentioning

confidence: 99%

Cell Tracking in Cancer Immunotherapy

et al. 2020

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The impressive development of cancer immunotherapy in the last few years originates from a more precise understanding of control mechanisms in the immune system leading to the discovery of new targets and new therapeutic tools. Since different stages of disease progression elicit different local and systemic inflammatory responses, the ability to longitudinally interrogate the migration and expansion of immune cells throughout the whole body will greatly facilitate disease characterization and guide selection of appropriate treatment regiments. While using radiolabeled white blood cells to detect inflammatory lesions has been a classical nuclear medicine technique for years, new non-invasive methods for monitoring the distribution and migration of biologically active cells in living organisms have emerged. They are designed to improve detection sensitivity and allow for a better preservation of cell activity and integrity. These methods include the monitoring of therapeutic cells but also of all cells related to a specific disease or therapeutic approach. Labeling of therapeutic cells for imaging may be performed in vitro, with some limitations on sensitivity and duration of observation. Alternatively, in vivo cell tracking may be performed by genetically engineering cells or mice so that may be revealed through imaging. In addition, SPECT or PET imaging based on monoclonal antibodies has been used to detect tumors in the human body for years. They may be used to detect and quantify the presence of specific cells within cancer lesions. These methods have been the object of several recent reviews that have concentrated on technical aspects, stressing the differences between direct and indirect labeling. They are briefly described here by distinguishing ex vivo (labeling cells with paramagnetic, radioactive, or fluorescent tracers) and in vivo (in vivo capture of injected radioactive, fluorescent or luminescent tracers, or by using labeled antibodies, ligands, or pre-targeted clickable substrates) imaging methods. This review focuses on cell tracking in specific therapeutic applications, namely cell therapy, and particularly CAR (Chimeric Antigen Receptor) T-cell therapy, which is a fast-growing research field with various therapeutic indications. The potential impact of imaging on the progress of these new therapeutic modalities is discussed.

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Anti-HER2 antibody and ScFvEGFR-conjugated antifouling magnetic iron oxide nanoparticles for targeting and magnetic resonance imaging of breast cancer

Cited by 32 publications

References 53 publications

PEG-b-AGE polymer coated magnetic nanoparticle probes with facile functionalization and anti-fouling properties for reducing non-specific uptake and improving biomarker targeting

PEG-b-AGE polymer coated magnetic nanoparticle probes with facile functionalization and anti-fouling properties for reducing non-specific uptake and improving biomarker targeting

Thiol-reactive amphiphilic block copolymer for coating gold nanoparticles with neutral and functionable surfaces

Cell Tracking in Cancer Immunotherapy

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