Anti-herpesviral property and mode of action of a polysaccharide from brown seaweed (Hydroclathrus clathratus)

Wang, Hui; Ooi, Vincent E.C.; Ang, Put O.

doi:10.1007/s11274-010-0348-0

Cited by 12 publications

(4 citation statements)

References 34 publications

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“…Finally, there are rare mutants with normal tkv activity but with impaired viral DNApol activity to ACV-triphosphate. This is caused by multiple mutations in the gene encoding tkv and DNApol [38][39][40][41][42][43]. As an alternative to this problem, there are agents that act through a mechanism of action different from ACV.…”

Section: Herpes Simplex Virusmentioning

confidence: 99%

Antiherpetic Plants: A Review of Active Extracts, Isolated Compounds, and Bioassays

Silva-Mares

Torres‐López

Rivas-Galindo

2016

Natural Product Communications

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Herpes simplex is a disease that is widely distributed throughout the world. It is caused by herpes simplex virus type 1 (HSV-1) and simplex virus type 2 (HSV-2). The drugs of choice for treatment are acyclovir (ACV), Penciclovir (PCV) and other guanine analogues, which have the same mechanism of action. However, due to the constant increase of ACV-resistant strains in immunocompromised patients, it is necessary to find new treatment alternatives. It has been shown that natural products are a good alternative for the treatment of these diseases as well as being an excellent source of compounds with anti-herpetic activity, which may be useful for the development of new drugs and act through a mechanism of action different from ACV and PCV. This paper compiles reports on extracts and compounds isolated from plants that have anti-herpetic activity. We present an analysis of the solvents most widely used for extraction from plants as well as cells and commonly used methods for evaluating cytotoxic and anti-herpetic activity. Families that have a higher number of plants with anti-herpetic activity are evaluated, and we also highlight the importance of studies of mechanisms of action of extracts and compounds with anti-herpetic activity.

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Section: Herpes Simplex Virusmentioning

confidence: 99%

Antiherpetic Plants: A Review of Active Extracts, Isolated Compounds, and Bioassays

Silva-Mares

Torres‐López

Rivas-Galindo

2016

Natural Product Communications

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“…The chemical constituents isolated Methanol extract of Hydroclathrus clathratus suppresses matrix metalloproteinase-9 in T24 bladder carcinoma cells by suppressing the NF-κB and MAPK pathways from this seaweed are commonly polysaccharides H3-a1 and H3-b1, which have anti-viral and anti-cancer properties (12). Some components of H. clathratus affect the growth of human acute promyelocytic leukemia, human breast carcinoma and human hepatocellular carcinoma cancer cell lines (13).…”

Section: Introductionmentioning

confidence: 99%

Methanol extract of Hydroclathrus clathratus suppresses matrix metalloproteinase-9 in T24 bladder carcinoma cells by suppressing the NF-κB and MAPK pathways

Jayasooriya

Choi²,

Moon³

et al. 2011

Oncol Rep

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Abstract.Hydroclathrus clathratus is a brown marine seaweed and its extract possessing anti-cancer, anti-herpetic and anticoagulant activities is a traditional drug and health food in Korea, Japan and China. However, little is known about the mechanism by which the methanol extract of H. clathratus (MEHC) inhibits invasion of cancer cells. In the present study, we investigated the effects of MEHC on the expression of matrix metalloproteinase-9 (MMP-9) in T24 human bladder carcinoma cells. Our findings showed that MMP-9 activity was significantly increased in response to tumor necrosis factor-α (TNF-α). However, treatment with MEHC substantially reversed TNF-α-induced MMP-9 activity. A matrigel invasion assay also showed that MEHC reduced TNF-α-induced invasion of T24 bladder carcinoma cells. We also found that MEHC significantly downregulated the expression of the MMP-9 gene induced by TNF-α stimulation. Furthermore, we investigated the effects of MEHC on nuclear factor (NF)-κB activity, which is a potential transcriptional factor for regulating many invasive genes including MMP-9. MEHC suppressed NF-κB activity by suppressing IκB degradation and nuclear translocation of the NF-κB p65 and p50 subunits. TNF-α-induced phosphorylation of phosphatidyl-inositol 3 kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK) was significantly downregulated in the presence of MEHC. Taken together, these results indicate that MEHC is a potential anti-invasive agent by suppressing TNF-α-induced cancer cell invasion and by specifically inhibiting NF-κB and MAPKs, as well as downstream target genes such as MMP-9.

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“…Chemical constituents commonly isolated from this seaweed are the polysaccharides H3-a1 and H3-b1, which have anti-viral and anti-cancer properties [20]. Some components of H. clathratus specifically affect the growth of human acute promyelocytic leukemia cells (HL-60), human breast carcinoma cells (MCF-7), and human hepatocellular carcinoma cancer cells [21].…”

Section: Introductionmentioning

confidence: 99%

Methanol Extract of <i>Hydroclathrus clathratus</i> Inhibits Production of Nitric Oxide, Prostaglandin E<sub>2</sub> and Tumor Necrosis Factor-&#945 in Lipopolysaccharidestimulated BV2 Microglial Cells via Inhibition of NF-&#954B Activity

Jayasooriya¹,

Moon²,

Chol³

et al. 2011

Trop. J. Pharm Res

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Purpose: Hydroclathrus clathratus is a brown marine seaweed known to possess anti-cancer, anti-herpetic, and anti-coagulant activities. The present study is aimed at investigating some anti-inflammatory effects of H. clathratus. Methods: We investigated the anti-inflammatory effects of the methanol extract of H. clathratus (MEHC) by expression of mRNA and protein using RT-PCR and Western blot analysis in lipopolysaccharide (LPS)-stimulated BV2 microglial cells. The level of nitric oxide (NO) production was analyzed using Griess reaction.The release of prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) were determined using sandwich ELISA. NF-κB activation was detected using EMSA methods. Results: The results obtained indicate that the extract (MEHC) inhibited LPS-induced NO, PGE2, and TNF-α production without any significant cytotoxicity (p < 0.05). MEHC also inhibited production of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and TNF-α mRNA in LPS-stimulated BV2 microglial cells. In addition, MEHC significantly reduced (p < 0.05) nuclear translocation of the nuclear factor-κB (NF-κB) subunits, p50 and p65, and its DNA-binding activity in LPS-stimulated BV2 microglial cells. Conclusion: These results suggest that MEHC suppresses the induction of TNF-α, as well as iNOS and COX-2 expression, by blocking LPS-induced NF-κB activation.

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Anti-herpesviral property and mode of action of a polysaccharide from brown seaweed (Hydroclathrus clathratus)

Cited by 12 publications

References 34 publications

Antiherpetic Plants: A Review of Active Extracts, Isolated Compounds, and Bioassays

Antiherpetic Plants: A Review of Active Extracts, Isolated Compounds, and Bioassays

Methanol extract of Hydroclathrus clathratus suppresses matrix metalloproteinase-9 in T24 bladder carcinoma cells by suppressing the NF-κB and MAPK pathways

Methanol Extract of <i>Hydroclathrus clathratus</i> Inhibits Production of Nitric Oxide, Prostaglandin E<sub>2</sub> and Tumor Necrosis Factor-&#945 in Lipopolysaccharidestimulated BV2 Microglial Cells via Inhibition of NF-&#954B Activity

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