Anti-Herpetic Activities of Chemical Components from the Brazilian Red Alga <i>Plocamium Brasiliense</i>

Ferreira, Wilton José; Amaro, Rodrigo Ledesma; Cavalcanti, Diana Negrão; Rezende, Cláudia M.; Silva, Viveca Antonia Giongo Galvão da; Barbosa, Juliana Eymara; Paixão, Izabel Christina Nunes de Palmer; Teixeira, Valéria Laneuville

doi:10.1177/1934578x1000500802

Cited by 6 publications

(5 citation statements)

References 23 publications

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“…Although studies of the chemical ecology of P. brasiliense are scarce, it was demonstrated that P. brasiliense monoterpenes have high anti-herbivore activity Vasconcelos 2014). In an applied context, these molecules showed activity as herbicides (Fonseca et al 2012) and against both herpes virus (Ferreira et al 2010, Pinto et al 2014) and snake venom (Claudino et al 2014). Thus, the aims of the present work were: (i) to describe the ultrastructure of P. brasiliense CC using optical (OM), transmission (TEM), and scanning electron microscopy (SEM); (ii) to localize mevalonic acid enzyme activity inside CC by using a specific cytochemical assay; and (iii) to investigate the AF activity of P. brasiliense halogenated monoterpenes using in vitro bioassays.…”

mentioning

confidence: 99%

Mevalonosomes: specific vacuoles containing the mevalonate pathway in Plocamium brasiliense cortical cells (Rhodophyta)

Paradas

Crespo

Salgado

et al. 2015

Journal of Phycology

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This paper has identified, for the first time in a member of the Rhodophyta, a vacuolar organelle containing enzymes that are involved in the mevalonate pathway-an important step in red algal isoprenoid biosynthesis. These organelles were named mevalonosomes (Mev) and were found in the cortical cells (CC) of Plocamium brasiliense, a marine macroalgae that synthesizes several halogenated monoterpenes. P. brasiliense specimens were submitted to a cytochemical analysis of the activity of the 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS). Using transmission electron microscopy (TEM), we confirmed the presence of HMGS activity within the Mev. Because HMGS is necessary for the biosynthesis of halogenated monoterpenes, we isolated a hexanic fraction (HF) rich in halogenated monoterpenes from P. brasiliense that contained a pentachlorinated monoterpene as a major metabolite. Because terpenes are often related to chemical defense, the antifouling (AF) activity of pentachlorinated monoterpene was tested. We found that the settlement of the mussel Perna perna was reduced by HF treatment (2.25 times less than control; 40% and 90% of fouled surface, respectively; P = 0.001; F9,9 = 1.13). The HF (at 10 μg · mL(-1) ) also inhibited three species of fouling microalgae (Chlorarachnion reptans, Cylindrotheca cloisterium, and Exanthemachrysis gayraliae), while at a higher concentration (50 μg · mL(-1) ), it inhibited the bacteria Halomonas marina, Polaribacter irgensii, Pseudoalteromonas elyakovii, Shewanella putrefaciens, and Vibrio aestuarianus. The AF activity of P. brasiliense halogenated monoterpenes and the localization of HMGS activity inside Mev suggest that this cellular structure found in CC may play a role in thallus protection against biofouling.

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mentioning

confidence: 99%

Mevalonosomes: specific vacuoles containing the mevalonate pathway in Plocamium brasiliense cortical cells (Rhodophyta)

Paradas

Crespo

Salgado

et al. 2015

Journal of Phycology

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“…An initial study was conducted by Claudino et al [ 34 ] analyzing the inhibitory action of P. brasiliense extract against the toxic effects of L. muta snake venom. Apart from antivenom activities, some biological and pharmacological properties have been found in these algae and their derived products, such as defense against marine herbivores and antiviral activity [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…These marine algae produce acyclic and cyclic halogenated monoterpenes, of which more than 100 molecules have been isolated [ 29 , 30 ]. Such molecules have shown biological activities including antimicrobial, antifungal, ichthyotoxic, cytotoxic and insecticidal properties as well as antiviral activity against herpes virus [ 31 , 32 ]. Moreover, P. brasiliense may be used as herbicide or even as food [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, P. brasiliense may be used as herbicide or even as food [ 33 ]. It has been shown that three products isolated from P. brasiliense inhibited some toxic in vitro and in vivo activities of B. jararaca venom: monoterpene 1 (8-bromo-3,4,7-trichloro-3,7-dimethyl-1E, 5E-octadiene), monoterpene 2 (1,8-dibromo-3,4,7-trichloro-3,7-dimethyl-1E, 5E-octadiene) and a cholesterol [ 30 , 32 , 34 ]. Their chemical structures are shown in Figure 1 .…”

Section: Introductionmentioning

confidence: 99%

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The red seaweed Plocamium brasiliense shows anti-snake venom toxic effects

Silva¹,

Domingos²,

Fonseca³

et al. 2015

J Venom Anim Toxins incl Trop Dis

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BackgroundSnakebite is considered a neglected tropical disease by the World Health Organization. In Brazil, about 70% of the envenomation cases are caused by Bothrops snakes. Its venom may provoke hemorrhage, pain, necrosis, hemolysis, renal or cardiac failure and even death in victims. Since commercial antivenom does not efficiently neutralize the local toxic effects of venoms, natural products have been tested in order to provide alternative or complementary treatment to serum therapy. Therefore, the present study aimed to evaluate the ability of the seaweed Plocamium brasiliense and its active derivatives to neutralize hemorrhagic, edematogenic, hemolytic, coagulant and proteolytic activities of B. jararaca venom.MethodsSpecimens of P. brasiliense were collected in Rio de Janeiro state, Brazil, dried and submitted to oil extraction using four solvents of increasing polarities, n-hexane (HEX), dichloromethane (DCM), ethyl acetate (ETA) and hydroalcoholic solution (HYD). The solvents were evaporated, yielding HEX, DCM, ETA and HYD extracts. Further, all extracts were dissolved in dimethylsulfoxide. In addition, two monoterpenes (8-bromo-3,4,7-trichloro-3,7-dimethyl-1E, 5E-octadiene and 1,8-dibromo-3,4,7-trichloro-3,7-dimethyl-1E, 5E-octadiene) and a cholesterol fraction were isolated from the extract of P. brasiliense prepared in hexane. Algal samples were incubated for 30 minutes with B. jararaca venom, and then tested for lethality; hemorrhagic, edematogenic, hemolytic, coagulant and proteolytic effects.ResultsMost of the algal extracts inhibited the toxic effects with different potencies. The DCM extract was the most effective, since it inhibited all types of toxic activity. On the other hand, the HYD extract failed to inhibit any effect. Moreover, the isolated products inhibited proteolysis and protected mice from hemorrhage in 30% of the cases, whereas 8-bromo-3,4,7-trichloro-3,7-dimethyl-1E, 5E-octadiene inhibited 100% and 20% of the hemorrhagic and proteolytic activities, respectively. None of the algal products were toxic to mice.ConclusionSeaweeds may be a promising source of inhibitors against toxic effects caused by B. jararaca envenomation, which may contribute to antivenom treatment.

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“…It was observed that the dichloromethane extract produced the strongest inhibition of seed germination by 35.0% and 14.0%, radicle elongation by 52.0% and 41.7%, and hypocotyl development by 17.1% and 25.5%, respectively, in M. pudica and S. obtusifolia (Fonseca et al 2012). Previous work carried out by the same research group on the chemotaxonomic analysis of the Brazilian red seaweed and, more specifically, the fractionation of the dichloromethane seaweed extract, revealed richness in halogenated monoterpenes (Ferreira et al 2010; Vasconcelos et al 2010). Fonseca et al (2012) suggested that the halogenated monoterpenes were likely responsible for or played a key role in the phytotoxic activities displayed by the extract.…”

Section: Bioherbicidesmentioning

confidence: 99%

The potential of seaweeds as a rich natural source for novel bioherbicide formulation/development

Chukwuma,

Tan,

Hughes

et al. 2024

Weed Sci

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Recently, there has been emphasis on the need to shift away from the use of synthetic chemical herbicides to low risk alternatives derived from natural sources. This is aimed at lowering or averting the negative impact synthetic herbicides have on the environment and to deal with the emergence of weed species becoming resistant to these chemicals. As a result, more stringent measures or an outright ban on the use of most synthetic herbicides have been put in place by regulatory bodies. As seaweeds are abundant resources in the marine environment that have the capacity to produce diverse bioactive compounds, they could serve as sustainably viable natural, low-risk alternatives/sources to explore for potential phytotoxic capabilities. This could in turn help to enhance or boost the availability of effective solutions in the global bioherbicide market. This review highlights the prospects of using seaweeds as novel biopesticides for the control and management of various plant pests including weed species, and for the development of sustainable agriculture/forestry practices. More specifically, the review focuses on their use as a rich natural source for novel bioherbicide development, a potential that has remained underexplored for many years. However, to unlock the full potential of seaweed-derived bioherbicides and to create a potential path towards its development, increased research and development efforts are urgently needed. This is to tackle and overcome possible constraints posed in this novel area such as variability in seaweed chemical composition, formulation technologies, stability and efficacy of seaweed bioactive compounds, cost and scalability, environmental considerations, etc.

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Anti-Herpetic Activities of Chemical Components from the Brazilian Red Alga Plocamium Brasiliense

Cited by 6 publications

References 23 publications

Mevalonosomes: specific vacuoles containing the mevalonate pathway in Plocamium brasiliense cortical cells (Rhodophyta)

Mevalonosomes: specific vacuoles containing the mevalonate pathway in Plocamium brasiliense cortical cells (Rhodophyta)

The red seaweed Plocamium brasiliense shows anti-snake venom toxic effects

The potential of seaweeds as a rich natural source for novel bioherbicide formulation/development

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