Anti-HSV-1 effect of dihydromyricetin from Ampelopsis grossedentata via the TLR9-dependent anti-inflammatory pathway

Zhou, Hai-Yun; Gao, Shuang-Qi; Gong, Yusheng; Lin, Tong; Tong, Shuai; Xiong, Wei; Shi, Chunyu; Wang, Wenqing; Fang, Jianguo

doi:10.1016/j.jgar.2020.10.003

Cited by 15 publications

(11 citation statements)

References 35 publications

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“…Ampelopsis grossedentata is an important edible medicinal plant commonly called as vine tea belonging to the plant family Vitaceae and is mainly distributed in southern China. 15 Scientifically, high levels of flavonoids especially dihydromyricetin, myricetin and myricitrin were present in vine tea which were reported to possess antioxidant, 16 antibacterial, 17 antitumor, 18 anti-inflammatory, 19 neuroprotective, 20 antihypertensive and antidiabetic activities. 21 To the best of our knowledge the capacity of in vitro shoot culture of A. grossedentata in the production of secondary metabolites has not been studied until now.…”

Section: Introductionmentioning

confidence: 99%

Anticancer and antibacterial flavonoids from the callus of Ampelopsis grossedentata; a new weapon to mitigate the proliferation of cancer cells and bacteria

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Anticancer and antibacterial flavonoids from the callus of Ampelopsis grossedentata; a new weapon to mitigate the proliferation of cancer cells and bacteria

et al. 2022

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“…HSV-1 is primarily recognized by TLR2 and TLR3, which subsequently recruit adaptor proteins to activate signaling pathways that produce inflammatory cytokines and interferons [ 25 , 26 ]. A previous study showed that TLR2 and TLR3 mRNA levels are increased by HSV-1 infection in Vero cells [ 27 ]. Indeed, we also found that TLR2 and TLR3 expression levels were induced up to eight-fold in wild-type Vero cells after HSV-1 infection ( Figure 4 d).…”

Section: Resultsmentioning

confidence: 99%

Integrated CRISPR-Cas9 System-Mediated Knockout of IFN-γ and IFN-γ Receptor 1 in the Vero Cell Line Promotes Viral Susceptibility

Kim

Nowakowska

Kim

et al. 2022

IJMS

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The current pandemic and the possible emergence of new viruses urgently require the rapid development of antiviral vaccines and therapeutics. However, some viruses or newly generated variants are difficult to culture in common cell types or exhibit low viral susceptibility in vivo, making it difficult to manufacture viral vector-based vaccines and understand host–virus interactions. To address these issues, we established new cell lines deficient in both type I and type II interferon responses, which are essential for host immunity and interference with virus replication. These cell lines were generated by developing an integrated CRISPR-Cas9 system that simultaneously expresses dual-guide RNA cassettes and Cas9 nuclease in a single plasmid. Using this highly efficient gene-editing system, we successfully established three cell lines starting from IFN-α/β-deficient Vero cells, deleting the single interferon-gamma (IFNG) gene, the IFNG receptor 1 (IFNGR1) gene, or both genes. All cell lines clearly showed a decrease in IFN-γ-responsive antiviral gene expression and cytokine production. Moreover, production of IFN-γ-induced cytokines remained low, even after HSV-1 or HCoV-OC43 infection, while expression of the receptor responsible for viral entry increased. Ultimately, knockout of IFN-signaling genes in these cell lines promoted cytopathic effects and increased apoptosis after viral infection up to three-fold. These results indicate that our integrated CRISPR-Cas9-mediated IFNG- and IFNGR1-knockout cell lines promote virus replication and will be useful in viral studies used to design novel vaccines and therapies.

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“…Sample size was extremely variable and unable to be compared due to the differences in study designs. In terms of sample characteristics, 99 (72.26%) studies used Vero cells [ 2 , 3 , 6 , 8 - 10 , 14 - 20 , 23 , 25 , 26 , 28 , 30 - 34 , 36 , 39 - 42 , 44 - 47 , 50 , 51 , 53 - 56 , 58 - 64 , 66 - 70 , 72 , 74 , 76 , 77 , 79 , 82 , 83 , 85 - 87 , 89 - 95 , 97 - 108 , 111 - 113 , 115 - 118 , 120 , 122 , 123 , 125 - 130 , 132 , 133 , 135 , 137 , 138 ], 41 (29.93%) used human cell lines [ 7 , 11 , 12 , 16 , 19 , 24 , 29 , 31 , 36 , 38 , 47 , 49 , 52 - 56 , 61 , 62 , 67 ,…”

Section: Reviewmentioning

confidence: 99%

A Systematic Review of Second-Line Treatments in Antiviral Resistant Strains of HSV-1, HSV-2, and VZV

Lince¹,

DeMario²,

Yang³

et al. 2023

Cureus

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Drug-resistant variants of herpes simplex viruses (HSV) have been reported that are not effectively treated with first-line antiviral agents. The objective of this study was to evaluate available literature on the possible efficacy of second-line treatments in HSV and the use of second-line treatments in HSV strains that are resistant to first-line treatments. Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a final search was conducted in six databases on November 5, 2021 for all relevant literature using terms related to antiviral resistance, herpes, and HSV. Eligible manuscripts were required to report the presence of an existing or proposed second-line treatment for HSV-1, HSV-2, or varicella zoster virus (VZV); have full-text English-language access; and potentially reduce the rate of antiviral resistance. Following screening, 137 articles were included in qualitative synthesis. Of the included studies, articles that examined the relationship between viral resistance to first-line treatments and potential second-line treatments in HSV were included. The Cochrane risk-of-bias tool for randomized trials was used to assess risk of bias. Due to the heterogeneity of study designs, a meta-analysis of the studies was not performed. The dates in which accepted studies were published spanned from 2015-2021. In terms of sample characteristics, the majority (72.26%) of studies used Vero cells. When looking at the viruses on which the interventions were tested, the majority (84.67%) used HSV-1, with (34.31%) of these studies reporting testing on resistant HSV strains. Regarding the effectiveness of the proposed interventions, 91.97% were effective as potential managements for resistant strains of HSV. Of the papers reviewed, nectin in 2.19% of the reviews had efficacy as a second-line treatments in HSV, amenamevir in 2.19%, methanol extract in 2.19%, monoclonal antibodies in 1.46%, arbidol in 1.46%, siRNA swarms in 1.46%, Cucumis melo sulfated pectin in 1.46%, and components from Olea europeae in 1.46%. In addition to this griffithsin in 1.46% was effective, Morus alba L. in 1.46%, using nucleosides in 1.46%, botryosphaeran in 1.46%, monoterpenes in 1.46%, almond skin extracts in 1.46%, bortezomib in 1.46%, flavonoid compounds in 1.46%, andessential oils were effective in 1.46%, but not effective in 0.73%. The available literature reviewed consistently supports the existence and potentiality of second-line treatments for HSV strains that are resistant to first-line treatments. Immunocompromised patients have been noted to be the population most often affected by drug-resistant variants of HSV. Subsequently, we found that HSV infections in this patient population are challenging to manage clinically effectively. The goal of this systematic review is to provide additional information to patients on the potentiality of second-line treatment in HSV strains resistant to first-line treatments, especially those who are immunocompromised. All patients,...

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Anti-HSV-1 effect of dihydromyricetin from Ampelopsis grossedentata via the TLR9-dependent anti-inflammatory pathway

Cited by 15 publications

References 35 publications

Anticancer and antibacterial flavonoids from the callus of Ampelopsis grossedentata; a new weapon to mitigate the proliferation of cancer cells and bacteria

Anticancer and antibacterial flavonoids from the callus of Ampelopsis grossedentata; a new weapon to mitigate the proliferation of cancer cells and bacteria

Integrated CRISPR-Cas9 System-Mediated Knockout of IFN-γ and IFN-γ Receptor 1 in the Vero Cell Line Promotes Viral Susceptibility

A Systematic Review of Second-Line Treatments in Antiviral Resistant Strains of HSV-1, HSV-2, and VZV

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