Anti-Hyperglycemic Effects of Green Crab Hydrolysates Derived by Commercially Available Enzymes

Kang, Bouhee; Skonberg, Denise I.; Myracle, Angela D.

doi:10.3390/foods9030258

Cited by 15 publications

(9 citation statements)

References 57 publications

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“…36 Flavourzyme-treated green crab hydrolysates exhibited α-glucosidase inhibitory activity (IC 50 = 20.24 mg/ mL). 37 Sardine muscle hydrolyzed by alkaline protease showed a potent α-glucosidase inhibitory activity with an IC 50 value of 48.7 mg/mL. 38 Overall, SPPs generated by pepsin and bromelain in this work showed an effective α-glucosidase inhibitory potency, which further demonstrated the dual antidiabetic activity of SPPs.…”

Section: ■ Results and Discussionmentioning

confidence: 66%

“…The peptidic fraction (1 kDa) of Mucuna pruriens obtained by sequential hydrolysis of pepsin and pancreatin exhibited an inhibitory activity against α-glucosidase with an IC 50 value of 75 mg/mL . Flavourzyme-treated green crab hydrolysates exhibited α-glucosidase inhibitory activity (IC 50 = 20.24 mg/mL) . Sardine muscle hydrolyzed by alkaline protease showed a potent α-glucosidase inhibitory activity with an IC 50 value of 48.7 mg/mL .…”

Section: Results and Discussionmentioning

confidence: 99%

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Exploration of Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Peptides from Silkworm Pupae (Bombyx mori) Proteins Based on In Silico and In Vitro Assessments

Luo

et al. 2022

J. Agric. Food Chem.

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This study aimed at exploring dipeptidyl peptidase-IV (DPP-IV) inhibitory peptides from silkworm pupae proteins by in silico analysis and in vitro assessments. In silico analysis of 274 silkworm pupae proteomes indicated that DPP-IV inhibitory peptides can be released from silkworm pupae proteins. In vitro assessments revealed that pepsin and bromelain led to better production of DPP-IV inhibitory peptides from silkworm pupae protein. Notably, peptide fractions (<1 kDa) from pepsin-and bromelain-treated hydrolysates exhibited more potent DPP-IV inhibitory activities. Two novel DPP-IV inhibitory peptides (Leu-Pro-Pro-Glu-His-Asp-Trp-Arg and Leu-Pro-Ala-Val-Thr-Ile-Arg) were identified by LC-MS/MS with IC 50 values of 261.17 and 192.47 μM, respectively. Enzyme kinetics data demonstrated that these two peptides displayed a mixed-type DPP-IV inhibition mode, which was further validated by molecular docking data. Overall, in silico analysis combined with in vitro assessments can serve as an effective and rapid approach for discovery of DPP-IV peptides from silkworm pupae proteins.

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Section: ■ Results and Discussionmentioning

confidence: 66%

Section: Results and Discussionmentioning

confidence: 99%

Exploration of Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Peptides from Silkworm Pupae (Bombyx mori) Proteins Based on In Silico and In Vitro Assessments

Luo

et al. 2022

J. Agric. Food Chem.

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“…According to the method of Kang et al [ 30 ], HEMH solutions were added to ultrafiltration centrifuge tubes with 30 kDa, 10 kDa, and 3 kDa cut-off filters, and then centrifuged at 3000× g for 15 min. Peptide fractions labeled HEMH-I (MW < 3 kDa), HEMH-II (MW 3–10 kDa), HEMH-III (MW 10–30 kDa), and HEMH-IV (MW > 30 kDa) were obtained and freeze-dried for later use.…”

Section: Methodsmentioning

confidence: 99%

Hatched Eggshell Membrane Can Be a Novel Source of Antioxidant Hydrolysates to Protect against H2O2-Induced Oxidative Stress in Human Chondrocytes

Zhu

Ahn

et al. 2022

Antioxidants

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Natural antioxidants derived from agricultural by-products have great promise and ecological advantages in the treatment of oxidative stress-related diseases. The eggshell membrane (ESM) from hatched eggs, i.e., the hatched ESM, is a globally abundant agricultural byproduct, and its high-value utilization has been rarely studied compared to the well-studied ESM from fresh eggs. In this research, we systematically characterized the hatched ESM as a novel source of antioxidant hydrolysates and explored their potential role in H2O2-induced human chondrocytes. The results showed that the hatched ESM is a protein-rich fibrous mesh material with a significantly different structure and composition from those of fresh ESM. Enzymatic hydrolysis of hatched ESM can produce antioxidant hydrolysates rich in low molecular weight (MW) peptides, which mainly derived from the Lysyl oxidase homolog by Nano-LC-MS/MS analysis. The peptide fraction with MW < 3 kDa (HEMH-I) exhibited the highest DPPH radical scavenging, Fe2+-chelating, and Fe3+-reducing abilities. In H2O2-induced human SW1353 chondrocytes, HEMH-I treatment significantly increased the cell viability and ameliorated oxidative stress, inflammatory response, and cartilage matrix degradation by reducing the level of ROS, matrix metalloprotease 3 (MMP3), MMP13, and IL-6, and by promoting the expression of SOD and type II collagen, potentially through activating the cellular Keap1/Nrf2/HO-1 pathway. This study provides a theoretical basis for the value-added application of hatched ESM waste to produce antioxidant hydrolysates and indicates their potential as functional food and pharmaceuticals.

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“…Bioactive hydrolysates were developed from underutilized green crab by enzymatic proteolysis using Protamex. This hydrolysate exhibited more significant α-glucosidase inhibitory activity with the potential for treating type 2 diabetes (Kang et al, 2020).…”

Section: Crabmentioning

confidence: 97%

Novel marine bioactives: application in functional foods, nutraceuticals, and pharmaceuticals

Shahidi

Santhiravel

2022

Journal of Food Bioactives

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Functional food, nutraceutical, and pharmaceutical applications of natural products have gained growing attention as there is increasingly awareness of the association between bioactive compounds and improved health. Recently, food and biomedical scientists have focused more on marine resources to isolate bioactives since the marine ecosystem comprises unexploited resources with a wide range of organisms. Marine species produce a wide range of natural products, such as polysaccharides, peptides, polyunsaturated lipids, phenolic compounds, and pigments, with unique structures and diverse biological activities due to their extreme living environments. These active molecules reduce the risk of chronic disease and improve health by exhibiting antioxidant, anticancer, anti-inflammatory, antimicrobial, antidiabetic, anti-obesity, antihypertensive, cardioprotective, and neuroprotective activities. This review summarizes the recent discoveries of bioactive compounds from marine invertebrates (sponges, cnidarians, echinoderms, molluscs, ascidians, and crustaceans), fishes, seaweeds, and marine microorganisms and their potential for functional foods, nutraceuticals, and pharmaceuticals applications.

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Anti-Hyperglycemic Effects of Green Crab Hydrolysates Derived by Commercially Available Enzymes

Cited by 15 publications

References 57 publications

Exploration of Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Peptides from Silkworm Pupae (Bombyx mori) Proteins Based on In Silico and In Vitro Assessments

Exploration of Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Peptides from Silkworm Pupae (Bombyx mori) Proteins Based on In Silico and In Vitro Assessments

Hatched Eggshell Membrane Can Be a Novel Source of Antioxidant Hydrolysates to Protect against H2O2-Induced Oxidative Stress in Human Chondrocytes

Novel marine bioactives: application in functional foods, nutraceuticals, and pharmaceuticals

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