Anti-infective potential of hot-spring bacteria

Abstract

Aim and Background:Antibiotic resistance currently spans most of the known classes of natural and synthetic antibiotics; limiting our options for treatment of infections and demanding discovery of new classes of antibiotics. Much effort is being directed towards developing new antibiotics to overcome this problem. Success in getting novel chemical entities from microbial sources depends essentially on novelty of its habitat. The diversity of geographical location decides the type of micro-flora. In the past various terrestrial and aqueous microorganisms have provided several novel bioactive secondary metabolites of pharmaceutical importance. Hot-springs have not been as extensively exploited as other terrestrial resources. However, perseverance with such microbes augment the probability of getting novel bioactive compounds.Materials and Methods:Hot-springs soil samples were collected from Hot-springs in Maharashtra. Actinomycetes and other eubacteria were isolated from these soil samples by selective methods and purified. They were classified based on gram's nature and morphology. Six representative morphological strains were screened for their anti-infective potential by agar well diffusion method as reported by Nathan P. et al (1974). The bioactivity of the active microbes was confirmed.Results:Seventy three strains of bacteria encompassing eight actinomycetes, and 65 eubacteria were isolated and purified. Among the actives eubacteria PPVWK106001 showed broad spectrum antibacterial activity encompassing both gram positive and gram negative bacterial test models. The extract was active against resistant bacteria such as MRSA and VREs. Activity was very specific as there was no activity against fungi even at 100 fold concentration. The active principle was extractable in butanol.Conclusions:The study showed that Hot-springs exhibit diverse bacteria and it serves as potential reservoirs for bacteria of antimicrobial importance with diverse facet of activities. Thus Hot-springs microbes have ability to address issue of resistant bugs.

Identification, Characterization Enzymatic Antagonist Activity and Antimicrobial Resistance Profile of Bacteria Isolated From Brazilian Thermal Aquifer / Identificação, Caracterização Enzimática, Atividade Antagonista E Perfil De Resistência a Antimicrobianos De Bactérias Termotolerantes Isoladas Em Aquífero Termal Brasileiro

“…Members of the genus Bacillus are known to produce antimicrobial metabolites as antifungals and antibacterial (Bodhankar et al, 2017;Pandya et al, 2017;Boottanun et al, 2017). These findings corroborate the results from Pednekar et al (2011), where the authors concluded that most antimicrobials in bacilli, paenibacillus and streptomycetes are anti-Gram-positive bacteria. Also, Genomic analysis performed by Nascimento et al (2020) revealed the presence of several genes producing antimicrobial compounds in the B. megaterium STB1 strain, some homologous to those produced by B. subtilis related to antagonist activity and production of antifungal and antibacterial compounds.…”

Physicochemical and Microbial Diversity Analyses of Indian Hot Springs

“…The high arsenate-tolerant novel species Pannonibacter indica was reported from an Athamallik (Orissa, India) hot spring sediment ( Bandyopadhyay et al, 2013 ). Antimicrobial ability of bacterial strains isolated from a Maharashtra (India) hot spring was also reported ( Pednekar et al, 2011 ). A novel species, Thermus parvatiensis , isolated from the hot water spring of Manikaran, India, was reported to produce a thermostable enzyme (protease activity at 70°C) ( Dwivedi et al, 2015 ).…”

“…India harbors approximately 400 geothermal springs ( Pednekar et al, 2011 ). People bathe in these hot springs to cure diseases ( Pednekar et al, 2011 ; Narsing Rao et al, 2018 ). Culture-dependent microbial diversity analysis of Indian hot springs suggested that they harbor novel candidates and produce thermostable secondary metabolites.…”

Thermophilic Actinomycetes from Hot Water Spring Capable of Producing Enzymes of Industrial Importance

“…Sediment sample from hot spring, showed a good diversity of actinomycetes colonies while water sample had only two types. Several strains of actinomycetes have been previously reported from Hot-springs [18]. Thermophilic actinomycetes include the genera Streptomyces, Micromonospora, Actinomadura, Saccharomonospora and Thermoactinomyces [19].…”