Anti-infective potential of marine actinobacteria against carbapenem resistant <i>Klebsiella pneumoniae</i> ATCC 13882

Enmozhi, Sukanth Kumar; Vijayalakshmi, Ganesan; Kaari, Manigundan; Bharathi, S.; Venugopal, Gopikrishnan; Joseph, Jerrine; Radhakrishnan, Manikkam

doi:10.5958/0974-360x.2020.00646.0

Cited by 2 publications

(1 citation statement)

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“…A phylogenetic and molecular evolutionary analysis of BS-16 was conducted using software included in the MEGA version 418 package. The 16S rRNA sequence of the strains BS-16 was aligned () against corresponding nucleotide sequences of representatives of the genus Streptomyces retrieved from GenBank. – Evolutionary distance matrices were generated as described, and a phylogenetic tree was inferred by the Neighbor joining method. Tree topologies were evaluated by bootstrap analysis based on 1000 resembling of the neighbor joining data set. , …”

Section: Methodsmentioning

confidence: 99%

Exploring Antibacterial Properties of Bioactive Compounds Isolated from Streptomyces sp. in Bamboo Rhizosphere Soil

Sriragavi,

Sangeetha,

Santhakumar

et al. 2023

ACS Omega

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The increasing concern over multidrug resistance in pathogens has led to an ongoing search for novel antibiotics derived from soil actinobacteria. In this current investigation, actinobacteria were isolated from the rhizosphere of bamboo plants collected within the Megamalai forest of the Western Ghats in the Theni zone of Tamil Nadu, India. These actinobacteria were subjected to characterization, and their growth conditions were optimized to enhance the production of bioactive compounds. To assess antibacterial properties, the isolated Actinobacteria underwent testing using the agar plug method. The strain exhibiting notable antibacterial activity underwent further characterization through 16s rRNA gene sequencing and subsequent phylogenetic analysis. Employing response surface methodology (RSM), cultural conditions were fine-tuned. Bioactive compounds were extracted from the culture medium using ethyl acetate, and their antibacterial and antioxidant effects were evaluated through disc diffusion and DPPH radical scavenging methods, respectively. Ethyl acetate extracts were analyzed by using FT-IR and GC–MS techniques. In total, nine strains of Actinobacteria were isolated from the rhizosphere soil of bamboo. Among these, strain BS-16 displayed remarkable antibacterial activity against three strains: Staphylococcus aureus (19 mm), Bacillus subtilis (12 mm), and Streptococcus pyogenes (10 mm). This strain was identified as Streptomyces sp. The optimal conditions for bioactive compound production were determined as follows: malt extract (10 g), yeast extract (5 g), dextrose (5 g), pH 6.5, and temperature 30 °C. After a 7-day incubation period, the results showed a 6% increase in production. The ethyl acetate fraction derived from strain BS-16 exhibited dose-dependent antibacterial and antioxidant activities. FT-IR and GC–MS analyses revealed the presence of active compounds with antibacterial effects within the extract. Consequently, further investigation into the BS-16 strain holds promise for scaling up the production of bioactive compounds possessing antibacterial and antioxidant properties.

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