Anti-inflammatory Activities of Aqueous Extract from Radix Ophiopogon japonicus and Its Two Constituents

Kou, Junping; Sun, Yang; Lin, Yung‐Chieh; Cheng, Zhihong; Zheng, Wei; Yu, Boyang; Xu, Qiang

doi:10.1248/bpb.28.1234

Cited by 122 publications

(79 citation statements)

References 15 publications

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“…2B). These findings were consistent with our previous results (12) and confirmed that ruscogenin exerted significant antiinflammatory activity without affecting the cycloxygenase-2 (COX-2) pathway, which might be connected with inhibition of adhesion molecule expression or reduction of other inflammatory mediator release etc.…”

Section: Discussionsupporting

confidence: 93%

“…As described, ruscogenin had no obvious effect on the increase of PGE 2 in peritoneal fluid of mice (Fig. 2B) and the expression of cycloxygenase-2 (COX-2) in ECV304 cells (12), suggesting that some structural differences between ruscogenin and dexamethasone resulted in the different protein targets or signaling pathways. Further studies are required to clarify these differences.…”

Section: Discussionmentioning

confidence: 64%

“…Zymosan peritonitis was induced as we previously reported (12). Briefly, mice were administered orally with ruscogenin at doses of 0.3, 1, and 3 mg/ kg and dexamethasone at 3 mg/ kg; and then they were injected intraperitoneally with 1 mg zymosan A in 0.5 ml of sterile phosphate-buffered saline (PBS) 1 h later.…”

Section: Zymosan A-induced Peritonitis In Micementioning

confidence: 99%

“…Our previous studies also showed that ruscogenin significantly inhibited the adhesion of leukocytes to ECV304 cells activated by tumor necrosis factor-α (TNF-α), while it had no remarkable effect on leukocyte adherence to resting ECV304 cells and cell viability, and also inhibited leukocyte migration in vivo. Meanwhile, it did not down-regulate expression of cycloxygenase-2 (COX-2) mRNA induced by phorbol ester (PMA) in ECV304 cells (12,13). However, little is known about the anti-inflammatory mechanisms of ruscogenin and Radix Ophiopogon japonicus in endothelial responses to cytokines during inflammatory and vascular disorders.…”

mentioning

confidence: 98%

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Possible Mechanism of the Anti-inflammatory Activity of Ruscogenin: Role of Intercellular Adhesion Molecule-1 and Nuclear Factor-κB

Huang

Kou

Ma³

et al. 2008

J Pharmacol Sci

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Abstract. Ruscogenin (RUS), first isolated from Ruscus aculeatus, also a major steroidal sapogenin of traditional Chinese herb Radix Ophiopogon japonicus, has been found to exert significant anti-inflammatory and anti-thrombotic activities. Our previous studies suggested that ruscogenin remarkably inhibited adhesion of leukocytes to a human umbilical vein endothelial cell line (ECV304) injured by tumor necrosis factor-α (TNF-α) in a concentration-dependent manner. Yet the underlying mechanisms remain unclear. In this study, the in vivo effects of ruscogenin on leukocyte migration and celiac prostaglandin E 2 (PGE 2 ) level induced by zymosan A were studied in mice. Furthermore, the effects of ruscogenin on TNF-α-induced intercellular adhesion molecule-1 (ICAM-1) expression and nuclear factor-κB (NF-κB) activation were also investigated under consideration of their key roles in leukocyte recruitment. The results showed that ruscogenin significantly suppressed zymosan A-evoked peritoneal total leukocyte migration in mice in a dose-dependent manner, while it had no obvious effect on PGE 2 content in peritoneal exudant. Ruscogenin also inhibited TNF-α-induced over expression of ICAM-1 both at the mRNA and protein levels and suppressed NF-κB activation considerably by decreasing NF-κB p65 translocation and DNA binding activity. These findings provide some new insights that may explain the possible molecular mechanism of ruscogenin and Radix Ophiopogon japonicus for the inhibition of endothelial responses to cytokines during inflammatory and vascular disorders.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 64%

Section: Zymosan A-induced Peritonitis In Micementioning

confidence: 99%

mentioning

confidence: 98%

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Possible Mechanism of the Anti-inflammatory Activity of Ruscogenin: Role of Intercellular Adhesion Molecule-1 and Nuclear Factor-κB

Huang

Kou

Ma³

et al. 2008

J Pharmacol Sci

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“…The anti-inflammatory activity was evaluated by using model of xylene-induced ear edema in mice according to Kou (Kou et al, 2005) with some modifications. The animals were randomly allocated to groups GI -IV, each group consisting of five mice, as follows: GI received only distilled water, (positive control); GII received Carbopol empty gel (negative control); GIII received gel containing V. doniana at 2.5% and GIV the pure extract of V. doniana at 10mg/ml, applied topically immediately after inducing edema.…”

Section: Topical Anti-inflammatory Activitymentioning

confidence: 99%

Preliminary Evaluation of the Wound Healing Effect of <em>Vitex Doniana</em> Sweet (Verbenaceae) in Mice

Amegbor

Metowogo²,

Eklu-Gadégbéku³

et al. 2012

Afr. J. Trad. Compl. Alt. Med.

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Vitex doniana is traditionally used in Togo to treat various diseases including wounds. The aim of this work was to evaluate the efficiency of Vitex doniana on cutaneous wound healing. Wounds were induced in ICR mice divided into four groups as following: Group I received carbopol 974P NF empty gel, Groups II and III were treated topically with carbopol gel containing 2.5% and 5% of Vitex doniana extract. Group IV received Betadine® 10% as standard drug. The efficacy of treatment was evaluated by planimetry and histological analysis. We secondary used the gel containing Vitex doniana at 2.5% and the pure extract at 10 mg/ml on the model of ear edema induced by xylene. Skin toxicity test was performed with the gel containing Vitex doniana at 5% and the pure extract at 30 mg/ml. Vitex doniana at 5% and 2.5% provided better wound contraction (91.14% and 86.38%) at day 12 post-excision when compared to control (51.15%). The results of histological evaluation supported the outcome of excision wound model. Moreover Vitex doniana inhibited significantly edema induced by xylene when compared to control (p< 0.05). In skin toxicity test, no abnormal symptoms were developed over 14 day-time period. Vitex doniana inhibits the topical inflammation and accelerate cutaneous wound repair.

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Comparison of anti‐inflammatory activities of ruscogenin, a major steroidal sapogenin from Radix Ophiopogon japonicus, and Its succinylated derivative, RUS‐2HS

Huang

Kou

Liu

et al. 2008

Drug Development Research

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Ruscogenin (RUS), first isolated from Ruscus aculeatus, is also a major steroidal sapogenin of the traditional Chinese herb Radix Ophiopogon japonicus. It has robust anti-inflammatory activities. In previous studies, a ruscogenin affinity column, derived from succinylated ruscogenin (RUS-2HS), was used to purify an antibody of ruscogenin. A ruscogenin affinity column can also be used to explore its protein targets. However, until now there have been no related pharmacological reports about ruscogenin derivatives. Whether the activity groups of ruscogenin have been blocked during the derivation process remains unknown. The present study was performed to compare the anti-inflammatory activities in vitro of RUS-2HS and ruscogenin. Both compounds reduced tumor necrosis factor-a (TNF-a)-induced adhesion of human pro-myelocytic leukemia cells (HL-60) to endothelial ECV304 cells with IC 50 values of 6.90 nM and 7.45 nM, respectively. They were also inhibited overexpression of ICAM-1 in ECV304 cells at the mRNA level as evaluated by real-time PCR and at the protein level evaluated by flow cytometry with similar potency. Such data demonstrate that the functional groups of ruscogenin were not blocked by derivation, suggesting further use of the ruscogenin affinity column for target investigation. Meanwhile, RUS-2HS was found to have remarkable anti-inflammatory activity for the first time, indicating it would be a new lead compound with improved bioavailability. Drug Dev Res 69: 196-202, 2008.

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Anti-inflammatory Activities of Aqueous Extract from Radix Ophiopogon japonicus and Its Two Constituents

Cited by 122 publications

References 15 publications

Possible Mechanism of the Anti-inflammatory Activity of Ruscogenin: Role of Intercellular Adhesion Molecule-1 and Nuclear Factor-κB

Possible Mechanism of the Anti-inflammatory Activity of Ruscogenin: Role of Intercellular Adhesion Molecule-1 and Nuclear Factor-κB

Preliminary Evaluation of the Wound Healing Effect of <em>Vitex Doniana</em> Sweet (Verbenaceae) in Mice

Comparison of anti‐inflammatory activities of ruscogenin, a major steroidal sapogenin from Radix Ophiopogon japonicus, and Its succinylated derivative, RUS‐2HS

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