Anti-inflammatory Activities Verification of Vaccinum oldhami Fruit Ethanol Extracts on RAW 264.7

Abstract

The purpose of this study was to investigate the role of the Vaccinum oldhami fruit extract as a cosmetic additive. As a result of having macrophage (RAW 264.7) measured a cell toxicity effects of 70% ethanol extract from Vaccinum oldhami fruit, it shown 118% with toxicity at 500 μg/ml concentration. In nitric oxide synthesis inhibition effect, 70% ethanol extracts from Vaccinum oldhami fruit shown 47.3% at 1,000 μg/ml concentration. The iNOS, COX-2 protein expression inhibitory effect by western blot of 70% ethanol extract from Vaccinum oldhami fruit was decreased by 36.13%, 29.61% at 500 μg/ml concentration. And iNOS, COX-2 mRNA expression inhibitory effect by reverse-transcription-PCR of 70% ethanol extract from Vaccinum oldhami fruit was decreased by 62.25%, 90.07% at 500 μg/ml concentration. All these finding that extract from Vaccinum oldhami fruit could prove that their have effects anti-inflammatory efficacy. And extract from Vaccinum oldhami fruit has potential as a cosmetic ingredients. Key words

Growing environment characteristics and vegetation structure ofVaccinium OldhamiiMiq. native habitats in Korea

“…There have only been a small number of studies on V. oldhamii, which were mostly focused on its pharmaceutical uses, such as the antioxidant effect of its fruits (Chae et al 2010), physiological activity, antimicrobial effects (Chae et al 2012) and anti-inflammatory effects (Lee et al 2017). One study investigated the morphology of its leaves and fruits (Kim et al 2012).…”