Anti-inflammatory activity of Cymbopogon citratus leaves infusion via proteasome and nuclear factor-κB pathway inhibition: Contribution of chlorogenic acid

Abstract

inflammatory activity of Cymbopogon citratus leaves infusion via proteasome and nuclear factor-κB pathway inhibition: contribution of chlorogenic acid, Journal of Ethnopharmacology, http://dx.doi.org/10. 1016/j.jep.2013.03.077 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Activation of nuclear factor-țB, a master regulator of inflammation, was investigated by Western blot and gene reporter assay. Proteasome activity was assessed using a fluorogenic peptide.Results: Cymbopogon citratus extract and its polyphenols inhibited the cytokine production on human macrophages. This supports the anti-inflammatory activity of Cy polyphenols in physiologically relevant cells. Concerning the effect on the activation of nuclear factor (NF)-țB pathway, the results pointed to an inhibition of LPS-induced NF-țB activation by Cy and PFs.Chlorogenic acid was identified, by HPLC/PDA/ESI-MS n , as the main phenolic acid of the Cy infusion, and it demonstrated to be, at least in part, responsible by that effect. Additionally, it was verified for the first time, that Cy and PFs inhibited the proteasome activity, a complex that controls NF-țB activation, having CGA a strong contribution. Conclusions:The results evidenced, for the first time, the anti-inflammatory properties of Cymbopogon citratus through proteasome inhibition and, consequently NF-țB pathway and cytokine expression. Additionally, Cy polyphenols, in particular chlorogenic acid, were highlighted as bio-active compounds.

Chlorogenic Acid Combined with Lactobacillus plantarum 2142 Reduced LPS-Induced Intestinal Inflammation and Oxidative Stress in IPEC-J2 Cells

“…For instance, the anti-inflammatory effect of an extract from Cymbopogon citratus (CC) in human macrophages was demonstrated. The results showed that the leaf extract of CC inhibits LPS-induced cytokine expression through NF-κB pathway [30]. In addition, Artemisia annua extract was applied on Caco-2 cells in order to reduce intestinal inflammation.…”

Essential oils rich in monoterpenes are unsuitable as additives to boar semen extender

“…Cymbopogon citratus (also known as lemongrass) leaves' infusion has been used in folk medicine of many tropical and subtropical regions to treat gastric disturbances and inflammatory conditions (Sagradas et al, 2015). Francisco et al (2013) showed the anti-inflammatory properties of C. citratus through proteasome inhibition and consequently NF-κB pathway and cytokine expression with Cy polyphenols, in particular chlorogenic acid as a bioactive compound. Francisco et al (2013) showed the anti-inflammatory properties of C. citratus through proteasome inhibition and consequently NF-κB pathway and cytokine expression with Cy polyphenols, in particular chlorogenic acid as a bioactive compound.…”

Gastroprotective effect of Cymbopogon citratus infusion on acute ethanol-induced gastric lesions in rats

“…It is also recommended in folk medicine to treat gastric disturbances, stomachache, gastritis and ulcers (De Albuquerque et al, 2007;Novais et al, 2004;Tene et al, 2007). Studies on C. citratus leaves infusions and decoctions have shown antioxidant and anti-inflammatory activities (Carbajal et al, 1989;Cheel et al, 2005;Figueirinha et al, 2010Figueirinha et al, , 2008Francisco et al, 2013Francisco et al, , 2011Habana, 2001;Pereira et al, 2009) and revealed the presence of several polyphenols, including tannins, phenolic acids and flavonoids (Cheel et al, 2005;Figueirinha et al, 2008;Francisco et al, 2014;Marques and Farah, 2009;Pereira et al, 2009). Those properties may be indicative of the potential benefit in the gastrointestinal tract, mainly on gastric mucosal injury generated by oxidative damage.…”