Chlorogenic Acid Combined with Lactobacillus plantarum 2142 Reduced LPS-Induced Intestinal Inflammation and Oxidative Stress in IPEC-J2 Cells

Abstract

This study was carried out to investigate protective effect of chlorogenic acid against lipopolysaccharide-induced inflammation and oxidative stress in intestinal epithelial cells. As a marker of inflammatory response, IL-6, IL-8, TNF-α mRNA and protein levels, furthermore, COX-2 mRNA level were followed up. Intracellular redox status and extracellular H2O2 level were also monitored by two fluorescent assays (DCFH-DA, Amplex Red). Moreover, the effect of gut microbiota metabolites in the above mentioned processes was taken into account in our model using Lactobacillus plantarum 2142 bacterial strain. Our data revealed that chlorogenic acid had significant lowering effect on the inflammatory response. Treatment with chlorogenic acid (25–50 μM) significantly decreased gene expression and concentration of proinflammatory cytokines IL-6 and IL-8 compared to LPS-treated cells. COX-2 and TNF-α mRNA levels were also reduced. Furthermore, chlorogenic acid reduced the level of reactive oxygen species in IPEC-J2 cells. Simultaneous application of chlorogenic acid and Lactobacillus plantarum 2142 supernatant resulted protective effect against LPS-induced inflammation and oxidative stress as well.

Tracing the Anti‐Inflammatory Mechanism/Triggers of d‐Allulose: A Profile Study of Microbiome Composition and mRNA Expression in Diet‐Induced Obese Mice

“…Lactobacillus rhamnosus GG and Lactobacillus casei DN ‐ 114 001 have been found to decrease the degradation of IĸB and thereby minimize the production of the inflammatory cytokine . L. casei CRL 431, Lactobacillus paracasei CNCM I‐4034, L. rhamnosus Lc705, and Lactobacillus plantarum 2142 have been found to dramatically reduce the production of inflammatory cytokines in in vitro and obese mouse model . Coprococcus are producers of the anti‐inflammatory SCFA butyrate, and experimental colonization with Akkermansia implicated members of the genera in the immune tolerance of commensal gut microbes .…”

Leonurus sibiricus root extracts decrease airway remodeling markers expression in fibroblasts

“…apples, bananas, coffee, beans) [51]. Chlorogenic acid has been suggested to alleviate lipopolysaccharide (LPS)-induced inflammation and downregulate cyclooxygenase-2 expression -the feature attributed to inflammation in many chronic diseases [52][53][54]. Most of the natural products containing CGAs demonstrate anti-inflammatory effects, and it has been proposed that CGA may be a noticeable anti-oxidative agent [55,56].…”

Effects of phytogenic feed additives on cellular oxidative stress and inflammatory reactions in intestinal porcine epithelial cells1

“…Therefore, that cell line has become an increasingly important model in recent years ( Berschneider, 1989 ; Schierack et al, 2006 ). In several studies, IPEC-J2 were successfully employed for research on intestinal inflammation and oxidative stress response ( Schierack et al, 2006 ; Farkas et al, 2014 ; Palócz et al, 2016 ), making them an ideal cell line to study the response of intestinal epithelial cells to PFA. In the current study, we demonstrate inflammation-restricting and antioxidative properties of the complex PFA D-DC in the IPEC-J2 cell culture model and provide detailed knowledge about its mode of action.…”