Anti-inflammatory activity of Devil’s claw in vitro systems and their active constituents

Gyurkovska, Valeriya; Alipieva, Kalina; Maciuk, Alexandre; Dimitrova, Petya; Ivanovska, Nina; Haas, Christiane; Bley, Thomas; Georgiev, Milen I.

doi:10.1016/j.foodchem.2010.08.056

Cited by 90 publications

(65 citation statements)

References 27 publications

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“…Verbascoside (known as acteoside, kusaginin or orobanchin) exhibits a wide spectrum of biological activities, including antileukemic and cytotoxic activity against a murine cell line (Pettit et al 1990). It demonstrates anti-inflammatory activity through inhibition of cyclooxygenase-2 (COX-2) expression and inhibition of complement activity in human serum (Gyurkovska et al 2011), and also possesses diuretic (Herbert et al 1991) and antibacterial properties (Pennacchio 2005). In turn, isoverbascoside has been found to demonstrate antibacterial and anti-inflammatory activities (Shikanga et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Shoot organogenesis, molecular analysis and secondary metabolite production of micropropagated Rehmannia glutinosa Libosch.

Piątczak

Kuźma

Sitarek

et al. 2014

Plant Cell Tiss Organ Cult

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This study is the first description of a procedure for shoot organogenesis from calli derived from various seedling explants. The frequency of shoot induction and number of shoots per explant were measured on Murashige and Skoog (MS) medium with 0.2-3.0 mg L -1 6-benzylaminopurine (BAP) alone or combined with auxin [0.1 mg L -1 indole-3-acetic acid (IAA) or 1-naphthalenacetic acid]. The highest organogenic response where 90 % of explants formed a regenerative callus with a mean number of nine shoots was achieved after 6 weeks of culture when hypocotyl explants and 1.0 mg L -1 BAP was used together with 0.1 mg L -1 IAA. The shoots regenerated from hypocotyl-derived calli were rooted for 6 weeks on MS medium lacking growth regulators or containing auxin (IAA or indole-3-butyric acid). The plantlets were successfully acclimatized in a greenhouse. Micropropagated plants subjected to random amplified polymorphic DNA and inter simple sequence repeat (ISSR) marker based profiling revealed a uniform banding pattern identical with that of donor plants. The iridoid and phenylethanoid glycoside content of the organogenic callus culture and in vitro-raised plants was determined using UHPLC. In the tested samples catalpol, aucubin, harpagid, harpagoside, verbascoside, isoverbascoside and traces of loganin and catalposide were identified. The leaves produced the highest levels of catalpol (43-45 mg g -1 DW). Organogenic callus and 6-week-old in vitro grown plantlets were characterized by the highest accumulation of aucubin, verbascoside and isoverbascoside. The work also demonstrated the regenerative ability of hypocotyl-derived calli and production of bioactive metabolites to be stable for 4 years of culture.

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Section: Introductionmentioning

confidence: 99%

Shoot organogenesis, molecular analysis and secondary metabolite production of micropropagated Rehmannia glutinosa Libosch.

Piątczak

Kuźma

Sitarek

et al. 2014

Plant Cell Tiss Organ Cult

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“…The twenty seven compounds consisting up to 80% of the 14.0%, respectively). The third cluster enclosed accession of Naghan, and displayed high myrcene and α-thujene contents (15.9% and 13.4%, respectively).…”

Section: Resultsmentioning

confidence: 98%

“…Also, the yield of the oils extracted from other species was: 0.18% from Stachys setifera ssp. Iranica [31] , 0.18% from Stachy chrysantha [15] , and 0.12% from Stachy candida [14] . In other study, Radulovic et al reported that the yield of the obtained essential oils from Balkan Peninsula endemics species (Stachys germanica ssp.…”

Section: Discussionmentioning

confidence: 99%

“…The inflorescences (0.05-0.10 kg) of wild populations of S. lavandulifolia Vahl were collected at the early flowering stage on [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] June 2010 (During 20 day's period). The samples of the plants were identified by regional floras and authors with floristic and taxonomic references [3] , and voucher specimens were deposited at the Herbarium of Agriculture and Natural Resources Researches Centre of Chaharhal va Bakhtiari province, Iran (No.…”

Section: Plantsmentioning

confidence: 99%

“…Anti-inflammatory, antioxidant, antibacterial and anti-leishmania activities and effect reduced primary dysmenorrheal of S. lavandulifolia Vahl extract and essential oil have been shown in several pharmacological studies [11][12][13] . Previous investigations revealed that the main iridoid components of Stachys species, such as aucubin, harpagide and harpagoside, exert anti-inflammatory activities, as demonstrated in cellular systems of arachidonic acid metabolism [14,15] . Even though Stachys is one of the largest members of the Lamiaceae, the essential oil chemistry of the genus has not been studied in much detail.…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical composition of the essential oil of different populations of Stachys lavandulifolia Vahl

Pirbalouti

Mohammadi

2013

Asian Pacific Journal of Tropical Biomedicine

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Harpagide Inhibits Microglial Activation and Protects Dopaminergic Neurons as Revealed by Nanoelectrode Amperometry^†

et al. 2021

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Parkinson's disease (PD) is one of the most common neurogenerative diseases (NDDs), characterized as less neurotransmitter release and loss of dopaminergic (DAergic) neurons with microglial inflammatory response as a key player. Natural product harpagide with anti-inflammatory function is a potential therapeutic drug of PD, but its role towards microglial activation and inflammation-mediated neuronal injury remained unsure. In this work, taking advantage of nanoelectrode amperometry with high temporal-spatial resolution, we used nanowire electrodes (NWEs) to monitor intracellular reactive oxygen species (ROS) level and carbon fiber nanoelectrodes (CFNEs) to detect synaptic dopamine exocytosis, to explore the effect of harpagide in modulating microglial inflammatory reaction and protecting DAergic neurons in neuron-microglia co-culture system. The results indicate that harpagide inhibits microglia from activation induced by LPS/IFN-γ and generation of ROS, therefore reduces inflammation-mediated neural injury and maintains dopamine exocytosis function. These conclusions establish that harpagide possesses promising avenues for preventive or therapeutic interventions against PD and other NDDs.

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Anti-inflammatory activity of Devil’s claw in vitro systems and their active constituents

Cited by 90 publications

References 27 publications

Shoot organogenesis, molecular analysis and secondary metabolite production of micropropagated Rehmannia glutinosa Libosch.

Shoot organogenesis, molecular analysis and secondary metabolite production of micropropagated Rehmannia glutinosa Libosch.

Phytochemical composition of the essential oil of different populations of Stachys lavandulifolia Vahl

Harpagide Inhibits Microglial Activation and Protects Dopaminergic Neurons as Revealed by Nanoelectrode Amperometry^†

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Anti-inflammatory activity of Devil’s claw in vitro systems and their active constituents

Cited by 90 publications

References 27 publications

Shoot organogenesis, molecular analysis and secondary metabolite production of micropropagated Rehmannia glutinosa Libosch.

Shoot organogenesis, molecular analysis and secondary metabolite production of micropropagated Rehmannia glutinosa Libosch.

Phytochemical composition of the essential oil of different populations of Stachys lavandulifolia Vahl

Harpagide Inhibits Microglial Activation and Protects Dopaminergic Neurons as Revealed by Nanoelectrode Amperometry†

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Product

Resources

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Harpagide Inhibits Microglial Activation and Protects Dopaminergic Neurons as Revealed by Nanoelectrode Amperometry^†