Anti-Inflammatory Activity ofLactobacilluson Carrageenan-Induced Paw Edema in Male Wistar Rats

Abstract

Introduction. Lactobacillus casei and Lactobacillus acidophilus were used to assess the anti-inflammatory properties in carrageenan induced acute inflammatory model. Materials and Methods. Diclofenac sodium was used as standard drug at concentration of 150 mg/kg of body weight. Culture of Lactobacillus 2 × 107 CFU/ml was given orally. Edema was induced with 1% carrageenan to all the groups after one hour of the oral treatments. Paw thickness was checked at t = 1, 2, 3, 4, 5, and 24 hours. Stair climbing score and motility score were assessed at t = 24 hours. Cytokines assay for IL-6, IL-10, and TNF-α was performed on serum samples. Results. Lactobacillus showed a statistically significant decrease in paw thickness at P < 0.001. L. acidophilus and L. casei decreased by 32% and 28% in paw thickness. They both significantly increased the stair climbing and motility score. Lactobacillus treatment significantly downregulated IL-6 and TNF-α while upregulated IL-10 at P < 0.0001. Conclusion. L. casei and L. acidophilus significantly decreased the inflammatory reactions induced by carrageenan. This study has also proposed that Lactobacillus ameliorated the inflammatory reaction by downregulating the proinflammatory cytokines pathway.

Turkish Scorzonera Species Extracts Attenuate Cytokine Secretion via Inhibition of NF-κB Activation, Showing Anti-Inflammatory Effect in Vitro

“…Carrageenan-induced hind paw edema acute inflammation test model is biphasic. First phase starts with the release of histamine, serotonin, and kinins, while the second phase is related to the release of prostaglandins like substances [25]. All of the tested Scorzonera extracts were found be inactive in serotonin-induced hind paw edema model [23].…”

Probiotics protect mice from CoCrMo particles-induced osteolysis

“…Lactobacillus and its important species, such as L. casei and Lactobacillus acidophilus, have been used against many pathological and disease conditions. 31 Lactobacillus has been assessed for its immune-modulatory properties in various experiments. 31,32 In an ovariectomized (Ovx) mouse menopausal model, Britton et al found that Lactobacillus reuteri treatment significantly protected Ovx mice from bone loss.…”

Screening, Characterization, and in Vitro Evaluation of Probiotic Properties of Lactobacillus Strains

“…Probiotics have recently emerged as the most powerful food grade microbial agents comprise the ability to express numerous health promoting functions of considerable commercial value and therapeutic potential. These are live microorganisms, when administered in adequate amounts confer several health benefits on the host [1], which includes improving cholesterol assimilation [2], lactose tolerance [3], anti-inflammatory [4], antioxidative [5], anticancer [6], antidiarrheal [7], antiallergic [8], antidiabetic [9], antihypertensive effects [10], controlling obesity [11], and also helps in mineral absorption [12]. Probiotics received generally-regarded-as-safe status, due to its safety profile.…”