Anti-inflammatory activity of SMP30 modulates NF-κB through protein tyrosine kinase/phosphatase balance

Jung, Kyung Jin; Lee, Eun Kyeong; Kim, Su Jin; Song, Chang Woo; Maruyama, Naoki; Ishigami, Akihito; Kim, Nam Deuk; Im, Dong‐Soon; Yu, Byung Pal; Chung, Hae Young

doi:10.1007/s00109-014-1219-1

Cited by 20 publications

(16 citation statements)

References 45 publications

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“…It is hypothesized that the activation of SIRT1 promotes the deacetylation of Nrf2, improving the stability of this transcription factor [ 21 , 22 ]. The senescence marker protein-30 (SMP30), expressed mainly in the liver and kidney, is considered a biomarker of aging process, since its levels decrease with age [ 23 , 24 ]. It is suggested that SMP30 is able to suppress oxidative stress and consequently decrease NFkB levels, therefore, the decrease of SMP30 protein with age leads to an increase of oxidative damage and inflammatory response, which promotes aging [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Tucum-do-Cerrado (Bactris setosa Mart.) May Promote Anti-Aging Effect by Upregulating SIRT1-Nrf2 Pathway and Attenuating Oxidative Stress and Inflammation

Cunha

Arruda

2017

Nutrients

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Aging may be related to oxidative damage accumulation and a low-grade inflammation, both responses are modulated by iron and phytochemicals. This study investigated the effect of tucum-do-cerrado (Bactris setosa Mart.) consumption on the expression of sirtuins (SIRT 1 and 3) and senescence marker protein-30 (SMP30), and on the redox and inflammatory responses, in adult rats supplemented or not with dietary iron. Male Wistar rats were treated for 12 weeks with: control diet (CT); iron enriched-diet (+Fe); control diet + 15% tucum-do-cerrado (Tuc); or iron enriched-diet + 15% tucum-do-cerrado (Tuc + Fe). Iron supplementation (+Fe) increased liver, spleen and intestine iron levels, transferrin saturation, serum iron, serum TNF-α and IL-6 levels, hepatic carbonyl content and and superoxide dismutase (SOD) activity, hepatic Nrf2 protein and Nqo1 mRNA levels and decreased the renal Sirt1 mRNA levels in relation to CT group. Tucum-do-cerrado consumption (Tuc) increased hepatic SOD activity, Nrf2 and SIRT1 mRNA and protein contents, and Nqo1 mRNA levels, while it decreased the renal SOD activity compared with the CT diet. The consumption of tucum-do-cerrado associated with the iron-enriched diet (Tuc + Fe) increased the iron levels in tissues and serum transferrin saturation, compared to the CT diet, while promoting a decrease in hepatic carbonyl and renal malondialdehyde levels, marginally reducing serum IL-6 levels, and increasing hepatic SIRT1 protein content, renal Sirt1 and hepatic Nrf2 mRNA levels, compared to the +Fe group. None of the treatments altered Smp30 mRNA levels. The results suggest that tucum-do-cerrado consumption might promote an anti-aging effect by increasing SIRT1 expression, which may enhance Nrf2 mRNA and protein levels and its downstream pathway, which in turn decrease oxidative damage to proteins and the levels of inflammatory cytokines (IL-6 and TNF-α), induced by iron excess.

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Section: Introductionmentioning

confidence: 99%

Tucum-do-Cerrado (Bactris setosa Mart.) May Promote Anti-Aging Effect by Upregulating SIRT1-Nrf2 Pathway and Attenuating Oxidative Stress and Inflammation

Cunha

Arruda

2017

Nutrients

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“…In these studies, the body weight, plasma IL-6 levels, and plasma LDL levels were higher in the db/db mice than in the lean mice, and these were improved after stem cell replacement, suggesting that these parameters were related to the expression of SMP30 in the liver. SMP30 has been shown to modulate the balance of protein tyrosine kinase and protein tyrosine phosphates, and also to regulate NF-κB related inflammatory activity [ 17 ]. One report showed that, 30 min after glucose administration, blood glucose levels rose higher and insulin levels fell lower in SMP30-deficient mice than in wild-type mice [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

“…The relationship between SMP30 expression and oxidative stress is critical for understanding the mechanisms of senescence. Moreover, the activities of inflammatory NF-kB are higher in SMP30-deficient mice, suggesting that SMP30 prevents oxidative stress and chronic inflammation [ 17 ]. Indeed, one report indicates that high levels of oxidative stress caused abnormal plasma lipid metabolism and hepatic lipid accumulation in the SMP30 and superoxide dismutase 1-deficient mouse [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

Stem Cell Replacement Improves Expression of SMP30 in db/db Mice

Guo

Taketani

et al. 2015

IJMS

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We have previously reported that replacing bone marrow stem cells may improve hyperglycemia and oxidative stress in db/db mice, a type 2 diabetic mouse model. Senescence marker protein 30 (SMP30) is an antioxidant protein that decreases with aging. However, it has not been clear whether SMP30 decreases in the livers of obese mice, and whether stem cell replacement would improve SMP30 expression in the liver. Bone marrow stem cells of db/db mice were replaced with the bone marrow stem cells of C57BL/6 mice. Plasma cytokine and insulin levels were measured, and glycogen content, expression of SMP30, and fibrosis in the liver were assessed. Our results showed that stem cell replacement increased the expression of SMP30 in the liver, resulting from decreased plasma inflammation cytokines and hyperinsulinemia in db/db mice. This is the first report that stem cell replacement increased the expression of SMP30 in the liver, and may help prevent fibrosis in the liver of db/db mice.

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“…Mouse lung tissues were obtained from the Aging Tissue Bank (Pusan, Korea). Senescence marker protein (SMP)30 Y/+ and SMP30 Y/- mice were described previously [ 16 ]. Total RNA was purified using the RNeasy Mini Kit (Qiagen), according to the manufacturer's recommended protocol.…”

Section: Methodsmentioning

confidence: 99%

Oligonol promotes anti-aging pathways via modulation of SIRT1-AMPK-Autophagy Pathway

et al. 2016

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BACKGROUND/OBJECTIVESOligonol, mainly found in lychee fruit, is an antioxidant polyphenolic compound which has been shown to have anti-inflammatory and anti-cancer properties. The detailed mechanisms by which oligonol may act as an anti-aging molecule have not been determined.MATERIALS/METHODSIn this study, we evaluated the ability of oligonol to modulate sirtuin (SIRT) expression in human lung epithelial (A549) cells. Oligonol was added to A549 cells and reactive oxygen species production, mitochondrial superoxide formation, and p21 protein levels were measured. Signaling pathways activated upon oligonol treatment were also determined by western blotting. Furthermore, the anti-aging effect of oligonol was evaluated ex vivo in mouse splenocytes and in vivo in Caenorhabditis elegans.RESULTSOligonol specifically induced the expression of SIRT1, whose activity is linked to gene expression, metabolic control, and healthy aging. In response to influenza virus infection of A549 cells, oligonol treatment significantly up-regulated SIRT1 expression and down-regulated viral hemagglutinin expression. Oligonol treatment also resulted in the activation of autophagy pathways and the phosphorylation of AMP-activated protein kinase (AMPK). Furthermore, oligonol-treated spleen lymphocytes from old mice showed increased cell proliferation, and mRNA levels of SIRT1 in the lungs of old mice were significantly lower than those in the lungs of young mice. Additionally, in vivo lethality assay revealed that oligonol extended the lifespan of C. elegans infected with lethal Vibrio cholerae.CONCLUSIONSThese data demonstrated that oligonol may act as an anti-aging molecule by modulating SIRT1/autophagy/AMPK pathways.

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Anti-inflammatory activity of SMP30 modulates NF-κB through protein tyrosine kinase/phosphatase balance

Cited by 20 publications

References 45 publications

Tucum-do-Cerrado (Bactris setosa Mart.) May Promote Anti-Aging Effect by Upregulating SIRT1-Nrf2 Pathway and Attenuating Oxidative Stress and Inflammation

Tucum-do-Cerrado (Bactris setosa Mart.) May Promote Anti-Aging Effect by Upregulating SIRT1-Nrf2 Pathway and Attenuating Oxidative Stress and Inflammation

Stem Cell Replacement Improves Expression of SMP30 in db/db Mice

Oligonol promotes anti-aging pathways via modulation of SIRT1-AMPK-Autophagy Pathway

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