Anti-inflammatory activity of the ethanol extract of Dictamnus dasycarpus leaf in lipopolysaccharide-activated macrophages

Abstract

BackgroundDictamnus dasycarpus is widely used as a traditional remedy for the treatment of eczema, rheumatism, and other inflammatory diseases in Asia. The current study investigates the molecular mechanism of anti-inflammatory action of the ethanol extract of Dictamnus dasycarpus leaf (DE) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.MethodsNitric oxide (NO) production was assessed by Griess reaction and the mRNA and protein expressions of pro inflammatory cytokines, transcription factor, and enzymes were determined by real-time RT-PCR and immunoblotting analysis.ResultsDE (0.5 and 1 mg/mL) suppressed the NO production by 10 and 33%, respectively, compared to the untreated group in LPS-stimulated RAW 264.7 cells. DE (0.5 and 1 mg/mL) reduced the mRNA expression of key transcription factor nuclear factor-κB by 7 and 24%, respectively compared to the untreated group in LPS activated macrophage. The pro inflammatory cytokines such as tumor necrosis factor α and interleukin 1β were also decreased by DE treatment. Moreover, the protein expression of pro inflammatory enzymes, inducible nitric oxide synthase and cyclooxygenase 2 were also dramatically attenuated by DE in a dose dependent manner.ConclusionsThese results suggest that Dictamnus dasycarpus leaf has a potent anti-inflammatory activity and can be used for the development of new anti-inflammatory agents.

Inhibitory effects of indole α‐lipoic acid derivatives on nitric oxide production in LPS/IFNγ activated RAW 264.7 macrophages

“…RAW 264.7 macrophage‐like cells produce nitric oxide as a defence mechanism after activation with LPS and IFNγ. This activation model is widely used to examine and identify compounds as anti‐inflammatory drug candidates . In this study, the effects of three series of indole α ‐lipoic acid derivatives (2.73–87.48 μM) on nitric oxide production and cell viability in activated macrophages were examined.…”

“…This activation model is widely used to examine and identify compounds as anti-inflammatory drug candidates. [25][26][27] In this study, the effects of three series of indole α-lipoic acid derivatives (2.73-87.48 μM) on nitric oxide production and cell viability in activated macrophages were examined. It was also examined whether the inhibitory effect of these three series of indole α-lipoic acid derivatives on nitric oxide production was due to the suppression of iNOS protein expression.…”

Fabrication and Characterization of Human Serum Albumin Particles Loaded with Non-Sericin Extract Obtained from Silk Cocoon as a Carrier System for Hydrophobic Substances

“…Cell metabolic activity was performed on the macrophage cells using MTT assay. The particles 100, 1000, and 5000 particle/cell were chosen for stimulation of RAW 264.7 macrophage cells using nitric oxide (NO) assay [ 21 ]. In brief, macrophage cells were cultured in DMEM medium supplemented with 10% FBS at 37 °C in a 5% CO 2 atmosphere.…”

Anti-inflammatory effects of a methanol extract of Dictamnus dasycarpus Turcz. root bark on imiquimod-induced psoriasis

“…The root bark of this plant contains many bio-active components, such as prenylated flavanone, limonoids, furoquinoline alkaloids, quinoline alkaloids, pyrrolidine alkaloids, sesquiterpenes, coumarins, and phenolic glycosides [12–19]. Previous studies have shown that extracts of this plant have anti-allergenic [20] and anti-inflammatory effects, including reduction of immune cell activity [21], proliferation [10], and recruitment [22]. However, the anti-inflammatory effects of this plant on psoriasis have not previously been studied.…”