Anti-inflammatory activity of the extract, fractions and amides from the leaves of Piper ovatum Vahl (Piperaceae)

Silva, D. Rodrigues; Baroni, Silmara; Svidzinski, Arthur Estivalet; Bersani-Amado, Ciomar Aparecida; Cortez, Diógenes Aparı́cio Garcia

doi:10.1016/j.jep.2007.12.018

Cited by 41 publications

(17 citation statements)

References 20 publications

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“…Fractions 15-32 (23mg) and 42-50 (38mg) were identified as piperovatine (1) and piperlonguminine (2) respectively, by analyses of spectral data of 1 H, 13 C NMR, mass spectrometry and by comparison of data from the literature. [723]…”

Section: Methodsmentioning

confidence: 99%

“…[723] NMR spectra were recorded on a Bruker DRX-400 spectrometer at 300MHz (1H, COSY) and 75.5MHz (13C, DEPT) using chlroform deuterated solvent (CDCl3). They were obtained with TMS as the internal standard and constant temperature of 298 K. In addition, a mass spectrum was obtained by a (EI) Shimadzu gas chromatography coupled with mass spectrometry GC/MS 17A QP 5000 mass spectrometer equipped with a DB5 column (30 m; 0.32mm).…”

Section: Methodsmentioning

confidence: 99%

“…[3] Hydroalcoholic extracts of leaves, piperovatine and piperlonguminine from P. ovatum showed the greatest inhibitory activity of topical inflammation induced by croton oil. [7] The amides piperovatine and piperlonguminine have been isolated from other species of Piper . [891011121314] Recent phytochemical investigations have traced the piscicidal, oral ‘local anesthetic’ and saliva-producing (sialogogic) properties of this species to the isobutyl-amide, piperovatine.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of amide compounds in different parts of Piper ovatum Vahl by high-performance liquid chromatographic

et al. 2013

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Background:Piper ovatum (Piperaceae) has been used in traditional medicine for the treatment of inflammations and as an analgesic. Previous studies have showed important biological activities of the extracts and amides from P. ovatum leaves.Objective:In this study, a high-performance liquid chromatographic (HPLC) method was developed and validated for quantitative determination of the amides in different parts of Piper ovatum.Materials and Methods:The analysis was carried out on a Metasil ODS column (150 × 4.6 mm, 5μm) at room temperature. HPLC conditions were as follows: acetonitrile (A), and water (B), 1.0% acetic acid. The gradient elution used was 0–30 min, 0-60% A; 30–40 min, 60% A. Flow rate used was 1.0mL/min, and detection at 280nm.Results:The validation using piperlonguminine, as the standard, demonstrated that the method shows linearity (linear correlation coefficient = 0.998), precision (relative standard deviation <5%) and accuracy (mean recovery = 103.78%) in the concentration range 31.25 – 500μg/mL. The limit of detection and quantification were 1.21 and 4.03μg/mL, respectively. This method allowed the identification and quantification of piperlonguminine and piperovatine in the hydroethanolic extracts of P. ovatum obtained from the leaves, stems and roots. All the extracts showed the same chromatographic profile. The leaves and roots contained the highest concentrations of piperlonguminine and the stems and leaves showed the most concentrations of piperovatine.Conclusion:This HPLC method is suitable for routine quantitative analysis of amides in extracts of Piper ovatum and phytopharmaceuticals containing this herb.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of amide compounds in different parts of Piper ovatum Vahl by high-performance liquid chromatographic

et al. 2013

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“…Fractionation of this fraction led to purification of the compounds 1 and 2, which were identified by analysis of their spectroscopic data and comparison with literature (McFerren andRodriguez 1998, Wu et al 2004). By analysis of spectral data of the C 13 signal, it was determined that piperlonguminine was the majority substance in the mixture (2:3) (Rodrigues-Silva et al 2008). Figure 1 shows the chromatographic profile of the crude extract.…”

Section: Structure Elucidationmentioning

confidence: 99%

“…Piper ovatum Vahl, an herbaceous plant found throughout Brazil, is popularly known as "joão burandi" or "anestésica," and is used in traditional medicine for the treatment of inflammation and as an analgesic (Correa 1984). The hydroalcoholic extract, fractions, and a mixture of piperovatine and piperlonguminine in a proportion of 2:3 obtained from P. ovatum were assayed for anti-inflammatory activity by means of carrageenan-induced pleurisy in rats and croton oil-induced ear edema in mice (Rodrigues Silva et al 2008). Piperovatine isolated from Ottonia martina Miq.…”

Section: Introductionmentioning

confidence: 99%

In vitro antileishmanial activity of hydroalcoholic extract, fractions, and compounds isolated from leaves of Piper ovatum Vahl against Leishmania amazonensis

Silva¹,

Nakamura²,

Filho³

et al. 2009

Planta Med

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We assessed the biological activity of a crude extract, a mixture of several fractions, and a pure compound obtained from Piper ovatum Vahl against promastigote and amastigote forms of Leishmania amazonensis. The medicinal plant P. ovatum is used popularly as an anesthetic and anti-inflammatory. This study included the extraction process and bioassay-guided fractionation by the adsorption chromatography and Sephadex LH-20 method. A progressive increase in the antileishmanial effect was observed in the course of fractionation. The 50% inhibitory concentration (IC 50) for dichloromethane-ethyl acetate (1:1 v/v) fraction was 2.1 µg/ml and 24 µg/ml; mixture of piperovatine: piperlongumune (2:3) 0.9 µg/ml and 24 µg/ml; piperovatine (1) 9.5 µg/ml and 10 µg/ml; and piperlonguminine (2) 2.5 µg/ml and 9.0 µg/ml, for promastigote and amastigote forms, respectively. Cytotoxicity analysis indicated that these toxic concentrations were much higher for J774G8 macrophages and Vero cells than for the protozoans. The mixture of piperovatine: piperlongumune (2:3) showed important antiprotozoal activity against the amastigote and promastigote forms of L. amazonensis, and it produced morphological changes in promastigotes and amastigotes at 0.9 µg/ml and 24 µg/ml (50% growth inhibition concentration), respectively, including intense cytoplasmic vacuolization, mitochondrial swelling, and mitochondrial damage, as revealed by transmission electron microscopy.

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Anticholinesterase, Antidiabetic and Anti-inflammatory Activity of Secondary Metabolites of Teucrium Species

Zlatić

Stanković

2020

Teucrium Species: Biology and Applications

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Anti-inflammatory activity of the extract, fractions and amides from the leaves of Piper ovatum Vahl (Piperaceae)

Cited by 41 publications

References 20 publications

Analysis of amide compounds in different parts of Piper ovatum Vahl by high-performance liquid chromatographic

Analysis of amide compounds in different parts of Piper ovatum Vahl by high-performance liquid chromatographic

In vitro antileishmanial activity of hydroalcoholic extract, fractions, and compounds isolated from leaves of Piper ovatum Vahl against Leishmania amazonensis

Anticholinesterase, Antidiabetic and Anti-inflammatory Activity of Secondary Metabolites of Teucrium Species

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