Anti-inflammatory and anti-nociceptive effect of Betula platyphylla var. japonica in human interleukin-1β-stimulated fibroblast-like synoviocytes and in experimental animal models

Huh, Jungwon; Hong, Jang-Mu; Baek, Yong-Hyeon; Lee, Jae-Dong; Choi, Dong‐Hee; Park, Dong-Suk

doi:10.1016/j.jep.2011.03.005

Cited by 30 publications

(24 citation statements)

References 36 publications

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“…MMPs and PGE 2 could directly degrade collagen and proteoglycan in cartilage and bone matrix, respectively. The proinflammatory cytokine IL-6 is able to significantly up-regulate both the expressions of MMPs and PGE 2 in chondrocytes and fibroblasts of RA [19] . As Figure 4A shown, the serum levels of IL-6, PGE 2 , MMP-1, and MMP-13 in AIA model rats were all dramatically increased; NOR (15 and 30 mg/kg) and DEX (0.5 mg/kg) treatments markedly decreased IL-6, PGE 2 and MMP-13 levels in serum but had little effect on MMP-1 levels.…”

Section: Resultsmentioning

confidence: 98%

Norisoboldine alleviates joint destruction in rats with adjuvant-induced arthritis by reducing RANKL, IL-6, PGE2, and MMP-13 expression

et al. 2013

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Aim: To explore the effects of norisoboldine (NOR), a major isoquinoline alkaloid in Radix Linderae, on joint destruction in rats with adjuvant-induced arthritis (AIA) and its underlying mechanisms. Methods: AIA was induced in adult male SD rats by intradermal injection of Mycobacterium butyricum in Freund's complete adjuvant at the base of the right hind paw and tail. From d 14 after immunization, the rats were orally given NOR (7.5, 15, or 30 mg/kg) or dexamethasone (0.5 mg/kg) daily for 10 consecutive days. Joint destruction was evaluated with radiological scanning and H&E staining. Fibroblast-like synoviocytes (FLS) were prepared from fresh synovial tissues in the AIA rats. The expression of related proteins and mRNAs were detected by ELISA, Western blotting and RT-PCR. Results: In AIA rats, NOR (15 and 30 mg/kg) significantly decreased the swelling of paws and arthritis index scores, and elevated the mean body weight. NOR (30 mg/kg) prevented both the infiltration of inflammatory cells and destruction of bone and cartilage in joints. However, NOR (15 mg/kg) only suppressed the destruction of bone and cartilage, but did not obviously ameliorate synovial inflammation. NOR (15 and 30 mg/kg) significantly decreased the serum levels of receptor activator of nuclear factor κB ligand (RANKL), IL-6, PGE 2 , and MMP-13, but not the osteoprotegerin and MMP-1 levels. The mRNA levels of RANKL, IL-6, COX-2, and MMP-13 in synovium were also suppressed. Dexamethasone produced similar effects in AIA rats as NOR did, but without elevating the mean body weight. In the cultured FLS, treatment with NOR (10 and 30 mmol/L) significantly decreased the secretion of RANKL, IL-6, PGE 2 , and MMP-13 proteins. Furthermore, the treatment selectively prevented the activation of MAPKs, AKT and transcription factor AP-1 component c-Jun, but not the recruitment of TRAF6 or the activation of JAK2/STAT3. Treatment of the cultured FLS with the specific inhibitors of p38, ERK, AKT, and AP-1 significantly decreased the secretion of RANKL, IL-6, PGE 2 , and MMP-13 proteins. Conclusion: NOR can alleviate joint destruction in AIA rats by reducing RANKL, IL-6, PGE 2 , and MMP-13 expression via the p38/ERK/ AKT/AP-1 pathway.

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Section: Resultsmentioning

confidence: 98%

Norisoboldine alleviates joint destruction in rats with adjuvant-induced arthritis by reducing RANKL, IL-6, PGE2, and MMP-13 expression

et al. 2013

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“…The regulation of undesired immune response is necessary for the prevention of many severe diseases development from acute to chronic stage. Non-steroidal anti-inflammatory drugs are clinically common as an inhibitor of NF-kB-dependent inflammatory genes of TNF-a and IL-1b (Lee & Burckart, 1998); but these drugs carry the risk of gastrointestinal toxicity thus, their use is limited (Huh et al, 2011). Nowadays, the discovery of many pharmacological new drugs contains a natural products or natural product analogs.…”

Section: Discussionmentioning

confidence: 99%

Bioassay guided fractionation and identification of active anti-inflammatory constituent fromDelonix elataflowers using RAW 264.7 cells

Saravanan

Ibrahim

David³

et al. 2014

Pharmaceutical Biology

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Context: Delonix elata (L.) Gamble (Fabaceae) has been used in the Indian traditional medicine system to treat rheumatism and inflammation. Aim: To assess the anti-inflammatory effect of Delonix elata flowers and to isolate the active principle. Materials and methods: The prompt anti-inflammatory constituent was isolated from Delonix elata flower extracts using bioassay guided fractionation in liposaccharide (LPS) stimulated RAW 264.7 macrophage cell line. The anti-inflammatory activity of extracts/fractions/subfractions/compounds (10, 25, and 50 mg/ml) was evaluated by estimating the levels of nitric oxide (NO), TNF-a, and IL-1b after 24 h of LPS induction (1 mg/ml). The isolated active compound was subjected to NMR, IR, and UV analyses for structure determination. Results: In an attempt to search for anti-inflammatory constituents, the active pure principle was isolated and crystallized as a white compound from Delonix elata flowers methanol extract. This active compound (50 mg/ml) decreased the release of inflammatory mediators levels such as NO (0.263 ± 0.03 mM), TNFa (160.20 ± 17.57 pg/ml), and IL-1b (285.79 ± 15.16 pg/ml) significantly (p50.05); when compared to the levels of NO (0.774 ± 0.08 mM), TNFa (501.71 ± 25.14 pg/ml), and IL-1b (712.68 ± 52.25 pg/ml) from LPS-stimulated macrophage cells. The active compound was confirmed as hesperidin with NMR, IR, and UV spectroscopy data. This is the first report of this compound from Delonix elata flowers. Conclusion: The findings of the study support the traditional use of Delonix elata flowers to treat inflammation.

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“…2) (Freysdottir et al 2011;Grundemann et al 2011;Huh et al 2011). A number of new biologically active compounds have recently been isolated, especially from Asiatic birch (Phan et al 2011;Xiong et al 2011).…”

Section: Birch (Betula L)mentioning

confidence: 99%

Hybridisation, Introgression and Phylogeography of Icelandic Birch

Anamthawat‐Jónsson¹

2012

Current Topics in Phylogenetics and Phylogeography of Terrestrial and Aquatic Systems

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Anti-inflammatory and anti-nociceptive effect of Betula platyphylla var. japonica in human interleukin-1β-stimulated fibroblast-like synoviocytes and in experimental animal models

Cited by 30 publications

References 36 publications

Norisoboldine alleviates joint destruction in rats with adjuvant-induced arthritis by reducing RANKL, IL-6, PGE2, and MMP-13 expression

Norisoboldine alleviates joint destruction in rats with adjuvant-induced arthritis by reducing RANKL, IL-6, PGE2, and MMP-13 expression

Bioassay guided fractionation and identification of active anti-inflammatory constituent fromDelonix elataflowers using RAW 264.7 cells

Hybridisation, Introgression and Phylogeography of Icelandic Birch

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