Anti-inflammatory and Antioxidant Activities of the Extracts from Leaves and Stems of Polygonum odoratum Lour

Abstract

Background: Polygonum odoratum is an indigenous vegetable that has been used as a favoring agent and also used as a Thai traditional medicine to treat flatulence. </P><P> Objective: To analyze active ingredients, total phenolic and total flavonoid contents, antiinflammatory and antioxidant activities from leaf and stem extracts of P. odoratum. </P><P> Methods: Leaves and stems were dried and extracted by using methanol, dichloromethane and water for obtaining Methanolic Leaf Extract (MLE), Methanolic Stem Extract (MTE), Dichloromethane Leaf Extract (DLE), Dichloromethane Stem Extract (DTE), Water Leaf Extract (WLE) and Water Stem Extract (WTE). The extracts were quantified for total phenolic and total flavonoid contents by spectrophotometry and active compounds were analyzed by using GC-MS. Antioxidant activity was determined by ABTS and DPPH radicals scavenging assays. Anti-inflammatory activity was tested by the inhibition of nitric oxide production in RAW 264.7 macrophage cells induced by lipopolysaccharide. </P><P> Results: The DLE exhibited the most potent anti-inflammatory effect by inhibiting nitric oxide production in a concentration-dependent manner (IC50 = 53.75±0.72 µg/mL). MLE exhibited strong antioxidant activity and contained the highest concentration of phenolic compounds (52.59±0.58 mg gallic acid equivalent/g extract) and flavonoid (19.97+0.11 mg quercetin equivalent/g extract). E-15-Heptadecenal and 3, 7, 11, 15-tetramethyl-2- hexadecen-1-ol were found predominantly in the methanol extracts. </P><P> Conclusion: The leaf extract of P. odoratum showed potent anti-inflammatory and antioxidant activities, mediated by DLE and MLE, respectively.

Optimization of the Extraction Conditions and Biological Evaluation of Dendropanax morbifera H. Lev as an Anti-Hyperuricemic Source

“…According to previous reports, the antioxidant effects of plant extracts have curative benefits against conditions such as inflammation, oxidative stress, and other metabolic diseases such as hyperuricemia and gout arthritis [10,14,15]. Thus, we compared the antioxidant effects of the hexane and ethanol extracts.…”

Polygonum odoratum leaf extract attenuates oxidative stress and cell death of Raw 264.7 cells exposed to low dose ionizing radiation

“…Since POE provided a high phenolic compound and contained numerous active substances which are presented in the form of phenol OH, POE exerted a high antioxidant activity in both DPPH radical scavenging assay and FRAP assay. leaves has no cytotoxicity to RAW 264.7 cells with cell viability more than 70% at the concentration of 200 µg/ml (Chansiw et al, 2019).…”

“…Although P. odoratum leaves have been widely consumed as a vegetable, the evaluation of cytotoxicity to the tested cell was also important. The maceration extraction with water of P. odoratum leaves has no cytotoxicity to RAW 264.7 cells with cell viability more than 70% at the concentration of 200 µg/ml (Chansiw et al., 2019). Interestingly, POE provided the same trend of cell viability, but up to 1,250 µg/ml which is higher than reported in the previous study.…”

“…Polygonum odoratum Lour or Pak Paew is a traditional herb that has been widely consumed in Thailand. It consists of many bioactive compounds such as phenolic acids and flavonoids that can exhibit potent antioxidant and anti-inflammatory activities (Ahongshangbam et al, 2014;Chansiw et al, 2019). In addition, it has been demonstrated that the functional food containing combined extracts between Morus alba and P. odoratum leaf could decrease the oxidative damage and exhibit antiosteoporotic effects (Sungkamanee et al, 2014).…”

Optimization of total flavonoid content of ethanolic extract of Persicaria pulchra (Bl.) Soják for the inhibition of α-glucosidase enzyme

“…TPC and TFC of the plant are 388 mg GA/g and 423 mg QUE/g, respectively. Interestingly, TPC and TFC of P. pulchra were much higher (35) 0 (70) 0 (10) 0 (65) 437 ± 11 2 (75) 0 (70) 0 (10) 0 (65) 440 ± 9 0 (55) −2 (10) 0 (10) 0 (65) 449 ± 4 0 (55) 2 (130) 0 (10) 0 (65) 426 ± 14 0 (55) 0 (70) −2 (6) 0 (65) 504 ± 10 0 (55) 0 (70) 2 (14) 0 (65) 474 ± 14 0 (55) 0 (70) 0 (10) −2 (35) 461 ± 12 0 (55) 0 (70) 0 (10) 2 (95) 456 ± 13 0 (55) 0 (70) 0 (10) 0 (65) 534 ± 10 0 (55) 0 (70) 0 (10) 0 (65) 529 ± 9 0 (55) 0 (70) 0 (10) 0 (65) 536 ± 12 0 (55) 0 (70) 0 (10) 0 (65) 521 ± 14 0 (55) 0 (70) 0 (10) 0 (65) 529 ± 7 0 (55) 0 (70) 0 (10) 0 (65) 521 ± 12 0 (55) 0 (70) 0 (10) 0 (65) 528 ± 8 0 (55) 0 (70) 0 (10) 0 (65) 530 ± 7 0 (55) 0 (70) 0 (10) 0 (65) 532 ± 15 0 (55) 0 (70) 0 (10) 0 (65) 536 ± 7 0 (55) 0 (70) 0 (10) 0 (65) 522 ± 10 0 (55) 0 (70) 0 (10) 0 (65) 529 ± 9 than those of other plants in the Persicaria family, such as P. odoratum and P. minus [28,29]. The crude ethanolic extract of P. pulchra exhibited good inhibition of α-glucosidase with IC 50 of 33.53 ± 1.25 µg/mL, which is comparable to those of some anti-diabetic Vietnamese plants, like N. mirabilis, P. urinaria, and K. candel [30].…”