Anti‐inflammatory and antiradical effects of a 2% diosmin cream in a human skin organ culture as model

Boisnic, S; Branchet, Marie‐Christine; Gouhier-Kodas, Christelle; Verrière, François; Jabbour, Violaine

doi:10.1111/jocd.12778

Cited by 18 publications

(11 citation statements)

References 14 publications

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“…Although pharmacotherapy combined with compression and sometimes surgical intervention is the most effective approach in CVD treatment, topical treatment may alleviate some symptoms and prevent unfavorable processes in skin. Diosmin is commercially available in the form of gel, ointment, or emulsion for topical application; however, scientific reports on its efficacy are scarce [9]. Therefore, the aim of the present paper was to investigate the anti-inflammatory activity of diosmin and its aglycone diosmetin in a human skin fibroblast cell culture treated with bacterial lipopolysaccharide (LPS).…”

Section: Introductionmentioning

confidence: 99%

Modulatory Effect of Diosmin and Diosmetin on Metalloproteinase Activity and Inflammatory Mediators in Human Skin Fibroblasts Treated with Lipopolysaccharide

et al. 2022

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Diosmin is widely used as a venoactive drug in the pharmacological treatment of chronic venous disorders. It exerts a strong protective effect on blood vessels via an increase in the elasticity of vessel walls and reduces the permeability of capillary walls, thereby producing an anti-edematous effect. In this paper, we investigated the effectiveness of diosmin and diosmetin in modulating the level of proinflammatory factors in human skin fibroblasts treated with lipopolysaccharide (LPS). Two variants of the experiments were performed: the flavonoid was added 2 h prior to or 24 h after LPS stimulation. Our study revealed that both flavonoids reduced the levels of IL-6 and Il-1β as well as COX-2 and PGE2 but had no impact on IL-10. However, the addition of the compounds prior to the LPS addition was more effective. Moreover, diosmetin modulated the proinflammatory factors more strongly than diosmin. Our investigations also showed that both flavonoids were potent inhibitors of elastase and collagenase activity, and no differences between the glycoside and aglycone forms were observed.

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Section: Introductionmentioning

confidence: 99%

Modulatory Effect of Diosmin and Diosmetin on Metalloproteinase Activity and Inflammatory Mediators in Human Skin Fibroblasts Treated with Lipopolysaccharide

et al. 2022

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“…The biological mechanisms underlying the anti-inflammatory beneficiary effects of diosmin are well documented, which consistently include inhibition of inflammatory reactions through a potent reduction in prostaglandin E2 and thromboxane A2 and inhibition of leukocyte and adhesion. 70,77 It's interesting to note diosmin's anti-inflammatory effect precedes its antioxidant effect after 7 days of treatment. Our data suggest that diosmin is potently anti-inflammatory, and after some delay activates eNOS to induce further anti-inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…They determined that animals pre‐treated with diosmin exhibited less neutrophil adhesion in the post‐capillary venules compared to the control group. The biological mechanisms underlying the anti‐inflammatory beneficiary effects of diosmin are well documented, which consistently include inhibition of inflammatory reactions through a potent reduction in prostaglandin E2 and thromboxane A2 synthesis and inhibition of leukocyte activation/migration and adhesion 70,77 . It's interesting to note that diosmin's anti‐inflammatory effect precedes its antioxidant effect after 7 days of treatment.…”

Section: Discussionmentioning

confidence: 99%

Diosmin and its glycocalyx restorative and anti‐inflammatory effects on injured blood vessels

et al. 2022

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The endothelium, a crucial homeostatic organ, regulates vascular permeability and tone. Under physiological conditions, endothelial stimulation induces vasodilator endothelial nitric oxide (eNO) release and prevents adhesion molecule accessibility and leukocyte adhesion and migration into vessel walls. Endothelium dysfunction is a principal event in cardiovascular disorders, including atherosclerosis. Minimal attention is given to an important endothelial cell structure, the endothelial glycocalyx (GCX), a negatively charged heterogeneous polysaccharide that serves as a protective covering for endothelial cells and enables endothelial cells to transduce mechanical stimuli into various biological and chemical activities. Endothelial GCX shedding thus plays a role in endothelial dysfunction, for example by increasing vascular permeability and decreasing vessel tone.Consequently, there is increasing interest in developing therapies that focus on GCX repair to limit downstream endothelium dysfunction and prevent further downstream cardiovascular events. Here, we present diosmin (3′,5,7-trihydroxy-4′-methoxyflavone-7-rhamnoglucoside), a flavone glycoside of diosmetin, which downregulates adhesive molecule expression, decreases inflammation and capillary permeability, and upregulates eNO expression. Due to these pleiotropic effects of diosmin on the vasculature, a possible unidentified mechanism of action is through GCX restoration. We hypothesize that diosmin positively affects GCX integrity along with GCX-related endothelial functions. Our hypothesis was tested in a partial ligation left carotid artery (LCA) mouse model, where the right carotid artery was the control for each mouse. Diosmin (50 mg/kg) was administered daily for 7 days, 72 h after ligation. Within the ligated mice LCAs, diosmin How to cite this article: Mitra R, Nersesyan A, Pentland K, Melin MM, Levy RM, Ebong EE. Diosmin and its glycocalyx restorative and anti-inflammatory effects on injured blood vessels.

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“…The endothelial cell basal medium (ECBM, PromoCell) with bovine serum albumin and growth factors was added to the bottom of the wells, allowing diffusion between the two compartments separated by the porous membrane. The skin explants remained viable ex vivo for 4 days, corresponding to the time during which controls remained untreated, and test explants were in contact with the study product 14 …”

Section: Methodsmentioning

confidence: 99%

Purple tulip extract improves signs of skin aging through dermal structural modulation as shown by genomic, protein expression and skin appearance of volunteers studied

Hajem¹,

Manzato²,

Branchet³

et al. 2020

J of Cosmetic Dermatology

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Background Purple tulip extract is a rich source of flavonoids which are powerful antioxidants and can hence be considered as an ideal candidate for use in skin care products. Aims We aimed to evaluate the effects of purple tulip extract on skin quality and to determine its molecular modes of interaction. Methods A pangenomic study on human skin fibroblasts was carried out to analyze multiple changes in gene expression. Ex vivo studies of human skin explants exposed to ultraviolet (UV) irradiation or H2O2 were performed to assess modulations of protein expression. Finally, a clinical assay was carried out to evaluate the efficacy of purple tulip extract on skin appearance and condition of aged women. Results Genetic modulation analyses led us to infer the induction of many biological functions including cell differentiation, proliferation, migration, inflammatory responses, and matrix remodeling. The ex vivo studies revealed an enhancement of the collagen network and increased expression of glycosaminoglycans (GAG), fibronectin, and collagen VI. Finally, the clinical study highlighted the potential anti‐aging properties of the purple tulip extract which decreased the relaxation of the oval face and improved skin elasticity after 28 days of treatment. Significant reductions of the length and depth of the nasolabial wrinkles were also observed. Conclusion Our genomics data on the effect of purple tulip extract on the ex vivo UV‐challenged skin showed that genes responsible for, among others, the upkeep of the skin, such as collagen induction, immune cell proliferation, and epidermal repair, were all up‐regulated. More importantly, the clinical study corroborated these data by the visible and measurable effects of the topical purple tulip extract on the aged skin of 22 women, further demonstrating the beneficial impact of the extract on aged skin.

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Anti‐inflammatory and antiradical effects of a 2% diosmin cream in a human skin organ culture as model

Abstract: Diosmin administered topically may protect skin against the biological effects of various exogenous or endogenous stresses, such as those involved in chronic venous disease.

Cited by 18 publications

References 14 publications

Modulatory Effect of Diosmin and Diosmetin on Metalloproteinase Activity and Inflammatory Mediators in Human Skin Fibroblasts Treated with Lipopolysaccharide

Modulatory Effect of Diosmin and Diosmetin on Metalloproteinase Activity and Inflammatory Mediators in Human Skin Fibroblasts Treated with Lipopolysaccharide

Diosmin and its glycocalyx restorative and anti‐inflammatory effects on injured blood vessels

Purple tulip extract improves signs of skin aging through dermal structural modulation as shown by genomic, protein expression and skin appearance of volunteers studied

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