Anti-inflammatory and antispasmodic activity of Ipomoea imperati (Vahl) Griseb (Convolvulaceae)

Abstract

Ipomoea imperati (Convolvulaceae) lives on the sandy shores of the Brazilian coast and in other areas of the world. The anti-inflammatory activity of a methanol-water extract of the leaves of I. imperati was investigated in experimental models of acute and subchronic inflammation. Topical application of the extract (10 mg/ear) inhibited mouse ear edema induced by croton oil (89.0 ± 1.3% by the lipid fraction with an IC 50 of 3.97 mg/ear and 57.0 ± 1.3% by the aqueous fraction with an IC 50 of 3.5 mg/ear) and arachidonic acid (42.0 ± 2.0% with an IC 50 of 4.98 mg/ear and 31.0 ± 2.0% with an IC 50 of 4.72 mg/ear). Phospholipase A 2 , purified from Apis mellifera bee venom, was also inhibited by the extract (5.0 mg/ml lipid and aqueous fraction) in vitro in a dose-dependent manner (85% by the lipid fraction with an IC 50 of 3.22 mg/ml and 25% by the aqueous fraction with an IC 50 of 3.43 mg/ ml). The methanol-water extract of I. imperati (1000 mg/kg) administered by the oral route also inhibited the formation of cotton pelletinduced granulomas (73.2 ± 1.2% by the lipid fraction and 56.14 ± 2.7% by the aqueous fraction) and did not cause gastric mucosal lesions. I. imperati extracts (10 mg/ml) also inhibited in a dosedependent manner the muscle contractions of guinea pig ileum induced by acetylcholine and histamine (IC 50 of 1.60 mg/ml for the lipid fraction and 4.12 mg/ml for the aqueous fraction). These results suggest the use of I. imperati as an anti-inflammatory and antispasmodic agent in traditional medicine. Correspondence

Review of the genus Ipomoea: traditional uses, chemistry and biological activities

“…This extract also presented antispasmodic activity on the isolated ileum, inhibiting histamine and acetylcholine. In the acute toxicity assay, 1 mg/kg of I. imperati methanol-water extract caused no mortality in mice after 24 h (Paula et al, 2003). Ipomoea imperati prevented the formation of gastric lesions in 78% (p<0.05) when compared with the negative control tween 80 (Miyahara et al, 2011).…”

“…stolonifera As diuretic and to treat pain after childbirth, stomach problems, inflamations, furunculosis, swelling and wound (Paula et al, 2003). 65 R 1 =H; R 2 =α-methyl-butanoyl; R 3 =2-methyl-3-hydroxy-butanoyl; R 4 =tigloyl 66 R 1 =H; R 2 =isobutanoyl; R 3 =2-methyl-3-hydroxy-butanoyl; R 4 =tigloyl 67 R 1 =H; R 2 =R 3 =2-methyl-3-hydroxy-butanoyl; R 4 =tigloyl 68 R 1 =isobutanoyl; R 2 =H; R 3 =2-methyl-3-hydroxy-butanoyl; R 4 =tigloyl Indolizidine alkaloids were isolated from the seeds of I. alba: ipalbine ipalbidine, isoipomine, ipalbidinium, E-ipomine, Z-ipomine, methoxyipomine, dimethoxyipomine and ipohardine (Ikhiri et al, 1987;Gourley et al, 1969).…”

“…This extract also presented antispasmodic activity on the isolated ileum, inhibiting histamine and acetylcholine. In the acute toxicity assay, 1 mg/kg of I. imperati methanol-water extract caused no mortality in mice after 24 h (Paula et al, 2003). From the ether-soluble resin glycoside fraction was isolated twelve glycoresins called stoloniferins I-XII were isolated in the pure state from the whole plants of I. stolonifera, but its biological activities were not reported (Noda et al, 1994;.…”

Appraisal of ethnobotanical uses of the wetland plants of Punjab, Pakistan

“…Therefore, nowadays various folk medicines in single, or in combination are used to treat diverse types of inflammatory and arthritic ailments (Paula et al, 2003). Medicines derived from raw materials of plants are also getting popularity due to the belief that "Green medicine" is secure with fewer side effects than the synthetic drugs (Parekh & Chanda, 2007).…”

Antinociceptive activity of Ipomoea imperati (Vahl) Griseb., Convolvulaceae

“…I. imperati had a significant inhibitory activity against phospolipase A2 enzyme from bee venom (Paula et al, 2003). Souza et al (2000) related that other species of the Convolvulaceae family, such as Ipomoea pes-caprae, has showed an antinociceptive effect against two classical models of pain in mice.…”