Anti‐inflammatory and immunomodulatory activities of polysaccharide from <i>Chlorella stigmatophora</i> and <i>Phaeodactylum tricornutum</i>

Guzman, Stacy; Gato, A.; Lamela, M.; Freire‐Garabal, Manuel; Calleja, José María

doi:10.1002/ptr.1227

Cited by 239 publications

(98 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These results are evidenced that the increased cell viability of the samples against LPS treated toxicity followed by significant suppression of NO production as well. Recently reported many publications have indicated that containing of anti-inflammatory components in P. tricornutum and Chlorella stigmatophora organic solvent extracts (Guzmán et al 2003). Therefore, these results were suggested that the strong anti-inflammatory activities were observed in the extracts and the fractions derived from the cultured marine microalgae in vitro assay.…”

Section: Anti-inflammatory Effect Of the Extracts And Fractions From mentioning

confidence: 83%

In vitro studies of anti-inflammatory and anticancer activities of organic solvent extracts from cultured marine microalgae

Samarakoon¹,

Ko²,

Shah³

et al. 2013

ALGAE

View full text Add to dashboard Cite

Marine microalgae are a promising source of organisms that can be cultured and targeted to isolate the broad spectrum of functional metabolites. In this study, two species of cyanobacteria, Chlorella ovalis Butcher and Nannchloropsis oculata Droop, one species of bacillariophyta, Phaeoductylum tricornutum Bohlin, and one species of Dinophyceae, Amphidinium carterae (Hulburt) were cultured and biomasses used to evaluate the proximate comical compositions. Among the determined proximate chemical compositions of the cultured marine microalgae, the highest content of crude proteins and lipids were exhibited in P. tricornutum and A. carterae, respectively. Solvent-solvent partition chromatography was subjected to fractionate each of the cultured species and separated n-hexane, chloroform, ethyl acetate, and aqueous fractions. Nitric oxide production inhibitory level (%) and cytotoxicity effect on lipo-polysaccharide-induced RAW 264.7 macrophages were performed to determine the anti-inflammatory activity. N. oculata hexane and chloroform fractions showed significantly the strongest anti-inflammatory activity at 6.25 µg mL -1 concentration. The cancer cell growth inhibition (%) was determined on three different cell lines including HL-60 (a human promyelocytic leukemia cell line), A549 (a human lung carcinoma cell line), and B16F10 (a mouse melanoma cell line), respectively. Among the extracts, C. ovalis ethyl acetate and A. carterae chloroform fractions suppressed the growth of HL-60 cells significantly at 25 and 50 µg mL -1 concentrations. Thus, the cultured marine microalgae solvent extracts may have potentiality to isolate pharmacologically active metabolites further using advance chromatographic steps. Hence, the cultured marine microalgae can be described as a good candidate for the future therapeutic uses.

show abstract

Section: Anti-inflammatory Effect Of the Extracts And Fractions From mentioning

confidence: 83%

In vitro studies of anti-inflammatory and anticancer activities of organic solvent extracts from cultured marine microalgae

Samarakoon¹,

Ko²,

Shah³

et al. 2013

ALGAE

View full text Add to dashboard Cite

show abstract

“…[32][33][34][35] Few in vitro and in vivo studies on Chlorella extracts have shown anti-inflammatory, immunomodulatory, analgesic, and free radical scavenging activities. 36,37 Authors have reported that Chlorella short-term supplementation in uninfected normal people enhanced Natural Killer cell activity and early inflammatory response (serum concentration of interferon-c and interleukin-1b increased). 38 It is difficult to determine which compounds could be responsible for such immunomodulatory or analgesic effects as the microalgae Chlorella is a mix of many components as various as proteins, lipids, carbohydrates, pigments, and also not so well-characterized compounds yet such as growth factors, the whole probably having a synergistic effect.…”

Section: Discussionmentioning

confidence: 99%

Benefits of Preventive Administration ofChlorellasp. on Visceral Pain and Cystitis Induced by a Single Administration of Cyclophosphamide in Female Wistar Rat

Hidalgo-Lucas¹,

Rozan²,

Guérin‐Deremaux

et al. 2016

Journal of Medicinal Food

View full text Add to dashboard Cite

Chlorella sp. is a green microalgae containing nutrients, vitamins, minerals, and chlorophyll. In some communities, Chlorella sp. is a traditional medicinal plant used for the management of inflammation-related diseases. In a rat model, ROQUETTE Chlorella sp. (RCs) benefits were investigated on visceral pain and associated inflammatory parameters related to cystitis both induced by cyclophosphamide (CYP). RCs was orally administered every day from day 1-16 (250 and 500 mg/kg body weight). Six hours after an intraperitoneal injection of 200 mg/kg body weight of CYP, body temperature, general behavior, food intake, and body weight were recorded. Twenty-four hours after CYP injection, rats were tested in two behavioral tests, an open field and the aversive light stimulus avoidance conditioning test, to evaluate the influence of pain on general activity and learning ability of rats. After euthanasia, bladders were weighed, their thickness was scored, and the urinary hemoglobin was measured. RCs orally administered at the two dosages significantly reduced visceral pain and associated inflammatory parameters related to cystitis both induced by CYP injection, and improved rat behavior. To conclude, RCs demonstrated beneficial effects against visceral pain and cystitis.

show abstract

“…The extracts of PT show anti-inflammatory effects in the paw edema test and immunomodulatory effects in the delayed hypersensitivity test (Guzman et al, 2001). A pharmacological study of hydrosoluble and liposoluble extracts of the marine microalgae Chlorella stigmatophora and PT indicated that hydrosoluble components of both species show significant antiinflammatory, analgesic and free radical scavenging activity (Guzman et al, 2003). These activities were not detected in the liposoluble fractions (Guzman et al, 2001;.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Anti-inflammatory Activities and Mechanisms of Microalga Phaeodactylum tricornutum

Kim¹,

Kim²,

Pan³

et al. 2013

Journal of Applied Biological Chemistry

View full text Add to dashboard Cite

Due to their diversity and abundancy, marine resources have emerged as important biological resources to compensate the limited sources of terrestrial biological materials. Phaeodactylum tricornutum (PT) is one of classical model diatoms most widely studied for its ecology, physiology, biochemistry and molecular biology. In this study, four different PT extracts on lipopolysaccharide (LPS)-stimulated macrophages were compared for anti-inflammatory effect and investigated for the underlying mechanisms. The extracts of PT inhibited nitric oxide production from LPS stimulated RAW 264.7 cells in a dose dependent manner. These extracts also inhibited the expression of mRNA and production of proteins of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6 and tumor necrosis factor-α. These inhibitory effects were found to be caused by blockage of nuclear factor-κB activation and phosphorylation of p38 mitogen-activated protein kinases, extracellular signal-regulated kinases 1 and 2 and c-Jun N-terminal kinase.

show abstract

Anti‐inflammatory and immunomodulatory activities of polysaccharide from Chlorella stigmatophora and Phaeodactylum tricornutum

Cited by 239 publications

References 13 publications

In vitro studies of anti-inflammatory and anticancer activities of organic solvent extracts from cultured marine microalgae

In vitro studies of anti-inflammatory and anticancer activities of organic solvent extracts from cultured marine microalgae

Benefits of Preventive Administration ofChlorellasp. on Visceral Pain and Cystitis Induced by a Single Administration of Cyclophosphamide in Female Wistar Rat

Evaluation of Anti-inflammatory Activities and Mechanisms of Microalga Phaeodactylum tricornutum

Contact Info

Product

Resources

About