Anti-inflammatory bibenzyls from the stems of Dendrobium huoshanense via bioassay guided isolation

Abstract

The stems of Dendrobium huoshanense have long been used to prevent various diseases, including inflammatory diseases. This study was aimed to explain the anti-inflammatory effect of D. huoshanense stems in LPS-induced RAW 264.7 macrophages and to discover potential anti-inflammatory compounds. Results exhibited that D. huoshanense stems ethanol extract could significantly inhibit LPS-induced production of NO, TNF-α and IL-1β. Based on bioassay guided strategy, four bibenzyls (1-4) were isolated from D. huoshanense stems for the first time.Anti-inflammatory assay showed 1-4 could remarkably inhibit the production of NO in LPS-induced macrophages. Moreover, quantitative RT-PCR analysis displayed that the mRNA levels of iNOs, TNF-α and IL-1β could also be significantly reduced by 1-4. These results suggested that D. huoshanense stems ethanol extract and bibenzyls 1-4 might be well developed as therapeutic agent to prevent inflammatory diseases.

Structure–antioxidant activity relationships of dendrocandin analogues determined using density functional theory

“…Dendrocandins (DDCDs) comprise a family of compounds originally extracted from the stem of Dendrobium plants [18][19][20][21][22][23]. The structure of DDCD was elucidated as a stilbenolignan skeleton in which a bibenzyl moiety is linked to a phenylpropane unit via a dioxane bridge.…”

“…The structure of DDCD was elucidated as a stilbenolignan skeleton in which a bibenzyl moiety is linked to a phenylpropane unit via a dioxane bridge. DDCDs are highly bioactive, exhibiting antioxidant, anticancer, and anti-inflammatory activities [18][19][20][21][22][23][24]. As a class of important compounds in Dendrobium species, DDCDs have attracted considerable and growing interest among scientists.…”

Detection of Mercury Ion with High Sensitivity and Selectivity Using a DNA/Graphene Oxide Hybrid Immobilized on Glass Slides

“…Recently, researchers are devoted to the development of fast, sensitive, and cost-effective portable biosensors for Hg 2+ , in which the methods generally include electrochemistry [ 16 , 17 ], field-effect transistors [ 18 ], surface plasmon resonance [ 19 , 20 ], Raman spectroscopy [ 21 ], colorimetric detection [ 22 , 23 ], and fluorescence [ 24 ]. Compared with traditional sensors, biochips can not only achieve high-throughput detection but also meet portability and efficiency [ 25 ], which have been developed to detect DNA [ 26 ], proteins/peptides [ 27 ], and heavy metal ions [ 28 ].…”

Structure-antioxidant Activity Relationships of Dendrocandin Analogues Determined Using Density Functional Theory

“…Dendrocandins (DDCDs) comprise a family of compounds originally extracted from the stem of Dendrobium plants [18][19][20][21][22][23]. The structure of DDCD was elucidated as a stilbenolignan skeleton in which a bibenzyl moiety is linked to a phenylpropane unit via a dioxane bridge.…”

“…The structure of DDCD was elucidated as a stilbenolignan skeleton in which a bibenzyl moiety is linked to a phenylpropane unit via a dioxane bridge. DDCDs are highly bioactive, exhibiting antioxidant, anticancer, and anti-in ammatory activities [18][19][20][21][22][23][24]. As a class of important compounds in Dendrobium species, DDCDs have attracted considerable and growing interest among scientists.…”