Anti-inflammatory components of Euphorbia humifusa Willd.

Luyen, Bui Thi Thuy; Tài, Bùi Hữu; Thao, Nguyen Phuong; Eun, Kim Ji; Yun, Ji; Xin, Ming Jie; Lee, Young Mi; Kim, Young Ho

doi:10.1016/j.bmcl.2014.03.014

Cited by 51 publications

(18 citation statements)

References 37 publications

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“…Modern pharmacological studies also revealed various significant bioactivities of the above herbs and their active compounds (quercetin, berberine, matrine, etc. ), including anti-diarrheal, antibacterial, antifungal, antioxidant, anti-inflammatory, antiviral and analgesic activities ( Yi et al, 2013 ; Luyen et al, 2014 ), and they may contribute to the anti-diarrheal activity of DHP. For example, as the main principle of DHP, Coptidis Rhizoma ( Huanglian ) and its active compound berberine with the significant antimicrobial, antiprotozoal, antidiarrheal, and antitrachoma and other activities, have been made to anti-diarrheal agents, and widely used to treat diarrhea and dysentery in China ( Committee for the Pharmacopoeia of P.R.…”

Section: Discussionmentioning

confidence: 99%

The Anti-diarrheal Activity of the Non-toxic Dihuang Powder in Mice

Shang¹,

Miao²,

Yang³

et al. 2018

Front. Pharmacol.

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Dihuang powder (DHP) has been used in the traditional Chinese medicine for the treatment of diarrhea in some regions of China. But up to now, the anti-diarrheal activity of DHP haven’t been performed with modern pharmacological technology. This study aims to investigate the quality control, the potential toxicity and anti-diarrheal activity of Dihuang powder in mice. High performance liquid chromatography (HPLC) and thin layer chromatography (TLC) were used to detect five active compounds in DHP for quality control, and the acute toxicity and sub-acute toxicity for 28-day oral administration of DHP were then evaluated. The anti-diarrheal activity was investigated using mouse model. Results showed that the levels of quercetin and berberine in DHP were 0.054 and 0.632 mg/g, respectively, and atractylodin, matrine, and patehouli aleohal were also detected in DHP. At the given doses, DHP was safe in terms of acute and sub-acute toxicity. Meanwhile, DHP exhibited strong anti-diarrheal effects as well as decreased gastrointestinal motility and the secretions induced by Sennae and castor oil in a dose-dependent manner. It could decrease the content of IL-1β, IL-6, and TNF-α in the small intestine, and improve the histopathological changes of small intestine and large intestine induced by Sennae. The antinociceptive and anti-inflammatory activities in vivo also were presented. Based on all of the results, we thought that DHP has anti-diarrheal activity, and could be used to treat diarrhea as well as alleviate the pain and inflammation induced by diarrhea. This study provides a theoretical basis for the clinical use of DHP and may assist in the development of new drugs for the treatment of diarrhea. The mechanism of the anti-diarrheal activity should be investigated in the future.

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Section: Discussionmentioning

confidence: 99%

The Anti-diarrheal Activity of the Non-toxic Dihuang Powder in Mice

Shang¹,

Miao²,

Yang³

et al. 2018

Front. Pharmacol.

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“…Euphorbia humifusa Willd is one of the elements of JTD thought to reduce FG and enhance insulin sensitivity in KK-Ay mice [24]. Additionally, it can inhibit NF-κB activity and down-regulate the production of inflammatory mediators, such as nitric oxide (NO) and TNF-α [25, 26]. Salvia miltiorrhiza Bunge has been reported to improve the quantity and amount of pancreatic islet cells, repair their structure and function, increase glucose transporter 4 (GLUT4) and glycogen synthase protein expression in the skeletal muscle, as well as suppress the secretion of NO, TNF-α, and IL-6 in RAW264.7 macrophages stimulated with LPS [12].…”

Section: Discussionmentioning

confidence: 99%

Jiangtang decoction ameliorate diabetic nephropathy through the regulation of PI3K/Akt-mediated NF-κB pathways in KK-Ay mice

Wang

et al. 2017

Chin Med

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BackgroundJiangtang decoction (JTD) is a China patented drug which contains Euphorbia humifusa Willd, Salvia miltiorrhiza Bunge, Astragalus mongholicus Bunge, Anemarrhena asphodeloides Bunge, and Coptis chinensis Franch. For decades, it has also been used clinically to treat diabetic nephropathy (DN) effectively; however, the associated mechanisms remain unknown. Thus, the present study aimed to examine the protective efficacy of JTD in DN and elucidate the underlying molecular mechanisms.MethodsA diabetic model using KK-Ay mice received a daily administration of JTD for 12 weeks. Body weight, blood glucose, triglycerides (TGs), total cholesterol (TC), urea nitrogen (UN), creatinine (Cr), and microalbumin/urine creatinine (MA/UCREA) was measured every 4 weeks. Furthermore, on the day of the sacrifice, blood, urine, and kidneys were collected to assess renal function according to general parameters. Pathological staining was performed to evaluate the protective renal effect of JTD. In addition, the levels of inflammatory cytokines (tumor necrosis factor-α [TNF-α], interleukin [IL]-6 and intercellular adhesion molecule [ICAM]-1), insulin receptor substrate [IRS]-1, advanced glycation end products [AGEs], and receptor of glycation end products [RAGE] were assessed. Finally, the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway and involvement of nuclear factor-κB (NF-κB) was further analyzed.ResultsAfter 12 weeks of metformin and JTD administration, the mice exhibited a significant amelioration in glucose and lipid metabolism dysfunction, reduced morphological changes in the renal tissue, decreased urinary albumin excretion, and normalized creatinine clearance. JTD treatment also reduced the accumulation of AGEs and RAGE, up-regulated IRS-1, and increased the phosphorylation of both PI3K (p85) and Akt, indicating that the activation of the PI3K/Akt signaling pathway was involved. Additionally, JTD administration reduced the elevated levels of renal inflammatory mediators and decreased the phosphorylation of NF-κB p65.ConclusionsThese results demonstrate that JTD might reduce inflammation in DN through the PI3K/Akt and NF-κB signaling pathways.Electronic supplementary materialThe online version of this article (doi:10.1186/s13020-017-0134-0) contains supplementary material, which is available to authorized users.

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“…In Korea, EuH has been used as a traditional medicinal plant for the treatment of diarrhoea, jaundice, dysentery, enteritis, diabetes, and asthma [12, 13]. Previous phytochemical studies have isolated multiple bioactive compounds from the EuH, including euphorbinoside dehydropicrorhiza acid methyl diester, flavone glucosides, apigenin glycosides, tannins, α-pyrrolidinonoids, lanostane triterpenoids [14–16], exhibiting various pharmacological actions, including antifungal, anti-inflammatory, vasorelaxant, and anti-viral properties [12, 16–18]. …”

Section: Introductionmentioning

confidence: 99%

Euphorbia humifusa Willd exerts inhibition of breast cancer cell invasion and metastasis through inhibition of TNFα-induced MMP-9 expression

Shin

Kim

Jung

et al. 2016

BMC Complement Altern Med

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BackgroundBreast cancer is the most common type of malignancy in women worldwide. Euphorbia humifusa Willd (EuH) is a plant that is widely used as a traditional medicine. However, no systemic studies on the anti-cancer effects of EuH have been reported. The aim of this study is to evaluate the anti-metastatic effect of the EuH.MethodsEthyl acetate fraction was prepared from EuH methanol extracts (EA/EuH). Inhibitory effect of EA/EuH on cell migration was determined using an in vitro scratch-wound healing assay. The anti-invasive activity was determined by in vitro three-dimensional spheroid culture system and in vivo syngenic experimental lung metastasis experiment. Gene expression profiles were analyzed by using RT-PCR, real-time PCR, and luciferase reporter assay systems.ResultsEthyl acetate fraction from the EuH extract (EA/EuH) inhibited the migration and invasive capabilities of highly metastatic MDA-MB-231 breast cancer cells and attenuated syngeneic lung metastasis of mouse 4 T1 breast cancer cells in vivo. Mechanistically, EA/EuH decreased tumor necrosis factor alpha (TNFα)-induced matrix metalloproteinase (MMP)-9 mRNA expression through the inhibition of NF-κB activity in MDA-MB-231 cells.ConclusionEuH may be beneficial in the prevention of invasion and metastasis of early stage breast cancer and can be served as an anti-metastatic agent or adjuvant therapy against metastatic breast cancer.

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Anti-inflammatory components of Euphorbia humifusa Willd.

Cited by 51 publications

References 37 publications

The Anti-diarrheal Activity of the Non-toxic Dihuang Powder in Mice

The Anti-diarrheal Activity of the Non-toxic Dihuang Powder in Mice

Jiangtang decoction ameliorate diabetic nephropathy through the regulation of PI3K/Akt-mediated NF-κB pathways in KK-Ay mice

Euphorbia humifusa Willd exerts inhibition of breast cancer cell invasion and metastasis through inhibition of TNFα-induced MMP-9 expression

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